Identification of non-pathogenic fungi of rice and the evaluation of their effect on biological control of Pyricularia grisea, the causal agent of rice blast disease in vitro

• Autorzy: Safari Motlagh M.R. , Usefipoor P.

Warianty tytułu

PL

Identyfikacja niepatogenicznych grzybów ryżu i ocena ich wpływu na zwalczanie biologiczne Pyricularia grisea powodujacego zarazę ryżu in vitro

• Języki publikacji: EN

Abstrakty

EN

Rice blast disease, caused by Pyricularia grisea, is an important rice disease in Iran and throughout the world. Out of 150 infected samples collected from paddy fields of Guilan province, 57 fungal isolates were isolated for which PDA and W.A media were used. Morphological characteristics were used for identification of these fungi. It was found out that the isolated fungi were belonged to Pyricularia grisea, Ulocladium alternariae, Ulocladium cf. alternariae, Ulocladium cf. consortiale, Ulocladium sp., Curvularia pallescens, Preussia sp., Epicoccum sp. and Trichoderma harzianum. According to pathogenicity tests, all isolates of P. grisea were proved to be pathogenic on rice and fungal isolates belonging to other fungal genera which did not cause disease on rice were selected for biological control studies and to do so, were used different methods. Results showed that eight isolates of T. harzianum, U. cf. consortiale, Ulocladium sp., Epicoccum sp., Preussia sp., C. pallescens, U. alternariae and U. cf. alternariae, had the highest inhibitory effect in mycelial growth of P. grisea, respectively. In hyperparasitism test, hyphae of T. harzianum did not coil around the mycelium of P. grisea. In these experiments, T. harzianum and U. cf. consortiale isolates had the highest inhibitory effect on growth rate of P. grisea and was known as the most effective isolates. Based on results, volatile metabolites method was best method in control of P. grisea in vitro.

PL

Zaraza ryżu powodowana przez Pyricularia grisea jest ważną chorobą ryżu w Iranie i na całym świecie. Ze 150 zakażonych próbek zebranych z pól ryżowych w prowincji Guilan, wyizolowano 57 izolatów grzybów, dla których zastosowano pożywki PDA i W.A. Do identyfikacji tych grzybów użyto cech morfologicznych. Stwierdzono, że wyizolowane izolaty grzybów należą do Pyricularia grisea, Ulocladium alternariae, Ulocladium cf. alternariae, Ulocladium cf. consortiale, Ulocladium sp., Curvularia pallescens, Preussia sp., Epicoccum sp. oraz Trichoderma harzianum. Na podstawie testów patogeniczności udowodniono, że wszystkie izolaty P. grisea są chorobotwórcze wobec ryżu, a izolaty grzybów należące do innych rodzajów, które nie powodowały choroby ryżu, wyselekcjonowano do biologicznych badań kontrolnych, stosując w tym celu różne metody. Wywnioskowano, że osiem izolatów: T. harzianum, U. cf. consortiale, Ulocladium sp., Epicoccum sp., Preussia sp., C. pallescens, U. alternariae i U. cf. alternariae miały najbardziej hamujący wpływ na wzrost grzybni P. grisea. Izolaty T. harzianum i U. cf. consortiale miały najsilniejsze działanie inhibicyjne wobec wskaźnika wzrostu P. grisea i uznano je za najbardziej skuteczne izolaty. Na podstawie wyników stwierdzono, że metoda badania frakcji lotnej metabolitów jest najlepszą metodą zwalczania P. grisea in vitro.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2016
• Tom: 15
• Numer: 6
• Opis fizyczny: p.69-86,fig.,ref.

Twórcy

autor

Safari Motlagh M.R.

• Department of Plant Pathology, Faculty of Agriculture, Rasht Branch, Islamic Azad University, Rasht, Guilan Province PO box 41335- -3516, Iran

autor

Usefipoor P.

• Damghan Branch, Islamic Azad University, Damghan, Semnan province, Iran

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