Cassava (Manihot esculenta Crantz) is a tropical and subtropical plant and susceptible to chilling injury. In this research, a C-repeat binding factor (CBF)-like gene (GenBank accession number JQ339740) has been isolated from cassava, and named as MeCBF1. The full-length DNA of MeCBF1 is 1,037 base pair (bp), without intron. The 5' untranslated region is 102 bp, the 3' untranslated region is 239 bp, and the open reading frame is 696 bp encoding 231 amino acids. The deduced amino acid sequence of MeCBF1 contains two CBF conserved motifs of PKK(P/R)AGRxKFxETRHP and DSxWR. The MeCBF1 shows 83 % homology to the CRT/DRE binding factor 1 from Hevea brasiliensis (Accession no. AAY43213.1). However, in cassava, the MeCBF1 target genes showed low similarity to the CBF/DREB regulated genes in Arabidopsis thaliana. Quantitative real-time PCR showed that the MeCBF1 was highly expressed in stems and leaves, and lowly expressed in roots. In addition, the expression of the MeCBF1 quickly responded to low temperature stress (4°C). These results suggest that, the MeCBF1 is functional in cassava. Further studies on the MeCBF1 might be helpful to reveal molecular mechanism of cassava’s high sensitivity to low temperature.