Oxidant/antioxidant status assessment of blood in selected equine diseases

• Autorzy: Krumrych W. , Zbylut J. , Golynski M. , Markiewicz H.
• Języki publikacji: EN

Abstrakty

EN

The objective of the study was to assess ratio of oxidation to antioxidation in the blood of horses in selected equine diseases. The study was conducted on 47 Polish half-breed horses (36 mares, six stallions, and five geldings) aged 2 to 16 years. Nineteen mares were diagnosed with endometritis, seven horses with symptoms of colic, and six with upper respiratory tract infection. The remaining clinically healthy horses (n=15) served as control. The following parameters were measured: oxygen metabolism in neutrophils using a chemiluminescence (CL) method, total antioxidant status (TAS) of blood plasma, as well as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase activity in whole blood. The results demonstrate increased CL of neutrophils in mares with endometritis, as well as in horses with symptoms of colic. This finding was associated with decreased values of SOD, GPx, and TAS (chiefly in mares with endometritis), which suggests oxidative stress. These results underscore the importance of a broader use of oxidant/antioxidant status assessment in studies of the pathogenesis and pathomechanisms of equine diseases.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 2
• Opis fizyczny: p.225-230,ref.

Twórcy

autor

Krumrych W.

• Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, 85-090 Bydgoszcz, Poland

autor

Zbylut J.

• Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, 85-090 Bydgoszcz, Poland

autor

Golynski M.

• Department of Internal Diseases of Farm Animals and Horses, Department and Clinic of Animal Internal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 20-950 Lublin, Poland

autor

Markiewicz H.

• Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, 85-090 Bydgoszcz, Poland

Bibliografia

• 1. Asbury A.C., Hansen P.J.: Effects of susceptibility of mares to endometritis and stage of cycle on phagocytic activity of uterine-derived neutrophils. J Reprod Fert 1987, 35, 311-316.
• 2. Bartosz G.: Druga twarz tlenu. Wolne rodniki w przyrodzie. Edited by PWN, Warszawa, 2004, pp. 99-239.
• 3. Benbarek H., Deby-Dupont G., Deby C., Serteyn D.: 20. Direct stimulation of the oxidative activity of isolated equine neutrophils by TNF-alpha and IL-lbeta. Vet Immunol Immunopathol 2008, 121, 101-106.
• 4. Deaton C.M., Marlin D.J., Deaton L., Smith N.C., Harris P.A., Schroter R.C., Kelly F.J.: Comparison of the antioxidant status in tracheal and bronchoalveolar epithelial lining fluids in recurrent airway obstruction. Equine Vet J 2006, 38, 417-422.
• 5. Delguste C., De Moffarts B., Kirschvink N., Art T., Pincemail J., Defraigne J.O., Amory H., Lekeux P.: Change in blood antioxidant status of horses moved from a stable following diagnosis of equine motor neuron disease. Can Vet J 2007, 48. 1165-1167.
• 6. Dimock A.N., Siciliano P.D., McIlwraith C.W.: Evidence supporting an increased presence of reactive oxygen species in the diseased equine joint. Equine Vet J 2000, 32, 439-443.
• 7. Evans P., Halliwell B.: Micronutrients: oxidant/ antioxidant status. Br J Nutr 2001, 85, 67-74.
• 8. Grulke S., Benbarek H., Caudron I., Deby-Dupont G., Mathy-Hartert M., Farnir F., Deby C., Lamy M., Serteyn D.: Plasma myeloperoxidase level and polymorphonuclear leukocyte activation in horses suffering from large intestinal obstruction requiring surgery: preliminary results. Can J Vet Res 1999, 63, 142-147.
• 9. Hanafi E.M., Ahmed W.M., Sherein I., Moez A.E., Khadrawy H.H.E., Hameed A.E.: Effect of clinical endometritis on ovarian activity and oxidative stress status in Egyptian Buffalo-Cows. American-Eurasian J Agrie Environ Sci 2008, 4, 530-536.
• 10. Kimura S., Yonemura T., Kaya H.: Increased oxidative product formation by blood polymorphonuclear leukocytes in human periodontal disease. J Periodont Res 1993, 28, 197-203.
• 11. Kirschvink N., Smith N., Fiévez L., Bougnet V., Art T., Degand G., Marlin D., Roberts C., Genicot B., Lindsey P., Lekeux P.: Effect of chronic airway inflammation and exercise on pulmonary and systemic antioxidant status of healthy and heaves-affected horses. Equine Vet J 2002, 34, 563-571.
• 12. Kirschvink N., De Moffarts B., Lekeux P.: The oxidant/antioxidant equilibrium in horses. Vet J 2008, 177, 178-191.
• 13. Kooreman K., Babbs C., Fessler J.: Effect of ischemia and reperfusion on oxidative processes in the large colon and jejunum of horses. Am J Vet Res 1998, 59, 340-346.
• 14. Krumrych W.: Wskaźniki laboratoryjne krwi koni - wartości referencyjne i interpretacja. National Veterinary Research Institute, Pulawy, Poland 2003, pp. 12-14.
• 15. Krumrych W., Wiśniewski E.: The influence of selected stimulators on chemiluminescence of peripheral blood neutrophils in horses. Med Weter 2006, 62, 204-206.
• 16. Krumrych W., Danek J.: Chemiluminescence of peripheral blood neutrophils in mares with endometritis. Bull Vet Inst Pulawy 2012, 56, 51-56.
• 17. Lojek A., Ciz M., Marnila P., Duskova M., Lilius E.M.: Measurement of whole blood phagocyte chemiluminescence in the Wistar rat. J Biolum Chemilum 1997, 12, 225-231.
• 18. Magnusson U., Holst II.: Assaying granulocyte phagocytosis by chemiluminescence: Effect of storage time and temperature of blood samples. J Vet Med B 1998, 45, 217-222.
• 19. Mills P.C., Higgins A.J.: Oxidant injury, nitric oxide and pulmonary vascular function: implications for the exercising horse. Vet J 1997, 153, 125-148.
• 20. Mohamed H.E.: Antioxidant status and the degree of oxidative stress in dromedary (Camelus dromedaries) with or without endometritis. Vet Res 2008, 2, 1-2.
• 21. Neville R.F., Hollands T., Collins S.N., Keyte F.V.: Evaluation of urinary TBARS in normal and chronic laminitic ponies. Equine Vet J 2004, 36, 292-294.
• 22. Noschka E., Werre S.R., Crisman M.V., Thatcher C.D., Milne G.L., Dahlgren L.A.: Implications of urine F2- isoprostane metabolite concentration in horses with colic and its potential use as a predictor for surgical intervention. Equine Vet J 2011, 43, 34-41.
• 23. Papp Z., Smits J.E.G.: Validation and novel applications of the whole-blood chemiluminescence assay of innate immune function in wild vertebrates and domestic chickens. J Wildlife Dis 2007, 43, 623-634.
• 24. Peterhans E.: Oxidants and antioxidants in viral diseases - metabolic regulation and autotoxicity. In: Natural Antioxidants in Human Health and Disease, edited by B. Frei, Academic Press, San Diego, 1994, pp. 489-514.
• 25. Safronova V.G., Matveeva N.K., Maltseva V.N., Kasabulatov N.M., Avkhacheva N.V., Van'ko L.V., Sukhikh G.T.: Regulation of reactive oxygen species production by peripheral blood neutrophils from women with postpartum endometritis. Bull Exp Biol Med 2005, 140, 205-208.
• 26. Splettstoesser W.D., Schuff-Werner P.: Oxidative stress in phagocytes - "The enemy within". Microsc Res Tech 2002, 57, 441-455.
• 27. Triggiani M., De Marino V., Sofia M., Faraone S., Ambrosio G., Carratu L., Marone G.: Characterization of platelet-activating factor acetylhydrolase in human bronchoalveolar lavage. Am J Respir Crit Care Med 1997, 156, 94-100.
• 28. Weiss D.J., Evanson O.A.: Evaluation of lipopolysaccharide-induced activation of equine neutrophils. Am J Vet Res 2002, 63, 811-815.
• 29. Weiss D.J., Evanson O.A.: Evaluation of activated neutrophils in the blood of horses with colic. Am J Vet Res 2003, 64, 1364-1368.
• 30. Yaralioglu-Gurgoze S., Cetin H., Cen O., Yilmaz S., Atli M.O.: The changes of malondialdehyde levels and glutathione peroxidase activity in purebred Arabian mares with endometritis. Vet J 2005, 170, 135-137.
• 31. Yu B.P.: Cellular defenses against damage from reactive oxygen species. Physiol Rev 1994, 74, 139-162.