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2014 | 54 | 3 |

Tytuł artykułu

Overexpression of cytochrome P450 CYP6B7 mediated pyrethroid resistance in Indian strains of the cotton bollworm, Helicoverpa armigera (Hübner)

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The role of cytochrome P450 monooxygenase in pyrethroid resistance was studied in different strains of the cotton bollworm, Helicoverpa armigera, from India. Filed collected strains of Nagpur and Delhi were compared to the laboratory reared population. The results showed a high resistance to deltamethrin, α-cypermethrin, and β-cyfluthrin. The results also showed that this resistance could be reduced by using piperonyl butoxide (PBO). The Nagpur and Delhi strains were found to have a 2.40 and 1.79 fold higher monooxygenase activity compared to a susceptible strain. A strong, positive correlation between monooxygenase activity and pyrethroid resistance was observed (r = 0.86 - 0.98). The relative expression of the housekeeping gene, EF-1α, and three P450 genes, was studied in the 5th instar larval midgut of the three strains. Out of the three P450 genes examined, expression of CYP6B7 mRNA was not detected in the midgut of the susceptible strain though it was highly expressed in the resistant strains. The midgut of the Nagpur strain had a 2.60 fold overexpression of CYP6B7 mRNA compared to the moderately resistant, Delhi strain. The mRNA of CYP4G8 and CYP6B2 were not overexpressed in either the Nagpur or Delhi strain. The results indicated that the elevated cytochrome P450 monooxygenase activity is associated with pyrethroid resistance in Indian strains of H. armigera, and CYP6B7 could be the P450 form responsible for pyrethroid resistance.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

54

Numer

3

Opis fizyczny

p.287-293,ref.

Twórcy

  • Department of Plant Protection, Faculty of Agriculture, Sohag University, Elkawther City, Sohag, 82749, Egypt
  • Division of Plant Protection, Central Institute for Cotton Research, Nagpur, India
autor
  • Division of Plant Protection, Central Institute for Cotton Research, Nagpur, India
autor
  • Division of Plant Protection, Central Institute for Cotton Research, Nagpur, India
  • Division of Entomology, Indian Agricultural Research Institute, New Delhi, 110012, India

Bibliografia

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Bibliografia

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