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2018 | 74 | 06 |
Tytuł artykułu

Wpływ kwasu glutaminowego na uwalnianie katecholamin z podwzgórza królika w badaniach ex vivo

Warianty tytułu
EN
Glutamate influence on catecholamine release from rabbits hypothalamus - ex vivo
Języki publikacji
PL
Abstrakty
EN
The aim of the study was to find the direct effects of a possible correlation between different glutamate concentrations (5, 50 and 200 µM) on the one hand and catecholamine release from rabbit hypothalamus homogenate during a 90 min incubation on the other hand. Brain structures were obtained from 12 decapitated female rabbits that were 12 weeks old. After decapitation the obtained brain of the animal was placed in 0.9% NaCl. Next, each brain structure from each rabbit (approx. 50 mg) was placed in incubation wells (cell culture Sigma) containing 1 ml of incubation medium (Krebs phosphate buffer containing glucose 0.3% bovine serum albumin and 0.1% BSA) without (control) or with three doses of Glu in a concentration of the following: I – 5 μM, II – 50 μM, and III – 200 μM. Every 30 minutes each slice of tissue of each brain structure was placed in the next hole with the medium. The medium collected after 30, 60 and 90 minutes of the experiment was used for dopamine, noradrenaline and adrenaline RIA measurement. The obtained results show that Glu has a direct influence on catecholamines release from the hypothalamus as a central brain structure of the HPA axis in stress inducing and adaptive processes. Generally, there was an inhibitory effect on catecholamine release from the hypothalamus homogenates. Our findings show that Glu has great influence on the brain’s motivational structure, which may indicate its contribution to stress response by modulating the amount of catecholamine released.
Wydawca
-
Rocznik
Tom
74
Numer
06
Opis fizyczny
s.401-404,rys.,bibliogr.
Twórcy
autor
  • Katedra Fizjologii i Endokrynologii Zwierząt, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Rolniczy im. Hugona Kołłątaja, Al.Mickiewicza 24/28, 30-059 Kraków
autor
  • Instytut Weterynarii, Uniwersyteckie Centrum Medycyny Weterynaryjnej UJ-UR, Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie, Al.Mickiewicza 24/28, 30-059 Kraków
autor
  • Katedra Genetyki i Metod Doskonalenia Zwierząt, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Rolniczy im. Hugona Kołłątaja, Al.Mickiewicza 24/28, 30-059 Kraków
autor
  • Katedra Genetyki i Metod Doskonalenia Zwierząt, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Rolniczy im. Hugona Kołłątaja, Al.Mickiewicza 24/28, 30-059 Kraków
autor
  • Katedra Genetyki i Metod Doskonalenia Zwierząt, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Rolniczy im. Hugona Kołłątaja, Al.Mickiewicza 24/28, 30-059 Kraków
Bibliografia
  • Dawson M. E., Neuchterlein K. H., Shell A. M., Gitlin M., Ventura J.: Autonomic abnormalities in schizophrenia: State or trait indicators? Arch. Gen. Psychiatry 1994, 51, 813-824.
  • Douglas R. J., Martin K. A.: Mapping the matrix: the ways of neocortex. Neuron 2007, 56, 226-238.
  • Fedder K. N., Sbo S. L.: On the role of glutamate in presynaptic development: possible contributions of presynaptic BMDA receptors. Biomolecules 2015, 5, 3448-3466.
  • Garcia A. G., Garcia-de-Diego A. M., Gandia L., Borges R., Garcia-Sancho J.: Calcium signaling and exocytoosis in adrenal chromaffin cells. Physiol. Rev. 2006, 86, 1093-1131.
  • Guilemin R.: Hypothalamic hormone a.k.a. hypothalamic releasing factors. J. Endocrinol. 2005, 184, 11-286.
  • Holzbauer M., Sharman D. F.: Distribution of catecholamines in vertebrates, [w:] Blaschko H., Muscholl I. E. (red.): Catecholamines: Handbook of Experimental Pharmacology. Berlin, Springer 1972, 33, 110-185.
  • Huang W. T., Niu K. C., Chang C. K., Lin M. T., Chang C. P.: Curcumin inhibit the increase of glutamate, hydroxyl radicals and PGE2 in the hypothalamus and reduces fever during LPS-induced systemic inflammation in rabbits. Eur. J. Pharmacol. 2008, 593, 105-111.
  • Humphries P., Pretorius P. E., Naudé H.: Direct and indirect cellular effects of aspartame. Eur. J. Clin. Nutr. 62, 451-462.
  • Okada Y., Nitsch-Hassler C., Kim J. S., Bak I. J., Hassler R.: Role of γ-aminobutyric acid (GABA) in the extrapyramidal motor system. 1. Regionnal distribution of GABA in rabbit, rat, guinea pig and baboon CNS. Exp. Brain Res. 1991, 13, 514-515.
  • Orrego F., Villaneuva S.: The chemical nature of the main central excitatory transmitter: A critical appraisal based upon release studies and synaptic vesicle localization. Neurosci. 1993, 56, 539-555.
  • Pralong E., Magistretti P., Stoop R.: Cellular perspectives on the glutamatemonoamine interactions in limbic lobe structures and their relevance for some psychiatric disorders. Progr. Neurobiol. 2002, 67, 173-202.
  • Rosa J. M., Nanclares C., Orozco A., Colmena J., Pascual R., de, Garcia A. G., Gansia L.: Regulation by L-type calcium channels of endocytosis: An overview. J. Mol. Neurosci. 2012, 48, 360-367.
  • Sanacora G., Treccani G., Popoli M.: Towards a glutamate hypothesis of depression: An emerging frontier of neuropsychopharmacology for mood disorders. Neuropharmacol. 2012, 62, 63-77.
  • Tong Q., Ye C. P., McCrimmon R. J., Dhillon H., Choi B., Kramer M. D., Yu J., Lee Z. C. E., Choi C. S., Zigman J. M., Shalman G. J., Sherwin R. S., Elmquist J. K., Lowell B. B.: Synaptic glutamate release by ventromedial hypothalamic Neurons part of hypoglycemiathe neurocircuitry that prevents. Cell Metab. 2007, 5, 383-393.
  • Welker W., Johnson J. I., Noe A.: Comparative mammalian brain collection. Univ. of Wisconsin, Michigan State Univ., USA 2001.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-dfb99900-5867-4d06-8971-cc3c7328d68a
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