Immunohistochemical and histopathological evaluation of malignant pheochromocytoma: a case study

• Autorzy: Mikiewicz M. , Otrocka-Domagala I. , Gesek M. , Pazdzior-Czapula K. , Rotkiewicz T.
• Języki publikacji: EN

Abstrakty

EN

Malignant pheochromocytoma is an uncommon neoplasm in dogs. This study evaluated the histopathological and immunohistochemical profile of malignant pheochromocytoma. The report presents a case of bilateral adrenal gland tumor in a 6-year-old female golden retriever, diagnosed during necropsy. The necropsy also showed changes in other organs, i.e. heart (left ventricular hypertrophic cardiomyopathy, right ventricular dilated cardiomyopathy and infarction foci), lungs (multifocal alveolar emphysema and atelectasis). Metastases were not present. A histological examination showed that neoplastic cells were round, oval, polygonal and spindle with a high degree of anisocytosis and anisokaryosis. The nuclei were small, round, oval and some were divided. The nucleoli were faintly visible, and numbered from 2 to 3. The number of mitotic figures per 400 high power fields ranged from 0 to 2. Immunohistochemical staining using an antibody panel revealed that the tumor cells were strongly positive for chromogranin A, S100 protein and neuron specific enolase, but were negative for Ki67. PCNA expression was observed in some neoplastic cells, especially in those located close to the connective tissue stroma. Malignant pheochromocytoma was diagnosed on the basis of a immunohistochemical examination and tumor morphology.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2015
• Tom: 71
• Numer: 07
• Opis fizyczny: p.453-457,fig.,ref.

Twórcy

autor

Mikiewicz M.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland

autor

Otrocka-Domagala I.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland

autor

Gesek M.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland

autor

Pazdzior-Czapula K.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland

autor

Rotkiewicz T.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland

Bibliografia

• 1. Barthez P. Y., Marks S. L., Woo J., Feldman E. C., Matteucci M.: Pheochromocytoma in dogs: 61 cases (1984-1995). J. Vet. Intern. Med. 1997, 11, 272-278.
• 2. Chatzizisis Y. S., Ziakas A., Feloukidis C., Paramythiotis D., Hadjimiltiades S., Iliadis A., Basdanis G., Styliadis I.: Pheochromocytoma crisis presenting with cardiogenic shock. Herz. 2013, DOI 10.1007/s00059-013-3778-2.
• 3. Gilson S. D., Withrow S. J., Wheeler S. L., Twedt D. C.: Pheochromocytoma in 50 dogs. J. Vet. Intern. Med. 1994, 8, 228-232.
• 4. Gostelow R., Bridger N., Syme H. M.: Plasma-free metanephrine and free normetanephrine measurement for the diagnosis of pheochromocytoma in dogs. J. Vet. Intern. Med. 2009, 27, 83-90.
• 5. Kiupel M., Capen C., Miller M., Smedly R.: Tumors of the adrenal medulla, [in]: Schulman F. Y. (ed.): Histological classification of the tumor of the endocrine system of domestic animals. Armed Forces Institute of Pathology, Washington D.C. 2008, 46-47.
• 6. Kook P. H., Boretti F. S., Hersberger M., Glaus T. M., Reusch C. E.: Urinary catecholamine and metanephrine to creation ratios in healthy dogs at home and a hospital environmental and in 2 dogs with pheochromocytoma. J. Vet. Intern. Med. 2007, 21, 388-393.
• 7. Lee J. H., Lee S. S., Lee J. C., Kim M. S., Choi J. H.: Functional mediastinal pheochromocytoma. Korean J. Thorac. Cardiovasc. Surg. 2013, 46, 88-91.
• 8. Loste A., Borobia M., Lacasta D., Carbonell M., Basurco A., Marca M. C.: Adrenal gland tumors. Different clinical presentations in thee dogs: a case report. Veterinarni Medicina. 2013, 58, 377-384.
• 9. McNicol A. M.: Histopathology and immunohistochemistry of adrenal medullary tumors and paragangliomas. Endocr. Pathol. 2006, 17, 329-336.
• 10. Miller A. D., Masek-Hammerman K., Dalecki K., Mansfield K. G., Westmoreland S. V.: Histologic and immunohistochemical characterization of pheochromocytoma in 6 cotton-top tamarins (Saguinus oedipus). Vet. Pathol. 2009, 46, 1221-1229.
• 11. Pace V., Perentes E., Germann P. G.: Pheochromocytomas and ganglioneuromas in aging rats: morphological and immunohistochemical characterization. Toxicol. Pathol. 2002, 30, 492-500.
• 12. Portela-Gomes G. M., Stridsberg M., Grimelius L., Falkmer U. G., Falkmer S.: Expression of chromogranins A, B, and C (secretogranin II) in human adrenal medulla and in benign and malignant phoechromocytomas. An immunohistochemical study with region-specific antibodies. APMIS. 2004, 112, 663-673.
• 13. Sato T., Kitamura N., Kagawa Y., Hirayama K., Morita M., Kurosawa T., Yoshino T., Taniyama H.: Immunohistochemical evaluation of malignant pheochromocytomas in a wolfdog. Vet. Pathol. 2001, 38, 447-450.
• 14. Tischler A. S., Powers J. F., Alroy J.: Animal models of pheochromocytoma. Histol. Histopathol. 2004, 19, 883-895.
• 15. Thompson L. D. R.: Pheochromocytoma of the Adrenal Gland Scaled Score (PASS) to separate Benign from malignant neoplasms. Am. J. Surg. Pathol. 2002, 26, 551-566.
• 16. Yoshimasa T., Nakao K., Ohtsuki H., Li S., Imura H.: Methionine-enkephalin and leucine-enkephalin in human sympathoadrenal system and pheochromocytoma. J. Clin. Invest. 1982, 69, 643-650.