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2017 | 73 | 06 |
Tytuł artykułu

Determination of Neospora caninum and Toxoplasma gondii in aborted bovine foetuses by duplex PCR, immunohistochemistry and immunofluorescence methods

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
This study was aimed at determining the existence of Neospora caninum and Toxoplasma gondii in aborted bovine foetuses. In this research, 102 bovine foetuses were examined by duplex PCR, immunohistochemistry and immunofluorescence to determine the presence of N. caninum and T. gondii. None of the aborted bovine foetuses were shown to have T. gondii, but N. caninum was detected in 26 foetuses (25.49%) by duplex PCR, in 18 (17.64%) by immunohistochemistry and in 8 (7.84%) by immunofluorescence. Moreover, 16 livers, 13 kidneys, 12 spleens, 8 thymuses and 5 brains of the 18 foetuses examined by immunohistochemistry showed immunopositivity. Positive staining was found in the spleen of eight foetuses by the immunofluorescence method. In this study, immunohistochemistry was shown to be superior to immunofluorescence in terms of diagnosis. Although immunofluorescence has the advantage of being the easiest to use in practice, it makes diagnosis more difficult because of its non-specific staining.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
73
Numer
06
Opis fizyczny
p.345-351,fig.,ref.
Twórcy
autor
  • Department of Pathology, Faculty of Veterinary Medicine, Atatürk University, 25240 Erzurum, Turkey
autor
  • Veterinary Control Institute, Elazıg, Turkey
autor
  • Veterinary Control Institute, Istanbul, Turkey
autor
  • Veterinary Control Institute, Elazıg, Turkey
autor
  • Department of Pathology, Faculty of Veterinary Medicine, Atatürk University, 25240 Erzurum, Turkey
Bibliografia
  • Alkan Z., Özbel Y., Özensoy S., Atambay M.: Moleküler Biyolojik Yöntemler, [in:] Özcel M. A., Altindaş N. (Ed): Parazit Hastalıklarında Tanı, Türkiye Parazitol. Dern. İzmir 1997, p. 15.
  • Amin A. M., Morsy T. A.: Anti-Toxoplasma antibodies in butchers and slaughtered sheep and goats in Jeddah Municipal abattoir, Saudi Arabia. J. Egypt. Soc. Parasitol. 1997, 27, 913-918.
  • Anderson M. L., Andrianarivo A. G., Conrad P. A.: Neosporosis in cattle. Anim. Reprod. Sci. 2000, 417-431.
  • Anderson M. L., Blanchard P. C., Barr B. C., Dubey J. P., Hoffman R. L., Conrad P. A.: Neospora-like protozoan infection as a major cause of abortion in California dairy cattle. J. Am. Vet. Med. Assoc. 1991, 198, 241-244.
  • Barr B. C., Anderson M. L., Blanchard P. C., Daft B. M., Kinde H., Conrad P. A.: Bovine fetal encephalitis and myocarditis associated with protozoal infections. Vet. Pathol. 1990, 27, 354-361.
  • Bekele T., Kasali O. B.: Toxoplasmosis in sheep, goats and cattle in central Ethiopia. Vet. Res. Commun. 1989, 13, 371-375.
  • Bowman D. D., Lynn R. C., Eberhard M. L.: Georgis’ Parasitology for Veterinarians. Elsevier, Science 2003.
  • Buxton D.: Protozoan infections (Toxoplasma gondii, Neospora caninum and Sarcocystis spp.) in sheep and goats: recent advances. Vet. Res. 1998, 29, 289-310.
  • Cabaj W., Choromański L., Rodgers S., Moskwa B., Malczewski A.: Neospora caninum infections in aborting dairy cows in Poland. Acta Parasitol. 2000, 45, 113-114.
  • Cabral A. D., Camargo C. N., Galleti N. T. C., Okuda L. H., Pituco E. M., Del Fava C.: Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR. Rev. Bras. Parasitol. Vet. 2009, 18, 4.
  • Cabral A. D., Camargo C. N., Galleti N. T. C., Okuda L. H., Pituco E. M., Del Fava C.: Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil. Arq. Inst. Biol. 2013, 80, 103-105.
  • Canada N., Meireles C. S., Rocha A., Correia Da Costa J. M., Erickson M. W., Dubey J. P.: Isolation of viable Toxoplasma gondii from naturally infected aborted bovine fetuses. J. Parasitol. 2002, 88, 1247-1248.
  • Chabra M. B., Gupta S. L., Gautam O. P.: Toxoplasma seroprevalance in animals in Northern India. Int. J. Zoonoses. 1985, 12, 136-142.
  • Davison H. C., Otter A., Trees A. J.: Significance of Neospora caninum in British dairy cattle determined by estimation of seroprevalence in normally calving cattle and aborting cattle. Int. J. Parasitol. 1999, 29, 1189-1194.
  • Dubey J. P.: Recent advances in Neospora and neosporosis. Vet. Parasitol. 1999, 84, 349-367.
  • Dubey J. P.: Review of Neospora caninum and neosporosis in animals. Korean J. Parasitol. 2003, 41, 1-16.
  • Dubey J. P., Barr B. C., Barta J. R., Bjerkas I., Björkman C., Blagburn B. L., Bowman D., Buxton D., Ellis J. T., Gottstein B., Hemphill A., Hill D. E., Howe D. K., Jenkins M. C., Kobayaski Y., Koudela B., Marsh A. E., Mattsson J. G., McAllister M. M., Modry D., Omata Y., Sibley L. D., Speer C. A., Trees A. J., Uggla A., Upton S. J., Williams D. J. L., Lindsay D. S.: Redescription of Neospora caninum and its differentiation from related coccidian. Int. J. Parasitol. 2002, 32, 929-946.
  • Dubey J. P., Beattie C. P.: Toxoplasmosis of Animal and Man. CRC Press 1988, p. 61-80.
  • Dubey J. P., Buxton D., Wouda W.: Pathogenesis of bovine neosporosis. J. Comp. Pathol. 2006, 134, 267-289.
  • Dubey J. P., Dorough K. R., Jenkins M. C., Liddell S., Speer C. A., Kwok O. C. H., Shen S. K.: Canine neosporosis clinical signs, diagnosis, treatment, treatment and isolation of Neospora caninum in mice and cell culture. Int. J. Parasitol. 1998, 28, 1293-1304.
  • Dubey J. P., Lindsay D. S.: A review of Neospora caninum and neosporosis Vet. Parasitol. 1996, 67, 1-59.
  • Dubey J. P., Schares G.: Diagnosis of bovine neosporosis. Vet. Parasitol. 2006, 140, 1-2.
  • Dumanli N., Aktaş M.: Toxoplasmatidae (Toxoplasma, Neospora), [in:] Dumanli N., Karaer Z. (ed.): Veteriner Protozooloji. 2010, p. 119-136.
  • Georgieva D. A., Prelezov P. N., Koinarski V. T. S.: Neospora caninum and neosporosis in animals – A review. Bulgarian J. Vet. Med. 2006, 9, 1-26.
  • Hemphill A.: The host-parasite relationship in neosporosis. Adv. Parasitol. 1999, 43, 47-104.
  • Kaufmann J.: Parasitic infections of domestic animals. A diagnostic manual. Birkhause Verlag 1996, p. 423.
  • Kul O.: Epidemiology and Pathogenesis of Neospora caninum Infection: Special Emphasis to Neosporosis Status of Turkey. Animal Health, Production and Hygiene 2012, 1, 70-79.
  • Levine N. D.: Veterinary Protozoology. Iowa State University Press 1985, p. 413.
  • Lindsay D. S., Dubey J. P.: Immunohistochemical diagnosis of Neospora caninum in tissue sections. Am. J. Vet. Res. 1989, 50, 1981-1983.
  • Lindsay D. S., Dubey J. P., McAllister M.: Neospora caninum and the potential for parasite transmission. Small Animal. 1999, 21, 317-320.
  • Masala G., Porcu R., Madau L., Tanda A., Ibba B., Sata G., Tola S.: Survey of ovine and caprine Toxoplasmosis by IFAT and PCR assays in Sardina, Italy. Vet. Parasitol. 2003, 117, 15-21.
  • McAllister M. M., Dubey J. P., Lindsay D. S., Jolley W. R., Wills R. A., McGuire A. M.: Dogs are definitive hosts of Neospora caninum. Int. J. Parasitol. 1998, 28, 1473-1478.
  • Medina L., Cruz-Vázquez C., Quezada T., Morales E., García-Vázquez Z.: Survey of Neospora caninum infection by nested PCR in aborted fetuses from dairy farms in Aquascalientes, Mexico. Vet. Parasitol. 2006, 136, 187-191.
  • Moore D. P., Regidor-Cerrillo J., Morrell E., Poso M. A., Cano D. B., Leunda M. R., Linschinky L., Odeón A. C., Odriozola E., Ortega-Mora L. M., Campero C. M.: The role of Neospora caninum and Toxoplasma gondii in spontaneous bovine abortion in Argentina. Vet. Parasitol. 2008, 156, 163-167.
  • Morales E., Trigo F. J., Ibarra F., Puente E., Santa Cruz M.: Neosporosis in Mexican Dairy Herds: Lesions and Immunohistochemical Detection of Neospora caninum in Fetuses. J. Comp. Pathol. 2001, 125, 58-63.
  • Naguleswaran A., Hemphill A., Rajapakse R. P. V. J., Sager H.: Elaboration of a crude antigen ELISA for serodiagnosis of caprine Neosporosis: validation of the test by detection of Neospora caninum-specific antibodies in goats from Sri Lanka. Vet. Parasitol. 2004, 126, 257-262.
  • O’Donoghue J. P., Riley M. J., Clarke J. F.: Serological survey for Toxoplasma infections in sheep. Aust. Vet. J. 1987, 64, 40-45.
  • Odriozola H., Bretschneider G., Poso M. A.: Neospora caninum associated abortion in a dairy herd in Argentina. Vet. Rec. 1998, 143, 228-229.
  • Pescador C. A., Corbellini L. G., Oliveira E. C., Raymundo D. L., Driemeier D.: Histopathological and immunohistochemical aspects of Neospora caninum diagnosis in bovine aborted foetuses. Vet. Parasitol. 2007, 150, 159-163.
  • Quintanilla-Gozalo A., Pereira-Bueno J., Tabares E., Innes E. A., Gonzalez-Paniello R., Ortega-Mora L. M.: Seroprevalence of Neospora caninum infection in dairy and beef cattle in Spain. Int. J. Parasitol. 1999, 29, 1201-1208.
  • Paştiu O. Ş. A., Adriana Györke A., Cozma V.: Molecular detection of Neospora caninum abortion in dairy cattle from different historical regions of Romania. Sci. Parasitol. 2012, 13, 159-162.
  • Reitt K., Hilbe M., Voegtlin A., Corboz L., Haessig M., Pospischil A.: Aetiology of Bovine Abortion in Switzerland from 1986 to 1995 – A Retrospective Study with Emphasis on Detection of Neospora caninum and Toxoplasma gondii by PCR. J. Vet. Med. 2007, 54, 15-22.
  • Rommel M.: Protozoenifektionen der Wiederkaufer, [in:] Rommel M., Eckert J., Kutzer E., Körting W., Schneider T. (eds.): Veterinärmedizinische Parasitologie 5. Blackwell Wissenschafts-Verlag 2001, p. 121-191.
  • Sager H., Fischer I., Furrer K., Strasser M., Waldvogel A., Boerlin P., Audigé L., Gottstein B. A.: Swiss case-control study to assess Neospora caninum-associated bovine abortions by PCR, histopathology and serology. Vet. Parasitol. 2001, 102, 1-15.
  • Soulsby E. L. J.: Helminths. Arthropods and Protozoa of Domesticated Animals. Bailliere Tindall 1986, p. 809.
  • Tenter A. M., Heckeroth A. R., Weiss L. M.: Toxoplasma gondii: from animals to humans. Int. J. Parasitol. 2000, 30, 1217-1258.
  • Tramuta C., Lacerenza D., Zoppi S., Goria M., Dondo A., Ferroglio E., Nebbia P., Rosati S.: Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples. J. Vet. Diagn. Invest. 2011, 23, 657-664.
  • Vazquez Z. G., Vazquez C. C., Espinosa L. M., Tapia D. G., Martinez B. C.: Serological survey of Neospora caninum infection in dairy cattle herds in Aquascalientes, Mexico. Vet. Parasitol. 2002, 106, 115-120.
  • Wouda W., Moen A. R., Visser I. J. R., Van Knapen F.: Bovine fetal neosporosis: a comparison of epizootic and sporadic abortion cases and different age classes with regard to lesion severity and immunohistochemical identification of organisms in brain, heart, and liver. J. Vet. Diagn. Invest. 1997, 9, 180-185.
  • Zhai Y. Q., Zhao J. P., Zhu X. Q., Li L., Wrang C. R.: Research advances in the diagnosis of cattle neosporosis. J. Anim. Vet. Adv. 2007, 6, 1377-1387.
Typ dokumentu
Bibliografia
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