Molecular diversity of Clostridium botulinum and phenotypically similar strains

• Autorzy: Grenda T. , Kukier E. , Sieradzki Z. , Goldsztejn M. , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

This study was undertaken to examine phenotypic and genetic features of strains preliminary classified as Clostridium botulinum species. The phenotypic characteristics were assessed with different culture media and biochemical tests. The genetic characterization included detection of botulinum toxin genes by PCR and macrorestriction analysis with SmaI, XhoI and SacII by PFGE (Pulsed-field Gel Electrophoresis). Despite similar biochemical properties of all analysed strains, only 47% of them contained genes determining toxicity specific to C. botulinum species. The most valuable differentiation of C. botulinum and C. botulinum-like strains was obtained after SmaI digestion. The highest affinity was observed among C. botulinum type B profiles which was even up to 100%. It was found 100% of affinity between C. botulinum and C. botulinum-like strains, however, the similarity among C. botulinum and C. botulinum-like was generally lower than 80%.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 4
• Opis fizyczny: p.831-840,fig.,ref.

Twórcy

autor

Grenda T.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kukier E.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Sieradzki Z.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Goldsztejn M.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kwiatek K.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

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Identyfikatory

DOI

10.1515/pjvs-2016-0104