Effects of flunixin and florfenicol combined with vitamins E and/or C on selected immune mechanisms in cattle under conditions of adaptive stress

• Autorzy: Urban-Chmiel R. , Stachura R. , Hola P. , Puchalski A. , Dec M. , Wernicki A.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to evaluate the effect of flunixin and florfenicol administered in combination with vitamin E or C on selected leukocyte immune mechanisms and on the inflammatory process during the first few weeks in the feedlot. Fifty calves divided into 5 groups (n = 10) received florfenicol and flunixin with vitamin E or C. Blood was collected on the 1st, 3rd , 7th , 14th , 21st , and 28th d of the experiment. Intracellular metabolism (NBT), apoptosis, Chemotaxis, susceptibility to M. haemolytica leukotoxin, and expression of ß₂-integrins were determined in leukocytes. The symptoms of respiratory tract infection were observed in 40% of calves in control group, while in the other groups the morbidity rate ranged from 10% to 20%. Leukocytes showed decreased NBT, and the mean values for apoptosis ranged from 14% to 24%. The lowest percentage of apoptotic cells was observed in the calves that received florfenicol with flunixin and vitamins E and C. The chemotactic activity confirmed the significant inhibitory effect of the preparations on migration of the cells. A significant decrease (P ≤ 0.05) in the susceptibility of leukocy tes to leukotoxin was noted in the group that received florfenicol and flunixin with vitamin E. Expression of ß₂-integrin receptors was the lowest in calves receiving florfenicol with flunixin and vitamin E or C. The application of an antibiotic and a non-steroidal anti-inflammatory drug with antioxidants protected the leukocytes involved in defence against M. haemolytica virulence factors and effectively limited oxidative stress in the calves.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2015
• Tom: 59
• Numer: 2
• Opis fizyczny: p.295-301,fig.,ref.

Twórcy

autor

Urban-Chmiel R.

• Sub-department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Poland

autor

Stachura R.

• Sub-department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Poland
• Agromarina Farm, Kulczyn-Kolonia 48, 22-235 Hansk, Poland

autor

Hola P.

• Agromarina Farm, Kulczyn-Kolonia 48, 22-235 Hansk, Poland

autor

Puchalski A.

• Sub-department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Poland

autor

Dec M.

• Sub-department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Poland

autor

Wernicki A.

• Sub-department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Poland

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Identyfikatory

DOI

10.1515/bvip-2015-0043