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2012 | 34 | 5 |
Tytuł artykułu

Abscisic acid induces heat tolerance in chickpea (Cicer arietinum L.) seedlings by facilitated accumulation of osmoprotectants

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The gradual rise of global temperature is of major concern for growth and development of crops. Chickpea (Cicer arietinum L.) is a heat-sensitive crop and hence experiences damage at its vegetative and reproductive stages. Abscisic acid (ABA), a stress-related hormone, is reported to confer heat tolerance, but its mechanism is not fully known, especially whether it involves osmolytes (such as proline, glycine betaine and trehalose) in its action or not. Osmolytes too have a vital role in saving the plants from injurious effects of heat stress by multiple mechanisms. In the present study, we examined the interactive effects of ABA and osmolytes in chickpea plants grown hydroponically at varying temperatures of 30/25°C (control), 35/30, 40/35 and 45/40°C (as day/night (12 h/12 h)): (a) in the absence of ABA; (b) with ABA; and (c) in the presence of its biosynthetic inhibitor fluridone (FLU). The findings indicated severe growth inhibition at 45/40°C that was associated with drastic reduction in endogenous ABA and osmolytes compared to the unstressed plants suggesting a possible relationship between them. Exogenous application of ABA (2.5 µM) significantly mitigated the seedling growth at 40/35 and 45/40°C, while FLU application intensified the inhibition. The increase in growth by ABA at stressful temperature was associated with enhancement of endogenous levels of ABA and osmolytes, while this was suppressed by FLU. ABA-treated plants experienced much less oxidative damage measured as malondialdehyde and hydrogen peroxide contents. Exogenous application of proline, glycine betaine and trehalose (10 µM) also promoted the growth in heat-stressed plants and their action was not significantly affected with FLU application, suggesting that these osmolytes function downstream of ABA, mediating partially the protective effect of this hormone.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
34
Numer
5
Opis fizyczny
p.1651-1658,fig.,ref.
Twórcy
autor
  • Department of Botany, Panjab University, 160 014 Chandigarh, India
autor
  • Department of Botany, Panjab University, 160 014 Chandigarh, India
autor
  • Department of Botany, Panjab University, 160 014 Chandigarh, India
autor
  • Department of Botany, Panjab University, 160 014 Chandigarh, India
Bibliografia
  • Abass M, Rajashekar CB (1993) Abscisic acid accumulation in leaves and cultured cells during heat acclimation in grapes. HortSci 28:50–52
  • Allakhverdiev SI, Los DA, Mohanty P, Nishiyama Y, Murata N (2007) Glycinebetaine alleviates the inhibitory effect of moderate heat stress on the repair of photosystem II during photoinhibition. Biochim Biophys Acta 1767:1363–1371
  • Almeselmani M, Deshmukh PS, Sairam RK (2009) High temperature stress tolerance in wheat genotypes: role of antioxidant defence enzymes. Acta Agron Hung 57:1–4
  • Arnon DI (1949) Copper enzyme in isolated chloroplasts: polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15
  • Bates LS, Woldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–208
  • Brin M (1956) Transketolase: clinical aspects. Methods Enzymol 9:506–514
  • Camejo D, Torres W (2001) High temperature effect on tomato (Lycopersicon esculentum) pigment and protein content and cellular viability. Cultivos Trop 22:13–17
  • Chen THH, Murata N (2008) Glycinebetaine: an effective protectant against abiotic stress in plants. Trends Plant Sci 13:499–505
  • Coria NA, Sarquís JI, Peñalosa I, Urzúa M (1998) Heat-induced damage in potato (Solanum tuberosum) tubers: membrane stability, tissue viability, and accumulation of glycoalkaloids. J Agric Food Chem 46:4524–4528
  • Ding W, Song L, Wang X, Bi Y (2010) Effect of abscisic acid on heat stress tolerance in the calli from two ecotypes of Phragmites communis. Biol Plant 54:607–613
  • Fernandez O, Bethencourt L, Quero A, Sangwan RS, Clement C (2010) Trehalose and plant stress responses: friend or foe? Trends Plant Sci 15:409–417
  • Gao XP, Pan QH, Li MJ, Zhang LY, Wang XF, Shen YY, Lu YF, Chen SW, Liang Z, Zhang DP (2004) Abscisic acid is involved in the water stress-induced betaine accumulation in pear leaves. Plant Cell Physiol 45:742–750
  • Gong M, Li YJ, Chen SZ (1998) Abscisic acid-induced thermotolerance in maize seedlings is mediated by calcium and associated with antioxidant systems. J Plant Physiol 153:488–496
  • Grieve CM, Grattan SR (1983) Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil 70:303–307
  • Guo YP, Zhou HF, Zhang LC (2006) Photosynthetic characteristics and protective mechanisms against photooxidation during high temperature stress in two citrus species. Sci Hort 108:260–267
  • Halford NG (2009) New insights on the effects of heat stress on crops. J Exp Bot 60:4215–4216
  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
  • Howarth C (1989) Heat shock proteins in Sorghum bicolor and Pennisetum americanum: genotypic and developmental variation during seed germination. Plant Cell Environ 1:360–366
  • Kaplan F, Kopka J, Haskell DW, Zhao W, Cameron Schiller K, Gatzke N, Sung DY, Guy CL (2004) Exploring the temperaturestress metabolome of Arabidopsis. Plant Physiol 136:4159–4168
  • Karim MA, Fracheboud Y, Stamp P (2000) Effect of high temperature on seedling growth and photosynthesis of tropical maize genotypes. J Agron Crop Sci 184:217–223
  • Kaushal N, Gupta K, Bhandhari K, Kumar S, Thakur P, Nayyar H (2011) Proline induces heat tolerance in chickpea (Cicer arietinum L.) plants by protecting vital enzymes of carbon and antioxidative metabolism. Physiol Mol Biol Plants 17:203–213
  • Kumar S, Kaur G, Nayyar H (2008) Exogenous application of abscisic acid improves cold tolerance in chickpea (Cicer arietinum L.). J Agron Crop Sci 194:449–456
  • Kumar S, Kaur R, Kaur N, Bhandhari K, Kaushal N, Gupta K, Bains TS, Nayyar H (2011) Heat-stress induced inhibition in growth and chlorosis in mungbean (Phaseolus aureus Roxb.) is partly mitigated by ascorbic acid application and is related to reduction in oxidative stress. Acta Physiol Plant 33:2091–2101
  • Larkindale J, Knight MR (2002) Protection against heat stressinduced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol 128:682–695
  • Li S, Li F, Wang J, Zhang W, Meng Q, Chen THH, Murata N, Yang X (2011) Glycinebetaine enhances the tolerance of tomato plants to high temperature during germination of seeds and growth of seedlings. Plant Cell Environ 34:1931–1943
  • Liu X, Huang B (2000) Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Sci 40:503–510
  • Luo Y, Li F, Wang GP, Yang XH, Wang W (2010) Exogenouslysupplied trehalose protects thylakoid membranes of winter wheat from heat-induced damage. Biol Plant 54:495–501
  • Maestri E, Klueva N, Perrotta C, Gulli M, Nguyen T, Marmiroli N (2002) Molecular genetics of heat tolerance and heat shock proteins in cereals. J Plant Mol Biol 48:667–681
  • Mongrand S, Hare PD, Chua NH (2003) Abscisic Acid. Encyclopedia of hormones. Elsevier, London, pp 1–10
  • Mukherjee SP, Choudhuri MA (1983) Implications of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170
  • Nayyar H (2003) Accumulation of osmolytes and osmotic adjustment in water-stressed wheat (Triticum aestivum) and maize (Zea mays) as affected by calcium and its antagonists. Environ Exp Bot 50:253–264
  • Nayyar H, Gupta D (2006) Differential sensitivity of C₃ and C₄ plants to water deficit stress: association with oxidative stress and antioxidants. Environ Exp Bot 58:106–113
  • Nayyar H, Walia DP (2003) Water stress induced proline accumulation in contrasting wheat genotypes as affected by calcium and abscisic acid. Biol Plant 46:275–279
  • Nayyar H, Bains T, Kumar S (2005) Low temperature induced floral abortion in chickpea: relationship to abscisic acid and cryoprotectants in reproductive organs. Environ Exp Bot 53:39–47
  • Pareek A, Singla SL, Grover A (1998) Proteins alterations associated with salinity, desiccation, high and low temperature stresses and abscisic acid application in seedlings of Pusa 169, a highyielding rice (Oryza sativa L.) cultivar. Curr Sci 75:1023–1035
  • Premchandra GS, Sameoka H, Ogata S (1990) Cell osmotic membrane-stability, an indication of drought tolerance, as affected by applied nitrogen in soil. J Agric Res 115:63–66
  • Rojas A, Almoguera C, Jordano J (1999) Transcriptional activation of a heat shock gene promoter in sunflower embryos: synergism between ABI3 and heat shock factors. Plant J 20:601–610
  • Salvucci ME, Crafts-Brandner SJ (2004) Mechanism for deactivation of Rubisco under moderate heat stress. Physiol Plant 122:513–519
  • Shirasawa K, Takabe T, Takabe T, Kishitani S (2006) Accumulation of glycinebetaine in rice plants that overexpress choline monooxygenase from spinach and evaluation of their tolerance to abiotic stress. Ann Bot 98:565–571
  • Sohn SO, Back K (2007) Transgenic rice tolerant to high temperature with elevated contents of dienoic fatty acids. Biol Plant 51:340–342
  • Song SQ, Lei YB, Tian XR (2005) Proline metabolism and crosstolerance to salinity and heat stress in germinating wheat seeds. Russ J Plant Physiol 52:793–800
  • Song L, Ding W, Shen J, Zhang Z, Bi Y, Zhang L (2008) Nitric oxide mediates abscisic acid induced thermotolerance in the calluses from two ecotypes of reed under heat stress. Plant Sci 175:826–832
  • Steponkus PL, Lanphear FO (1967) Refinement of the triphenyl tetrazolium chloride method of determining cold injury. Plant Physiol 42:1423–1426
  • Suzuki N, Mittler R (2006) Reactive oxygen species and temperature stresses: a delicate balance between signaling and destruction. Physiol Plant 126:45–51
  • Takahashi S, Whitney S, Itoh S, Maruyama T, Badger M (2008) Heat stress causes inhibition of the de novo synthesis of antenna proteins and photobleaching in cultured Symbiodinium. PNAS 105:4203–4208
  • Trevelyan WE, Harrison JS (1956) Studies on yeast metabolism. 1. The trehalose content of baker’s yeast during the anaerobic fermentation. Biochem J 62:177–183
  • Verbruggen N, Hermans C (2008) Proline accumulation in plants: a review. Amino Acids 35:753–759
  • Wahid A, Gelani S, Ashraf M, Foolad MR (2007) Heat tolerance in plants: an overview. Environ Exp Bot 61:199–223
  • Wang WC, Nguyen HT (1989) Thermal stress evaluation of suspension cell cultures in winter wheat. Plant Cell Rep 8:108–111
  • Wang Z, Mamabelli S, Setter TL (2002) Abscisic acid catabolism in maize kernels in response to water deficit at early endosperm development. Ann Bot 90:623–630
  • Wang J, Gan YT, Clarke F, McDonald CL (2006) Response of chickpea yield to high temperature stress during reproductive development. Crop Sci 46:2171–2178
  • Waterland NL, Finer JJ, Jones ML (2010) Abscisic acid applications decrease stomatal conductance and delay wilting in droughtstressed chrysanthemums. Hort Tech 20:896–901
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