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1991 | 42 | 1 |
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Solcoseryl in prevention of stress-induced gastric lesions and healing of chronic ulcers

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Solcoseryl, a deproteinized extract of calf blood, protects the gastric mucosa against various topical irritants and enhances the healing of chronic gastric ulcerations but the mechanisms of these effects have been little studied. This study was designed to elucidate the active principle in Solcoseryl and to determine the role of prostaglandisn (PG) and polyamines in the antiulcer properties of this agent. Using both, the radioimmunoassay and radioreceptor assay, EGF-like material was detected in Solcoseryl preparation. Solcoseryl given s. c. prevented the formation of stress-indused gastric lesions and this was accompanied by an increase in the generation of PGE2 in the gastric mucosa. Similar effects were obtained with EGF. Pre treatment with indomethacin, to suppress mucosal generation of prostaglandins (PG), greatly augmented stress- induced gastric ulcerations and antagonized the protection exerted by both Solcoseryl and EGF. Solcoseryl, like EGF, enhanced the healing of chronic gastroduodenal ulcerations. This effect was abolished by the pretreatment with difluoro- methylomithine, an inhibitor of ornithine decarboxylas, the key enzyme in the biosynthesis of polyamines. The healing effects of Solcoseryl and EGF was also reduced by prednisolone which decreased the angiogenesis in the granulation tissue in the ulcer area. These results indicate that Solcoseryl 1. contains EGF-like material, 2. displays the protective and ulcer healing effects similar to those of EGF and involving both PG and polyamines and 3. acts via similar mechanism as does EGF.
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  • Institute of Physiology, University School of Medicine in Krakow, Grzegorzecka 16, 31-531Krakow, Poland
  • Institute of Physiology, University School of Medicine in Krakow, Grzegorzecka 16, 31-531Krakow, Poland
  • Institute of Physiology, University School of Medicine in Krakow, Grzegorzecka 16, 31-531Krakow, Poland
  • Institute of Physiology, University School of Medicine in Krakow, Grzegorzecka 16, 31-531Krakow, Poland
  • Institute of Physiology, University School of Medicine in Krakow, Grzegorzecka 16, 31-531Krakow, Poland
  • 1. Brzozowski T, Radecki T, Sendur R, Gustaw P, Konturek SJ. Prevention of acute gastric mucosal lesions by solcoseryl. Hepato-Gastroenteorology 1987; 34: 86-89.
  • 2. Kitajima M, Miyake J, Matsui K, Ogawa Y, Sohma S. Etiology of stress-induced ulcers and pharmacological effects of solcoseryl. In: Miyake T (ed) Cytoprotection and Cytobiology: Excerpta Medica, Tokyo, 1984; 33-37.
  • 3. Konturek SJ, Brzozowski T, Dembiński A, Warzecha Z, Drozdowicz D. Comparison of solcoseryl and epidermal growth factor (EGF) in healing of chronoc gastroduodenal ulcerations and mucosal growth in rats. Hepato-Gastroenterology 1988; 35: 25-29.
  • 4. Yamagata S. Clinical efficacy of solcoseryl in peptic ulcer. J New Rem Clin 1965; 14: 1183- 1189.
  • 5. Pawlik WW. Gustaw P, Sendur R, Czamobilski K, Konturek SJ. Contribution of prostanoids to gastric circulatory and metabolic actions of solcoseryl. Pol J Pharmacol Pharm 1990 ; 42 (in press).
  • 6. Arai I, Muramatsu M, Aihara H. Body temperature dependency of gastric regional blood flow, acid secretion and ulcer formation in restraint and water-immersed rats. Jpn J Pharmacol 1986; 40: 501-504.
  • 7. Kitagiwa A, Fujinara M, Osumi Y. Effects of water-immersion stress on gastric secretion and mucosal blood flow in rats. Gastroenterology 1979; 77: 298-302.
  • 8. Konturek SJ, Dembiński A, Brzozowski T, Drozdowicz D, Konturek PK. Role of epidermal growth factor (EGF), prostaglandins and sulfhydryls in stress-induced gastric leisons. Gastroenterology 1990; 99: 1607-1615.
  • 9. Basso N, Materia A, Forlini A, Jaffe BM. Prostaglandin generation in gastric mucosa of rats with stress ulcers. Surgery 1983; 94: 104-118.
  • 10. Rudowski W, Klucinski W, Kopic et al. Effect of protein-free hemodialysate on tissue respiration and healing of bums. Bums 1984; 10: 363-367.
  • 11. Kern H, Obrousky L. Die Behandlung trophischer Hautschaden mit Solcoseryl. Ost Mh Arztl Forbild. 1965; 6: 3-17.
  • 12. Gaebel E, Paul O. Uber die Therapie der Rontgenulccra mit Solcoseryl. Munch Medschr 1961; 103: 1377-1379.
  • 13. Delachaux A, Calana. Experimentation clinique avec the Solcoseryl dans le traitment des escarres de decubitus chez des malades avec ages et chronique. Praxis 1963 ; 54: 1587-1589.
  • 14. Niinikoski J, Renvall S, Laato M, Tschannen R, Fraefel W. Effect of a hexosylceramide fraction of the homodialysate Solcoseryl on experimental granulation tissue. Eur Surg Res 1986; 18: 58-64.
  • 15. Rohr HP. The effect of Solcoseryl treatment on angiogensis and reepithelization of bum wounds in rats. Buras (in press).
  • 16. Niinikoski J, Laato M, Tschannen R, Fraefel W. Effect of hexosylceramide fraction of the hemodialysate Solcoseryl on wound healing angiogenesis. Eur Surg Res 1984 (Suppl 16), 51, 106-107.
  • 17. Fulop E. An investigation with enzyme histochemical methods of the effects of solcoseryl on epithelization. Orn Helit 1969; 10: 126-129.
  • 18. Dijke P, Iwata KK. Growth factors for wound healing. Bio/Technology 1989; 7: 793-798.
  • 19. Russel DH, Durie BG. Ornithine decarboxylase - a key enzyme in growth. Proc Congr Res Thor 1978; 8: 43-58.
  • 20. Pegg AE. Recent advances in the biochemistry of polyamines in eukaryotes. Bichern J. 1986; 234 : 249-262.
  • 21. Konturek SJ, Pawlik WW, Mysh W et al. Comparison of organ uptake and disappearance half-time of human epidermal growth factor and insulin. Reg Pept 1990; 30: 137-148.
  • 22. Imai Y, Tsushima T, Sasaki N, Matsuzuki F. Radioreceptor assay for epidermal growth factor. In: Shimize K,Takano K (eds). Growth and Growth factors. Univ Tokyo Press; Tokyo, 1979; 275-288.
  • 23. Yip TT, Tam YY, Keung WM, Xin JX, Kong YC. Studies on shrew (Suncus murinus) epidermal growth factor. Acta Endocrinol 1986; 11: 423-432.
  • 24. Konturek SJ, Piastucki I, Brzozowski T, Radecki T, Dembinska-Kiec A, Gryglewski R. Roleof prostaglandins in the formation of aspirin-induced gastric ulcers. Gastroenterology 1981; 80: 4-10.
  • 25. Hase S. Nakazawa S, Tsukamoto Y, Segawa K. Effects of prednisolone and epidermal growth factor on angiogenesis in granulation tissue of gastric ulcer induced by acetic acid. Digestion 1989; 42: 135-142.
  • 26. Kimura M, Amemiya K, Yamada T. Quantitative methods for measuring adjuvant-induced granuloma angiogenesis in insulin-treated diabetic mice. J. Pharmacobiodyn 1986; 9: 442- 446.
  • 27. Takeuchi K, Okabe S, Takagi K. A new model of stress ulcers in rats with pylorus ligation and its pathogenesis. Am J Dig Dis 1977; 21: 742-788.
  • 28. Okabe S, Pfeiffer CJ, Roth JLA. A method for experimental penetrating gastric and duodenal ulcers in rats. Am J Dig Dis 1971; 16: 277-284.
  • 29. Lichtenberger LM, Graziani LA, Dial EJ, Butler BD, Hills BA. Role of surface-active phospholipids in gastric cytoprotection. Science 1983; 219: 1327-1329.
  • 30. Hills BA, Butler BD, Lichtenberger LM. Gastric mucosal barrier: hydrophobic lining to the lumen of the stomach. Am J Physiol 1983; 244: G561-568.
  • 31. Metcalf BW, Mey P. Catalytic irreversible inhibition of mammalian ornithine decarboxylase by substrate and product analogues. J Am Chem Soc 1978; 100, 2551-2553.
  • 32. Seidler N. Polyamine metabolism. Digestion 1990; 46 (suppl 2): 319-330.
  • 33. Wang J-Y, Johnson LR. Luminal polyamines stimulate repair of gastric mucosal stress ulcers. Am J Physiol 1990; 259: G584-592.
  • 34. Kozma SC, Ferrari S, Thomas G. Unmasking a growth factor/oncogene activated S6-phosphorilation cascade. Cell Sign 1989; 1: 219-225.
  • 35. Baschong W, Bauen A, Isler H, Huggel K, Imber R, Fabbro D. The lipidic part of a homodialysate used for treatment of poorly healing wounds activates S6-kinase. J Cell Biochem (Suppl) 1990; 14E: 250.
  • 36. Peacock EE JR. Wound Healing. WB Saunders; Philadelphia. 1984, 307-330.
  • 37. Hunt TK, Zederfeldt B, Goldstrick TK. Oxygen and healing. Am J Surg 1969; 118: 521-525.
  • 38. Hansson HA, Hong L, Helander HF. Changes in gastric EGF, EGF receptors and acidity during healing of gastric ulcer in the rat. Acta Physiol Scand 1990; 138: 241-242.
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