Induction of systemic resistance by mixtures of antagonist bacteria for the management of crown rot complex on banana

• Autorzy: Sangeetha G. , Thangavelu R. , Rani S. U. , Muthukumar A. , Udayakumar R.
• Języki publikacji: EN

Abstrakty

EN

Among nine native bacterial strains isolated from banana fruit surface and rhizosphere and six bacterial strains introduced from the culture collection, three native strains viz., non-fluorescent Pseudomonas (NFP₆), Pseudomonas fluorescens (Pf3a), and Bacillus subtilis (BS₁); and two bacterial strains from culture collection viz., Azospirillum (AS1) and Azotobacter (AZ1) have recorded maximum inhibition of mycelial growth of crown rot pathogens (Lasiodiplodia theobromae and Colletotrichum musae) under in vitro condition. When these effective bacterial strains were treated on banana fruits under in vivo, significant reduction of crown rot disease and increased shelf life of banana was observed. However, bacterial strains applied as three way combinations (NFP₆ + Pf3a + BS₁) had greater effect compared with individual and two way combination of bacterial antagonist treatments. The effect of crown rot disease reduction was also comparable to that of fungicide Benomyl (0.1%) both under cold and room temperature storage conditions. Besides, the induction of defense-related enzymes such as phenylalanine ammonia-lyase (PAL), peroxidase (PO), polyphenoloxidase (PPO), and the accumulation of phenolics in banana fruit due to the application of bacterial antagonists were also studied at five different time intervals viz. 0th, 1st, 3rd, 5th and 7th days after treatment. When banana fruits treated with bacterial antagonists (individually and also in different combinations) and challengeinoculated with crown rot pathogens, up to fourfold increase in defense-related enzymes and 3.6 fold increase in phenolic content was observed compared with control. The activity of these defense-related enzymes and phenolic content had gradually increased from 1st day after treatment to 3rd after treatment and reached their peak on 5th day after treatment. Among the bacterial antagonists which have been applied individually and in different combinations, the banana fruits treated with three-way antagonist mixture, i.e., NFP₆ + Pf3a + BS₁ recorded maximum induction of defense-related enzymes and accumulation of phenolics compared with individual and two-way combination of antagonist mixtures. This study suggest that the increased induction of defense-related enzymes and phenolic content due to the treatment of banana fruits with bacterial antagonists might have involved in the reduction of crown rot severity and in turn increased the shelf life of banana fruits.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 6
• Opis fizyczny: p.1177-1187,fig.,ref.

Twórcy

autor

Sangeetha G.

• Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram 608002, Tamil Nadu, India

autor

Thangavelu R.

• National Research Centre for Banana, Thiruchirapalli 620 102, Tamil Nadu, India

autor

Rani S. U.

• Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram 608002, Tamil Nadu, India

autor

Muthukumar A.

• Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram 608002, Tamil Nadu, India

autor

Udayakumar R.

• Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram 608002, Tamil Nadu, India

Bibliografia

• Aharoni Y, Fallik E, Copel A, Gil M, Gringerg S, Klien JD (1997) Sodium bicarbonate reduces post harvest decay development of melons. Postharvest Biol Technol 10:201–206
• Alstrom S (1991) Induction of disease resistance in common bean susceptible to halo blight bacterial pathogen after seed bacterization with rhizosphere pseudomonads. J Gen Appl Microbiol 37:495–501
• Anjaiah V, Koedam N, Nowak-Thompson B, Loper JE, Hofte M, Tambong JT, Cornelis P (1998) Involvement of phenazines and anthranilate in the antagonism of Pseudomonas aeruginosa PNA1 and Tn5 derivative toward Fusarium spp. and Pythium spp. Mol Plant Microbe Interact 11:847–854
• Backman PA, Wilson M, Murphy JF (1997) Bacteria for biological control of plant diseases. In: Rechcigl NA, Rechcigl JE (eds) Environmentally safe approaches to crop disease control. CRC Lewis Publishers, Boca Raton, FL, pp 95–109
• Bell JN, Dixon RA, Bailey JA, Rowell PM, Lamb CJ (1984) Differential induction of chalcone synthase mRNA activity at the onset of phytoalexin accumulation in compatible and in compatible plant–pathogen interactions. Proc Natl Acad Sci USA 81:3384–3388
• Bharathi R, Vivekananthan R, Harish S, Ramanathan A, Samiyappan R (2004) Rhizobacteria-based bioformulations for the management of fruit rot infection in chillies. Crop Prot 23:835–843
• Chalutz E, Wilson CL (1990) Postharvest biocontrol of green and blue mold and sour rot of citrus fruit by Debaryomyces hansenii. Plant Dis 74:134–137
• Chen C, Belanger R, Benhamou N, Paultiz TC (2000) Defense enzymes induced in cucumber roots by treatment with plant growth promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiol Mol Plant Pathol 56:13–23
• Deborah SD, Palaniswami A, Vidhyasekaran P, Velazhahan R (2001) Time course study of the induction of defense enzymes, phenolics and lignin in rice in response to infection by pathogen and non-pathogen. J Plant Dis Prot 108:204–216
• Dickerson DP, Pascholati SF, Hagerman AE, Butler LG, Nicholson RL (1984) Phenylalanine ammonia-lyase and hydroxy cinnamate: CoA ligase in maize mesocotyls inoculated with Helminthosporium maydis or Helminthosporium carbonum. Physiol Plant Pathol 25:111–123
• Duffy BK, Weller DM (1995) Use of Gaumannomyces graminis var graminis alone and in combination with fluorescent Pseudomonas spp. to suppress take all of wheat. Plant Dis 79:907–911
• Finlay AR, Brown AE (1993) The relative importance of Colletotrichum musae as a crown-rot pathogen on Windward Island bananas. Plant Pathol 42:67–74
• Fukui R, Schroth MN, Hendson M, Hancock JG (1994) Interaction between strains of Pseudomonads in sugar beet sphermospheres and the relationship to pericarp colonization by Pythium ultimum in soil. Phytopathology 84:1322–1330
• Gomez KA, Gomez AA (1984) Statistical procedure for agricultural research. Wiley, New York, p 680
• Gross GG (1980) The biochemistry of lignification. Adv Bot Res 8:25–63
• Guetsky R, Shtienberg D, Elad Y, Dinoor A (2001) Combining biocontrol agents to reduce the variability of biological control. Phytopathology 91:621–627
• Hammerschmidt R, Kuc JA (1982) Lignification as a mechanism for induced systemic resistance in cucumber. Physiol Plant Pathol 20:61–71
• Hong CX, Michailides TJ, Holtz BA (1998) Effects of wounding, inoculum density, and biological control agents on postharvest brown rot of stone fruits. Plant Dis 82:210–1216
• Ingham JL (1972) Phytoalexins and other natural products as factors in plant disease. Bot Rev 38:343–424
• Janisiewicz WJ, Korsten L (2002) Biological control of post harvest diseases of fruits. Annu Rev Phytopathol 40:411–441
• Janisiewicz W, Yourman L, Roitman J, Mahoney N (1991) Post harvest control of blue mold and gray mold of apples and pears by dip treatment with pyrrolnitrin, a metabolite of Pseudomonas cepacia. Plant Dis 75:490–494
• Janisiewicz WJ, Peterson DL, Bors R (1994) Control of storage rots of apples with Sporobolomyces roseus. Plant Dis 78:466–470
• Joseph LM, Tan TK, Wong SM (1998) Antifungal effects of hydrogen peroxide and peroxidase on spore germination and mycelial growth of Pseudocercospora species. Can J Bot 76:2119–2124
• Korsten L, De Villiers EE, Wehner FC, Kotze JM (1997) Field sprays of Bacillus subtilis and fungicide for the control of post harvest fruit disease of Avacado in South Africa. Plant Dis 81:455–459
• Krauss U, Mathews P, Bidwell R, Hocart M, Anthony F (2001) Strain discrimination of fungal antagonists of Colletotrichum musae: Implications for biocontrol of crown rot of banana. Mycol Res 105:67–76
• Kuc J, Rush JS (1985) Phytoalexins. Arch Biochem Biophys 236:455–472
• Leeman M, Van Pelt JA, Hendrickx MJ, Scheffer RJ, Bakker PAHM, Schippers B (1995) Biocontrol of Fusarium wilt of radish in commercial greenhouse trials by seed treatment with Pseudomonas fluorescens WCS374. Phytopathology 85:1301–1305
• Leeman M, Den Ouden FM, Van Pelt J, Cornelissen C, Matamala-Garros A, Bakker PAHM, Schippers B (1996) Suppression of Fusarium wilt of radish by co-inoculation of fluorescent Pseudomonas spp. and root colonizing fungi. Eur J Plant Pathol 102:21–31
• Leibinger W, Beuker B, Hahn M, Mendgen K (1997) Control of post harvest pathogens and colonization of the apple surface by antagonistic microorganisms in the field. Phytopathology 87:1103–1110
• Liu L, Kloepper JW, Tuzun S (1995a) Induction of systemic resistance in cucumber against bacterial angular leaf spot by plant growth-promoting rhizobacteria. Phytopathology 85:843–847
• Liu L, Kloepper JW, Tuzun S (1995b) Induction of systemic resistance in cucumber by plant growth-promoting rhizobacteria: duration of protection and effect of host resistance on protection and root colonization. Phytopathology 85:1064–1068
• M’Piga P, Belanger RR, Paulitz TC, Benhamou N (1997) Increased resistance to Fusarium oxysporum f. sp. radicis lycopersici in tomato plants treated with endophytic bacterium Pseudomonas fluorescens strain 63-28. Physiol Mol Plant Pathol 50:01–320
• Mari M, Guizzardi M, Pratella GC (1996a) Biological control of grey mold in pears by antagonistic bacteria. Biol Control 7:30–37
• Mari M, Guizzardi M, Brunelli M, Folchi A (1996b) Postharvest biologicalcontrol of grey mould (Botrytis cinerea Pers.: Fr.) on fresh-market tomatoes with Bacillus amyloliquefaciens. Crop Prot 15:99–705
• Maurhofer M, Hase C, Meuwly P, Metraux JP, Defago G (1994) Induction of systemic resistance of tobacco to tobacco necrosis virus by the root-colonizing Pseudomonas fluorescens strain CHAO: influence of the gacA gene and of pyoverdine production. Phytopathology 84:139–149
• Mayer AM, Harel E, Shaul RB (1965) Assay of catechol oxidase: a critical comparison of methods. Phytochem 5:783–789
• Meena B, Radhajeyalakshmi R, Marimuthu T, Vidhyasekaran P, Sabitha D, Velazhahan R (1999) Induction of pathogenesis-related proteins, phenolics and phenylalanine ammonia-lyase in groundnut by Pseudomonas fluorescens. J Plant Dis Prot 107:4–527
• Morpurgo R, Lopato SV, Afza R, Novak FJ (1994) Selection parameters for resistance to Fusarium oxysporum f. sp. cubense race 1 and race 4 on diploid banana (Musa acuminata Colla). Euphytica 75:121–129
• Persely (ed) (1993) Diseases of fruit crops. Queensland Government, Department of primary industries, Brisbane, Australia
• Pierson EA, Weller DM (1994) Use of mixtures of fluorescent pseudomonads to suppress take all and improve growth in wheat. Phytopathology 84:940–947
• Ploetz RC, Zentmyer GA, Nisijima WT (1994) Compendium of tropical fruit disease. American Phytopathological Society Press, Oregon, p 134
• Pusey PL, Wilson CH (1984) Postharvest biological control of stone fruits brown rot by Bacillus subtilis. Plant Dis 72:753–756
• Ramamoorthy V, Raguchander T, Samiyappan R (2002) Induction of defense-related proteins in tomato roots treated with Pseudomonas fluorescens Pf1 and Fusarium oxysporum f. sp. lycoperisci. Plant Soil 239:55–68
• Reimers PJ, Guo A, Leach JE (1992) In creased activity of cationic peroxidase associated with an in compatible inter action between Xanthomonas oryzae pv oryzae and rice (Oryza sativa). Plant Physiol 99:44–1050
• Sariah M, Lim CL, Tariq SA (2001) Use of biochemical markers as a measure of resistance towards Fusarium wilt of banana. In: Molina AB, Nik Masdek NH, Liew KW (eds) Banana Fusarium wilt management: towards sustainable cultivation. INIBAP Asia and Pacific Network, Los Baňos, pp 243–249
• Schaad NW (1992) Laboratory guide for identification of plant pathogenic bacteria. In: Schaad NW (ed) The American Phytopathological Society. International Book distributing Co., Lucknow, India, pp 60–80
• Smilanick JL (1994) Strategies for the isolation and testing of biocontrol agents. In: Wilson CL, Wisniewski ME (eds) Biological control of postharvest diseases. Theory and practice. CRC Press, Boca Raton, FL, USA, pp 25–41
• Smilanick JL, Dennis-Arrue R (1992) Control of green mold of lemons with Pseudomonas species. Plant Dis 76:481–485
• Thompson DC (1996) Evaluation of bacterial antagonists for reduction of summer patch symptoms in Kentucky blue grass. Plant Dis 80:856–862
• Van Peer R, Niemann GJ, Schippers B (1991) Induced resistance and phytoalexin accumulation in biological control of Fusarium wilt of carnation by Pseudomonas sp. strain WCS417r. Phytopathology 81:728–734
• Velazhahan R, Vidhyasekaran P (1994) Role of pheno lic com pounds, peroxidase and polyphenol-oxidase in resistance of ground nut to rust. Acta Phytopathol Entomol Hung 29:23–29
• Viswanathan R, Samiyappan R (1999) Induction of systemic resistance by plant growth promoting rhizobacteria against red rot disease in sugarcane. Sugar Tech 1:67–76
• Ward ER, Uknes SJ, Williams SC, Dincher SS, Widerhold DL, Alexander DC, Ahl-Goy P, Mefraux JP, Ryals JA (1991) Coordinate gene activity in response to agents that induce systemic acquired resistance. Plant Cell 3:1085–1094
• Wei G, Kloepper JW, Tuzun S (1991) Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria. Phytopathology 81:1508–1512
• Wilson CL, Wisniewski M (1989) Biological control of post harvest diseases of fruits and vegetables: an emerging technology. Annu Rev Phytopathol 27:425–441
• Wilson CL, Wisniewski ME, Biles CL, McLaughlin R, Chalutz E, Droby E (1991) Biological control of post-harvest diseases of fruits and vegetables: alternatives to synthetic fungicides. Crop Prot 10:172–177
• Wisniewski ME, Wilson CL (1992) Biological control of postharvest diseases of fruits and vegetables: recent advances. HortScience 27:94–98
• Wisniewski M, Wilson C, El-Ghaouth A, Droby S (2001) Nonchemical approaches to postharvest disease control. Acta Hortic 553:407–412
• Xue L, Charest PM, Jabaji-Hare SH (1998) Systemic induction of Peroxidases, ß, 1–3, glucanases, chitinases and resistance in bean plants by binucleate Rhizoctonia species. Phytopathology 88:359–365
• Yedidia I, Benhamou N, Chet I (1999) Induction of defense responses in cucumber plants (Cucumis sativus L.) by the bio control agent Trichoderma harzianum. Appl Environ Microbiol 65:1061–1070
• Zahavi T, Cohen L, Weiss B, Schena L, Daus A, Kaplunov T, Zutkhi J, Ben-Arie R, Droby S (2000) Biological control of Botrytis, Aspergillus and Rhizopus rots on table and wine grapes in Israel. Postharvest Biol Technol 20:115–124
• Zdor RE, Anderson AJ (1992) Influence of root colonizing bacteria on the defense responses of bean. Plant Soil 140:99–107
• Zieslin N, Ben-Zaken R (1993) Peroxidase activity and presence of phenolic substances in peduncles of rose flowers. Plant Physiol Biochem 31:333–339