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2018 | 67 | 1 |

Tytuł artykułu

Rapid detection of bloodstream pathogens in oncologic patients with a FilmArray multiplex PCR assay: comparison with culture methods

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The results of the FilmArray® Blood Culture Identification Panel (BCID) (BioFire Diagnostics) and the culture with susceptibility testing of 70 positive blood cultures from oncologic patients were compared. The multiplex PCR assay (BCID) identified 81 of the 83 isolates (97.6%), covered by the panel. The panel produced results in significantly shorter time than standard identification methods, when counted from receiving positive blood cultures bottles to the final results. It is an accurate method for the rapid identification of pathogens and resistance genes from blood culture in oncologic patients.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

67

Numer

1

Opis fizyczny

p.103-107,ref.

Twórcy

  • Professor Franciszek Lukaszczyk Oncology Center in Bydgoszcz, Microbiology Department, Bydgoszcz, Poland
  • Professor Franciszek Lukaszczyk Oncology Center in Bydgoszcz, Microbiology Department, Bydgoszcz, Poland

Bibliografia

  • Alkhamis T., D. Ivić, J. Wagner, J. Ivić, B. Dobrošević, I. Turina, K. Kralik and J. Barbić. 2014. Postoperative immunosuppression markers and the occurrence of sepsis in patients with benign and malignant disease. Wien. Clin. Wochenschr. 126: 774–784.
  • Altun O., M. Almuhayawi, M. Ullberg and V. Ozenci. 2013. Clinical evaluation of the FilmArray Blood Culture Identification Panel in identification of bacteria and yeasts from positive blood culture bottles. J. Clin. Microbiol. 51: 4130–4136.
  • Blaschke A.J., C. Heyrend, C.L. Byington, M.A. Fisher, E. Barker, N.F. Garrone, S.A. Thatcher, A.T. Pavia, T. Barney, G.D. Alger and others. 2012. Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system. Diagn. Microbiol. Infect. Dis. 74: 349–355.
  • Dellinger R.P., M.M. Levy, A. Rhodes, D. Annane, H. Gerlach, S.M. Opal, J.E., Sevransky, C.L. Sprung, I.S. Douglas, R. Jaeschke and others. 2013. Surviving sepsis campaign: international guidelines for management of severe sepsis and septic shock, 2012. Int. Care Med. 39: 165–228.
  • Encina B., L. Lagunes and M. Morales-Codina. 2016. The immunocompromised oncohematological critically ill patient: consideration in severe infections. Ann. Transl. Med. 4: 327–330.
  • Inglis T.J., N. Bzdyl, I.L. Chua, N.M. Urosevic, M.J. Leung and E. Geelhoed. 2016. Improved blood culture identification by Film Array in cultures from regional hospitals compared with teaching hospital cultures. J. Med. Microbiol. 65: 56–61.
  • Kumar A. 2011. Optimizing antimicrobial therapy in sepsis and septic shock. Crit. Care Nurs. Clin. North Am. 23: 79–97.
  • MacVane S.H., J.M. Hurst, M.S. Boger and J.W.Jr. Gnann.2016. Impact of a rapid multiplex polymerase chain reaction blood culture identification technology on outcomes in patients with vancomycin-resistant enterococcal bacteremia. Infect. Dis. (Lond) 48: 732–737.
  • Mahdi H., A. Gojayev, M. Buechel, J. Knight, J. San Marco,D. Lockhart, C. Michener and M. Moslemi-Kebria. 2014. Surgical site infection in women undergoing surgery for gynecologic cancer. Int. J. Gynecol. Cancer. 24: 779–786.
  • Namendys-Silva S.A., J. Texcocano-Becerra, A. Herrera-Gómez. 2010. Prognostic factors in critically ill patients with solid tumours admitted to an oncological intensive care unit. Anaesth. Int. Care 38: 317–324.
  • Otlu B., Y. Bayindir, F. Ozdemir, V. Ince, S. Cuglan, M. Hopoglu, Y. Yakupogullari, C. Kizilkaya, C. Kuzucu, B. Isik and others. 2015. Rapid detection of bloodstream pathogens in liver transplantation patients with FilmArray Multiplex Polymerase Chain Reaction Assays: comparison with conventional methods. Transplant Proc. 47: 1926–1932.
  • Rosolem M.M., L.S. Rabello, T. Lisboa, P. Caruso, R.T. Costa,J.V. Leal, J.I. Salluh and M. Soares. 2012. Critically ill patients with cancer and sepsis: clinical course and prognostic factors. 2012.J. Crit. Care. 27: 301–307.
  • Smit L.C., M.J. Bruins, G.A. Patijn and G.J. Ruijs. 2016. Infectious complications after major abdominal cancer surgery: In search of improvable risk factors. Surg. Infect. (Larchmt.). http:// ncbi.nlm.nih.gov/pubmed/27483132, 2016.08.02 [Epub ahead of print].
  • Zheng X., W. Polanco, D. Carter and S. Shulman. 2014. Rapid identification of pathogens from pediatric blood cultures by use of the FilmArray Blood Culture Identification Panel. J. Clin. Microbiol. 52: 4368–4371.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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