A comparative study of effects of increasing concentrations of phosphate and phosphite on rice seedlings

• Autorzy: Manna M. , Islam T. , Kaul T. , Reddy C.S. , Fartyal D. , James D. , Reddy M.K.
• Języki publikacji: EN

Abstrakty

EN

While phosphate (Pi) serves as an essential and indispensible plant nutrient, phosphite (Phi) acts as a potent herbicide. Despite their differential influence on plants, both the ions can attenuate phosphate starvation responses (PSRs). We analyzed and compared Pi and Phi uptake and accumulation, attenuation of PSRs and the morphological and physiological responses of the rice seedlings in response to the increasing concentrations of Pi and Phi. Our study revealed that increasing levels of Phi led to pronounced reduction in shoot and root mass in rice seedlings in comparison to similar Pi treatments. Phi inhibited root hair and root formation at 5 and 30 mM Phi concentrations, respectively. Whereas, higher Pi concentrations (40 and 50 mM) affected only root hair elongation. Increasing Phi dose led to drastic reduction in chlorophyll content which was not so in case of Pi. There was inverse relationship between external Pi/Phi level and anthocyanin content of the leaves. In comparison to 20 mM Pi treatment, similar dose of Phi led to significant downregulation of Pi transportersin both leaves and roots. Rice seedlings were found to accumulate mmol and lmol levels of Pi and Phi, respectively. Comparison of various PSR parameters revealed that in comparison to Pi, Phi exhibited greater degree of attenuation of PSRs. Lesser Phi accumulation and greater attenuation of PSRs by Phi indicate plant’s adaption to restrict entry of this toxic ion inside cells.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 12
• Opis fizyczny: Article: 258 [10 p.], fig.,ref.

Twórcy

autor

Manna M.

• Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

autor

Islam T.

• Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

autor

Kaul T.

• Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

autor

Reddy C.S.

• Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

autor

Fartyal D.

• Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

autor

James D.

• Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

autor

Reddy M.K.

• Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

Bibliografia

• Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15
• Berkowitz O, Jost R, Pearse SJ, Lambers H, Finnegan PM, Hardy GE, O’Brien PA (2011) An enzymatic fluorescent assay for the quantification of phosphite in a microtiter plate format. Anal Biochem 412:74–78
• Borch K, Bouma TJ, Lynch JP, Brown KM (1999) Ethylene: a regulator of root architectural responses to soil phosphorus availability. Plant, Cell Environ 22:425–431
• Cai H, Xie W, Zhu T, Lian X (2012) Transcriptome response to phosphorus starvation in rice. Acta Physiol Plant 34:327–341
• Chu W, Zhengquan W, Hailong S, Shenglei G (2006) Effects of different concentrations of nitrogen and phosphorus on chlorophyll biosynthesis, chlorophyll a fluorescence, and photosynthesis in Larix olgensis seedlings. Front For China 2:170–175
• Ciereszko I, Zebrowska E, Ruminowicz M (2011) Acid phosphatases and growth of barley (Hordeum vulgare L.) cultivars under diverse phosphorus nutrition. Acta Physiol Plant 33:2355–2368
• Danova-Alt R, Dijkema C, De Waard P, Kock M (2008) Transport and compartmentation of phosphite in higher plant cells—kinetic and P nuclear magnetic resonance studies. Plant, Cell Environ 31:1510–1521
• de Graaf RM, Schwartz AW (2000) Reduction and activation of phosphate on the primitive earth. Orig Life Evol Biosph 30:405–410
• Dolan L (2001) The role of ethylene in root hair growth in Arabidopsis. J Plant Nutr Soil Sci 164:141–145
• Fang Z, Shao C, Meng Y, Wu P, Chen M (2009) Phosphate signalling in Arabidopsis and Oryza sativa. Plant Sci 176:170–180
• Gilbert GA, Knight JD, Vance CP, Allan DL (2000) Proteoid root development of phosphorus deficient lupin is mimicked by auxin and phosphonate. Ann Bot 85:921–928
• Gould KS, Markham KR, Smith RH, Goris JJ (2000) Functional role of anthocyanins in leaves of Quintinia serrata A. Cunn J Expt Bot 51:1107–1115
• Hirosse EH, Creste JE, Custo´dio CC, Machado-Neto NB (2012) In vitro growth of sweet potato fed with potassium phosphite. Acta Sci Agron 34:85–91
• Lanzetta PA, Alvarez LJ, Reinach PS, Candida OA (1979) An improved assay for nanomole amounts of inorganic phosphate. Anal Biochem 100:95–97
• Lee RB, Ratcliffe RG, Southon TE (1990) 31P NMR measurements of the cytoplasmic and vacuolar Pi content of mature maize roots: relationships with phosphorus status and phosphate fluxes. J Exp Bot 41:1063–1078
• Lim JH, Chung IM, Ryu SS, Park MR, Yun SJ (2003) Differential response of rice acid phosphatase activities and isoforms to phosphorus deprivation. J Biochem Mol Biol 36:597–602
• Lopez-Arredondo DL, Herrera-Estrella L (2012) Engineering phosphorus metabolism in plants to produce a dual fertilization and weed control system. Nat Biotechnol 30:889–893
• Lopez-Bucio J, Cruz-Ramirez A, Herrera-Estrella L (2003) The role of nutrient availability in regulating root architecture. Curr Opin Plant Biol 6:280–287
• Ma Z, Baskin TI, Brown KM, Lynch JP (2003) Regulation of root elongation under phosphorus stress involves changes in ethylene responsiveness. Plant Physiol 131:1381–1390
• Miller SS, Liu JQ, Allan DL, Menzhuber CJ, Fedorova M, Vance CP (2001) Molecular control of acid phosphatase secretion into the rhizosphere of proteoid roots from phosphorus-stressed white lupin. Plant Physiol 127:594–606
• Murray JR, Hackett WP (1991) Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L. Plant Physiol 97:343–351
• Nilsson L, Muller R, Nielsen TH (2010) Dissecting the plant transcriptome and the regulatory responses to phosphate deprivation. Physiol Plant 139:129–143
• Peret B, Rybel BD, Casimiro I, Benkova E, Swarup R, Laplaze L, Beeckman T, Bennett MJ (2009) Arabidopsis lateral root development: an emerging story. Trends Plant Sci 14:399–408
• Plaxton WC, Tran HT (2011) Metabolic adaptations of phosphatestarved plants. Plant Physiol 156:1006–1015
• Schachtman DP, Reid RJ, Ayling SM (1998) Phosphorus uptake by plants: from soil to cell. Plant Physiol 116:447–453
• Seo HM, Jung Y, Song S, Kim Y, Kwon T, Kim DH, Jeung SJ, Yi YB, Yi G, Nam MH, Nam J (2008) Increased expression of OsPT1, a high-affinity phosphate transporter, enhances phosphate acquisition in rice. Biotechnol Lett 30:1833–1838
• Syers JK, Johnston AE, Curtin D (2008) Efficiency of soil and fertilizer phosphorus use. Reconciling changing concepts of soil phosphorus behaviour with agronomic information. FAO Fertilizer and Plant Nutrition Bulletin No. 18. FAO, Rome, p 110
• Thao HTB, Yamakawa T, Myint AK, Sarr PS (2008a) Effects of phosphite, a reduced form of phosphate, on the growth and phosphorus nutrition of spinach (Spinacia oleracea L.). Soil Sci Plant Nutr 54:761–768
• Thao HTB, Yamakawa T, Shibata K, Sarr PS, Myint AK (2008b) Growth response of komatsuna (Brassica rapa var. peruviridis)to root and foliar applications of phosphite. Plant Soil 308:1–10
• Ticconi CA, Delatorre CA, Abel S (2001) Attenuation of phosphate starvation responses by phosphite in Arabidopsis. Plant Physiol 127:963–972
• Tran HT, Hurley BA, Plaxton WC (2010) Feeding hungry plants: the role of purple acid phosphatases in phosphate nutrition. Plant Sci 179:14–27
• Veljanovski V, Vanderbeld B, Knowles VL, Snedden WA, Plaxton WC (2006) Biochemical and molecular characterization of AtPAP26, a vacuolar purple acid phosphatase up-regulated in phosphate-deprived Arabidopsis suspension cells and seedlings. Plant Physiol 142:1282–1293
• Williamson LC, Ribrioux SPCP, Fitter AH, Leyser HMO (2001) Phosphate availability regulates root system architecture in Arabidopsis. Plant Physiol 126:875–882
• Wu C, Wang Z, Sun H, Guo S (2006) Effects of different concentrations of nitrogen and phosphorus on chlorophyll biosynthesis, chlorophyll a fluorescence, and photosynthesis in Larix olgensis seedlings. Front For China 1:170–175
• Zebrowska E, Bujnowska E, Ciereszko I (2012) Differential responses of oat cultivars to phosphate deprivation: plant growth and acid phosphatase activities. Acta Physiol Plant 34:1251–1260
• Zhang K, Liu H, Tao P, Chen H (2014) Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves. PLoS ONE 9(5):e98215. Doi:10. 1371/journal.pone.0098215

Identyfikatory

DOI

10.1007/s11738-015-2016-3