Influence of proteins on model lipid membrane studied with application of the BLM-Black Lipid Membrane method

• Autorzy: Reszczynska E. , Trebacz K. , Gruszecki W. L.
• Języki publikacji: EN

Abstrakty

EN

Black Lipid Membrane (BLM) technique was used to examine effects of BSA (Bovine serum albumin) on properties on the model membrane. BLMs were formed with EYPC (L-αPhospatydylocholine from eggs yolk) in n-dekane. BLM studied were performed in phosphate buffer (pH 7.4). Various concentrations of BSA (between 0.01 mg/ml and 2.5×10-3 mg/ml) were used in order to optimize experimental conditions. BSA concentrations above 0.01 mg/ml proved too high as they caused collapse of the lipid membrane. Supplementation of the protein resulted in change of resistance of the lipid membrane. The experiment indicates that the protein incorporates into the lipid membrane.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2014
• Tom: 74
• Numer: 3
• Opis fizyczny: pp.339

Twórcy

autor

Reszczynska E.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie- Skłodowska University, Lublin, Poland
• Department of Biophysics, Institute of Physics, Maria Curie- Skłodowska University, Lublin, Poland

autor

Trebacz K.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie- Skłodowska University, Lublin, Poland

autor

Gruszecki W. L.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie- Skłodowska University, Lublin, Poland
• Department of Biophysics, Institute of Physics, Maria Curie- Skłodowska University, Lublin, Poland