Detection and molecular characterization of the Iris severe mosaic virus-Ir isolate from Iran

• Autorzy: Nateqi M. , Habibi M.K. , Dizadji A. , Parizad S.
• Języki publikacji: EN

Abstrakty

EN

Iris belongs to the Iridaceae family. It is one of the most important pharmaceutical and ornamental plants in the world. To assess the potyvirus incidence in natural resources of iris plants in Iran, Antigen Coated-Plate ELISA (ACP-ELISA) was performed on 490 symptomatic rhizomatous iris leaf samples, which detected the potyvirus in 36.7% of the samples. Genomic 3’ end of one mechanically non-transmitted potyvirus isolate, comprising a 3’ untranslated region (390 bp) and C-terminus of the coat protein (CP) gene (459 bp), was amplified by reverse transcription polymerase chain reaction (RT-PCR), which was ligated into pTG19-T vector. The nucleotide sequence of amplicons was compared with related sequences, using Blastn software available at NCBI GenBank, and showed the highest similarity with Iris severe mosaic virus (ISMV) isolates. The nucleotide and deduced amino acid sequence of the CP C-terminus region was more than 83% identical with other ISMV isolates, therefore this isolate was designated as ISMV-Ir. This new ISMV isolate is closely related to the Chinese ISMV-PHz in phylogenetic analysis, based on the partial nucleotide and deduced amino acid sequence of the CP region. This is the first report of ISMV occurrence on Iris spp. in Iran.

Słowa kluczowe

EN

detection; molecular characteristics; iris severe mosaic virus; isolate; phylogenesis; iris; Iridaceae; ornamental plant; Iran

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2015
• Tom: 55
• Numer: 3
• Opis fizyczny: p.235-240,fig.,ref.

Twórcy

autor

Nateqi M.

• Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

autor

Habibi M.K.

• Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

autor

Dizadji A.

• Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

autor

Parizad S.

• Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

Bibliografia

• Adams M.J., Antoniw J.F., Fauquet C.M. 2005. Molecular criteria for genus and species discrimination within the family Potyviridae. Archives of Virology 150 (3): 459–479.
• Alper M., Salomon R., Loebenstein G. 1984. Gel electrophoresis of virus-associated polypeptides for detecting viruses in bulbous irises. Phytopathology 74 (8): 960–962.
• Asjes C.J. 1979. Viruses and virus diseases in Dutch bulbous irises (Iris hollandica) in the Netherlands. Netherlands Journal of Plant Pathology 85 (6): 269–279.
• Atreya P.L., Atreya C.D., Pirone T.P. 1991. Amino acid substitutions in the coat protein result in loss of insect transmissibility of a plant virus. Proceedings of the National Academy of Sciences 88 (17): 7887–7891.
• Barnett O.W., de Zoeten G.A., Gaard G. 1971. Bearded iris mosaic virus: transmission, purification, inclusions, and its differentiation from bulbous iris mosaic. Phytopathology 61 (8): 926–932.
• Brierley P., McWhorter F.P. 1936. A mosaic disease of iris. Journal of Agricultural Research 53 (8): 621–635.
• Brunt A.A., Derks A.F.L.M., Barnett O.W. 1988. Iris severe mosaic virus. Commonwealth Mycological Institute. Association of Applied Biologists. Descriptions of Plant Viruses No. 338. Available on: http://www.dpvweb.net/dpv/showdpv. php?dpvno=338 [Accessed: November 15, 2014]
• Brunt A.A., Phillips S. 1980. The detection, separation from naturally occurring complexes, and partial characterisation of four aphid-borne viruses infecting bulbous iris. ISHS Acta Horticulturae 109: 503–508.
• Caiola M.G., Canini A. 2010. Looking for saffron (Crocus sativus L.) parents. In: “Saffron” (A.M. Husaini, ed.). Functional Plant Science and Biotechnology 4 (Special Issue 2): 1–14.
• Caiola M.G., Faoro F. 2011. Latent virus infections in Crocus sativus and Crocus cartwrightianus. Phytopathologia Mediterranea 50 (2): 175–182.
• D’Agostino N., Pizzichinin D., Chiousano M.L., Giuliano G. 2007. An EST database from saffron stigmas. BMC Plant Biology 7: 53–60.
• De Munk W.J., Schipper J. 1993. Iris, bulbous and rhizomatous. p. 349–379. In: The Physiology of Flower Bulbs (A. De Hertogh, M. Le Nard, eds.), Elsevier Science, Amsterdam, The Netherlands, 812 pp.
• Derks A.F.L.M. 1985. Recent advances in bulb virus research. Acta Horticulturae 164: 281–289.
• Derks A.F.L.M, Hollinger T.C. 1986. Similarities of and differences between potyviruses from bulbous and rhizomatous irises. ISHS Acta Horticulturae 177: 555–561.
• Derks A.F.L.M, Vink-van den Abeele J.L., Muller P.J. 1980. Bean yellow mosaic virus in some iridaceous plants. ISHS Acta Horticulturae 110: 31–37.
• Edwardson J.R., Chrisitie R.G., Ko N.J. 1984. Potyvirus cylindrical inclusions–subdivision-IV. Phytopathology 74 (9): 1111–1114.
• Feinberg A.P., Vogelstein B. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132 (1): 6–13.
• Hammond J., Derks A.F.L.M., Barnett O.W., Lawson R.H., Brunt A.A., Inouye N., Allen T.C. 1985. Viruses infecting bulbous iris: a clarification of nomenclature. ISHS Acta Horticulturae 164: 395–397.
• Harrison B.D., Robinson D.J., Tyrrell D.A.J. 1988. Molecular variation in vector-borne plant viruses: epidemiological significance. Philosophical Transactions of the Royal Society of London, Series B, Biological Sciences 321 (1207): 447–462.
• Hasiów-Jaroszewska B., Rymelska N., Borodynko N., Pospieszny H. 2013. Biological and molecular characterization of the polish Zucchini yellow mosaic virus isolates. Acta Scientiarum Polonorum, Hortorum Cultus 12 (2): 75–85.
• Inouye N., Mitsuhata K. 1978. Turnip mosaic virus isolated from Iris. Nogaku Kenkyu 57 (1): 1–16. (in Japanese)
• Joisson C., Dubs M.C., Briand J.P., Van Regenmortel M.H. 1992. Detection of potyviruses with antisera to synthetic peptides. Research in Virology 143 (3): 167–178.
• Kohlein F. 1987. Iris. Timber Press, Portland, Oregon, 370 pp.
• Langeveld S.A., Dore J.M., Memelink J., Derks A.F.L.M., van der Vlugt C.I.M., Asjes C.J., Bol J.F. 1991. Identification of potyviruses using the polymerase chain reaction with degenerate primers. Journal of General Virology 72 (7): 1531–1541.
• Miglino R., Jodlowska A., van Schadewijk A.R. 2005. First report of Narcissus mosaic virus infecting Crocus spp. cultivars in the Netherlands. Plant Disease 89 (3): 342.
• Russo M., Martelli G.P., Cresti M., Ciampolini F. 1979. Bean yellow mosaic virus in saffron. Phytopathologia Mediterranea 18: 189–191.
• Sambrook J., Fritsch E.F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual (2nd ed.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1626 pp.
• Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30 (12): 2725– 2729.
• Van der Vlugt C.I.M. 1994. Distribution and multiplication of Iris severe mosaic potyvirus in bulbous Iris in relation to metabolic activity: Implications for ISMV detection. Ph.D. thesis, Bulb Research Centre Lisse, Dutch Flower bulb Industry, Netherland, 130 pp.
• Van der Vlugt C.I.M., Langeveld S.A., Goldbach R.W. 1994. Molecular cloning and sequence analysis of 3’-terminal region of Iris severe mosaic virus RNA. Archives of Virology 136: 397–406.
• Yan S., Qin Z., Jin L., Chen J. 2010. A new isolate of Iris severe mosaic virus causing yellow mosaic in Iris ensata Thunb. Journal of Nanoscience and Nanotechnology 10 (2): 726–730.

Identyfikatory

DOI

10.1515/jppr-2015-0032