In situ impact of the antagonistic fungal strain, Trichoderma gamsii T30 on the plant pathogenic fungus, Rhizoctonia solani in soil

• Autorzy: Anees M. , Abid M. , Chohan S. , Jamil M. , Ahmed N. , Zhang L. , Rha E.S.
• Języki publikacji: EN

Abstrakty

EN

Rhizoctonia solani is a soil-borne fungus causing a wide range of plants diseases. Trichoderma gamsii strain T30 has previously been reported as antagonistic against R. solani. Although there are a few studies about the influence of Trichoderma strains on the R. solani densityin a pathosystem in the presence of plant hosts, this report for the first time comprehensively describes in situ effects of a T. gamsii strain on the population density of R. solani in the soil microcosmic conditions. The population dynamics of R. solani were followed in the auto-claved and non-autoclaved soils in artificially prepared microcosms up to day 25 after co-inoculation with T. gamsii in the variable ratios (R1/T1; R1/T0.1; R1/T0.01 of R. solani/T. gamsii). The population density of R. solani was evaluated by qPCR. In the autoclaved soil, target DNA copies of R. solani increased in the control samples from 1 × 10⁵ to 6.5 × 10⁶. At R1/T0.01, the number of target DNA copies were not significantly changed until day 11; however, it decreased by around five times at day 25. At R1/T0.1 and R1/T1, the number of DNA copies was reduced to 2.1 × 10⁶ and 7.6 × 10⁵ at day 11, respectively and the reduction was as much as 17 times at day 25. In the non-autoclaved soil, the number of the fungal cells decreased at day 25 whether inoculated or not with Trichoderma indicating a general suppression by the soil microbiome. In brief, T. gamsii significantly inhibited the growth of R. solani in the soil in situ and there was a general suppressive effect of the natural microbiome.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2019
• Tom: 68
• Numer: 2
• Opis fizyczny: p.211-216,fig.,ref.

Twórcy

autor

Anees M.

• Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan

autor

Abid M.

• Department of Plant Pathology, Baha ud Din Zakaria University, Multan, Pakistan

autor

Chohan S.

• Department of Plant Pathology, Baha ud Din Zakaria University, Multan, Pakistan

autor

Jamil M.

• Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

autor

Ahmed N.

• Department of Botany, Mohi ud Din Islamic University AJ and K, Pakistan

autor

Zhang L.

• Northwest A and F University, Yangling, China

autor

Rha E.S.

• Department of Well-being Resources, Sunchon National University, South Korea

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Identyfikatory

DOI

10.33073/pjm-2019-021