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2009 | 31 | 3 |

Tytuł artykułu

Tissue-specific regulation of rice molybdenum cofactor sulfurase gene in response to salt stress and ABA

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Molybdenum-containing aldehyde oxidase is a key enzyme for catalyzing the final step of abscisic acid (ABA) biosynthesis in plants. Sulfuration of the molybdenum cofactor (MoCo) is an essential step for activating aldehyde oxidase. The molybdenum cofactor sulfurase (MCSU) that transfers the sulfur ligand to aldehyde oxidase-bound MoCo is thus considered an important factor in regulating the ABA levels in plant tissues. In this study, we identified the rice MCSU cDNA (OsMCSU), which is the first MCSU gene cloned in monocot species. According to the functional domain analysis of the predicted amino acid sequence, the OsMCSU protein contains a Nifs domain at its N-terminus and a MOSC domain at the C-terminus. Expression of the OsMCSU gene was up-regulated by salt stress in root tissues of rice seedlings, but this effect was not observed in leaf tissues. In roots, regulations of OsMCSU expressions could be mediated by both ABAdependent and ABA-independent signaling pathways under salt stress condition.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

31

Numer

3

Opis fizyczny

p.545-551,fig.,ref.

Twórcy

autor
  • Department of Agronomy, National Taiwan University, No. 1, Sect. 4, Roosevelt Rd, Taipei 106, Taiwan
autor
  • Department of Agronomy, National Taiwan University, No. 1, Sect. 4, Roosevelt Rd, Taipei 106, Taiwan
autor
  • Department of Agronomy, National Taiwan University, No. 1, Sect. 4, Roosevelt Rd, Taipei 106, Taiwan

Bibliografia

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  • Bittner F, Oreb M, Mendel RR (2001) ABA3 is a molybdenum cofactor sulfurase required for activation of aldehyde oxidase and xanthine dehydrogenase in Arabidopsis thaliana. J Biol Chem 276:40381–40384. doi:10.1074/jbc.C100472200
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  • Gonzalez-Guzman M, Apostolova N, Belles JM, Barrero JM, Piqueras P, Ponce MR, Micol JL, Serrano R, Rodriguez PL (2002) The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde. Plant Cell 14:1833–1846. doi:10.1105/tpc.002477
  • Heidenreich T, Wollers S, Mendel RR, Bittner F (2005) Characterization of the NifS-like domain of ABA3 from Arabidopsis thaliana provides insight into the mechanism of molybdenum cofactor sulfuration. J Biol Chem 280:4213–4218. doi:10.1074/jbc.M411195200
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  • Xiong L, Lee H, Ishitani M, Zhu JK (2002) Regulation of osmotic stress-responsive gene expression by the LOS6/ABA1 locus in Arabidopsis. J Biol Chem 277:8588–8596. doi:10.1074/jbc. M109275200
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Bibliografia

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