PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 17 | 1 |
Tytuł artykułu

Changes in Th1 and Th2 cytokine concentrations in ileal Peyer’s patches in gilts exposed to zearalenone

Autorzy
Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
GALT induces tolerance to foreign food antigens and plays an important role in the development of food allergies and the inflammatory bowel disease. The immune function of GALT is significantly influenced by an equilibrium between Th1 and Th2 subpopulations and the cytokines they produce. Th1 cytokines participate in the induction of a cell-mediated immune response, whereas Th2 cytokines induce powerful antibody-mediated responses. Changes in Th1/Th2 cell polarization of an immune response are associated with susceptibility to autoimmune and infectious diseases. This experiment investigated changes in cytokine levels produced by Th1 and Th2 cells in ileal Payer’s patches in gilts exposed to ZEN doses below the NOEL (approximately 8 μg kg⁻¹ BW) for 14, 28 and 42 days. A significant linear increase in IL-4 (40.32 ± 1.55 ng mg⁻¹ - 137.60 ± 29.96 ng mg⁻¹), and IL-10 (5.99 ± 0.15 ng mg⁻¹ - 16.39 ± 1.11 ng mg⁻¹) concentrations was observed. An increase in Th1 (IL-2 and IFN-γ) cytokine levels was also noted in the experimental group, but it was not statistically significant. An HPLC analysis of Peyer’s patches in group E animals revealed a linear increase in ZEN concentrations (3.65 ± 0.91 ng g⁻¹ - 4.72 ± 1.85 ng g⁻¹) and an absence of α-ZEL. IL-4 stimulates monocytes and macrophages, it induces the production of proinflammatory cytokines and it may directly and indirectly contribute to the development of inflammatory foci. Higher IL-4 levels could shift polarization toward Th2 cells, stimulate B cells to undergo class switching to produce IgE and contribute to the development of allergies.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
17
Numer
1
Opis fizyczny
p.53-59,fig.,ref.
Twórcy
autor
  • Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
Bibliografia
  • Agnello D, Lankford CS, Bream J, Morinobu A, Gadina M, O’Shea JJ, Frucht DM (2003) Cytokines and transcription factors that regulate T helper cell differentiation: new players and new insights. J Clin Immunol 23: 147-161.
  • Barker DJ (2004) Developmental origins of adult health and disease. J Epidemiol Community Health 58: 114-115.
  • Bennett JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16: 497-516.
  • Biehl ML, Prelusky DB, Koritz GD, Hartin KE, Buck WB, Trenholm HL (1993) Biliary excretion and enterohepatic cycling of zearalenone in immature pigs. Toxicol Appl Pharmacol 121: 152-159.
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
  • Brandtzaeg P, Pabst R (2004) Let’s go mucosal: communication on slippery ground. Trends Immunol 25: 570-577.
  • Chen J, Liu X (2009) The role of interferon gamma in regulation of CD4+ T-cells and its clinical implications. Cell Immunol 254: 85-90.
  • Chighizola C, Meroni PL (2012) The role of environmental estrogens and autoimmunity. Autoimmun Rev 11: A493-A501.
  • Coffey DS (2001) Similarities of prostate and breast cancer: evolution, diet, and estrogens. Urology 57 (Suppl 1): 31-38.
  • Crawley A, Raymond C, Wilkie BN (2003) Control of immunoglobulin isotype production by porcine B-cells cultured with cytokines. Vet Immunol Immunopathol 91: 141-154.
  • Decasto M, Rolando P, Nachtmann C, Ceppa L, Nebbia C (1995) Zearalenone mycotoxicosis in piglets suckling sows fed contaminated grain. Vet Hum Toxicol 37: 359-361.
  • Delpy L, Douin-Echinard V, Garidou L, Bruand C, Saoudi A, Guery JC (2005) Estrogen enhances susceptibility to experimental autoimmune myasthenia gravis by promoting type 1-polarized immune responses. J Immunol 175: 5050-5057.
  • EC (European Commission) Recommendation (2006) Commission recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding. Off J Eur Union L229, 7-9 (23.8.2006).
  • EFSA (European Food Safety Authority) (2011) Opinion on the risks for public health related to the presence of zearalenone in food. EFSA J 9: 1-124.
  • Faas MM, Bakker N, Valkhof N, Schuiling GA (1999) Effect of estradiol and progesterone on the low-dose endotoxin- induced glomerular inflammatory response of the female rat. Am J Reprod Immunol 41: 224-231.
  • Finkelman FD, Holmes J, Katona IM, Urban JF, Beckmann MP, Park LS, Schooley KA, Coffman RL, Mosmann TR, Paul WE (1990) Lymphokine control of in vivo immunoglobulin isotype selection. Annu Rev Immunol 8: 303-333.
  • Gajęcki M (2002) Zearalenone - undesirable substances in feed. Pol J Vet Sci 5: 117-122.
  • Jensen PT, Christensen K (1981) Genetic studies on the in vitro PHA-transformation of porcine blood lymphocytes. Vet Immunol Immunopathol 2: 133-143.
  • Malekinejad H, Maas-Bakker R, Fink-Gremmels J (2006) Species differences in the hepatic biotransformation of zearalenone. Vet J 172: 96-102.
  • Maret A, Coudert JD, Garidou L, Foucras G, Gourdy P, Krust A, Dupont S, Chambon P, Druet P, Bayard F, Guery JC (2003) Estradiol enhances primary antigen-specific CD4 T cell responses and Th1 development in vivo. Essential role of estrogen receptor alpha expression in hematopoietic cells. Eur J Immunol 33: 512-521.
  • Marin DE, Taranu I, Burlacu R, Manda G, Motiu M, Neagoe I, Dragomir C, Stancu M, Calin L (2011) Effects of zearalenone and its derivatives on porcine immune response. Toxicol In Vitro 25: 1981-1988.
  • Mouton D, Sant’Anna OA, Biozzi G (1988) Multigenic control of specific and non-specific immunity in mice. A review. Livest Prod Sci 20: 277-286.
  • Mowat AM, Viney JL (1997) The anatomical basis of intestinal immunity. Immunol Rev 156: 145-166.
  • Obremski K, Gajęcki M, Zwierzchowski W, Bakuła T, Apoznaniski J, Wojciechowski J (2003a) The level of zearalenone and α-zearalenol in the blood of gilts with clinical symptoms of toxicosis, fed diets with a low zearalenone content. J Anim Feed Sci 12: 529-538.
  • Obremski K, Gajęcki M, Zwierzchowski W, Zielonka Ł, Otrocka- Domagała I, Rotkiewicz T, Mikołajczyk A, Gajęcka M, Polak M (2003b) Influence of zearalenone on reproductive system cell proliferation in gilts. Pol J Vet Sci 6: 239-245.
  • Polanczyk MJ, Carson BD, Subramanian S, Afentoulis M, Vandenbark AA, Ziegler SF, Offner H (2004) Cutting edge: estrogen drives expansion of the CD4+CD25+ regulatory T cell compartment. J Immunol 173: 2227-2230.
  • Salem ML (2004) Estrogen, a double-edged sword: modulation of TH1- and TH2-mediated inflammations by differential regulation of TH1/TH2 cytokine production. Curr Drug Targets Inflamm Allergy 3: 97-104.
  • Sattar Ansar A (2000) The immune system as a potential target for environmental estrogens (endocrine disrupters): a new emerging field. Toxicology 150: 191-206.
  • Strobel S, Mowat AM (1998) Immune responses to dietary antigens: oral tolerance. Immunol Today 19: 173-181.
  • Sundstedt A, O’Neill EJ, Nicolson KS, Wraith DC (2003) Role for IL-10 in suppression mediated by peptide-induced regulatory T cells in vivo. J Immunol 170: 1240-1248.
  • Swain SL, Bradley LM, Croft M, Tonkonogy S, Atkins G, Weinberg AD, Duncan DD, Hedrick SM, Dutton RW, Huston G (1991) Helper T-cell subsets: phenotype, function and the role of lymphokines in regulating their development. Immunol Rev 123: 115-144.
  • Straub RH (2007) The complex role of estrogens in inflammation. Endocr Rev 28: 521-574.
  • Tiemann U, Brüssow KP, Küchenmeister U, Jonas L, Pöhland R, Reischauer A, Jäger K, Dänicke S (2008) Changes in the spleen and liver of pregnant sows and full-term piglets after feeding diets naturally contaminated with deoxynivalenol and zearalenone. Vet J 176: 188-196.
  • Vazquez E, Gil A, Garciá-Olivares E, Rueda R (2000) Weaning induces an increase in the number of specific cytokine-secreting intestinal lymphocytes in mice. Cytokine 12: 1267-1270.
  • Vlata Z, Porichis F, Tzanakakis G, Tsatsakis A, Krambovitis E (2006) A study of zearalenone cytotoxicity on human peripheral blood mononuclear cells. Toxicol Lett 165: 274-281.
  • Zinedine A, Soriano JM, Moltó JC, Mañes J (2007) Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: an oestrogenic mycotoxin. Food Chem Toxicol 45: 1-18.
  • Zwierzchowski W, Przybyłowicz M, Obremski K, Zielonka Ł, Skorska-Wyszyńska E, Gajęcka M, Polak M, Jakimiuk E, Jana B, Rybarczyk L, Gajęcki M (2005) Level of zearalenone in blood serum and lesions in ovarian follicles of sexually immature gilts in the course of zearalenone mycotoxicosis. Pol J Vet Sci 8: 209-218.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-e019289a-af30-4fdf-9120-f05e2ca9e634
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.