Aspergillus penicillioides Speg. implicated in keratomycosis

• Autorzy: Machowicz-Matejko E. , Furmanczyk A. , Zalewska E.D.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and β-tubulin were carried out for the isolates studied. A high level of similarity was found between sequences from certain rDNA regions, i.e. ITS1-5.8S-ITS2 and LSU, what confirmed the classification of the isolates to the species A. penicillioides. The classification of our isolates to A. penicillioides species was confirmed also by the phylogenetic analysis.

Słowa kluczowe

EN

fungi; Aspergillus penicillioides; morphology; cornea; fungal disease; keratitis; ophthalmic disease; phylogenetic analysis; human disease

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2018
• Tom: 67
• Numer: 4
• Opis fizyczny: p.407-416,fig.,ref.

Twórcy

autor

Machowicz-Matejko E.

• Department of Diagnostics and Microsurgery of Glaucoma, Medical University of Lublin, Lublin, Poland

autor

Furmanczyk A.

• Department of Plant Pathology and Mycology, University of Life Sciences in Lublin, Lublin, Poland

autor

Zalewska E.D.

• Department of Plant Pathology and Mycology, University of Life Sciences in Lublin, Lublin, Poland

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Identyfikatory

DOI

10.21307/pjm-2018-049