PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 31 | 1 |

Tytuł artykułu

Expression of defence-related peroxidases Prx7 and Prx8 during abiotic stresses in barley roots

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The expression of defence-related peroxidases Prx7 and Prx8 in barley roots grown under selected abiotic stress conditions (toxic metals: Cd, Al, Co, Cu, Hg; drought, salinity, extreme temperatures: heat, cold) and compounds activating (2,4-D) or inhibiting (SHAM) POD activity as well as H₂O₂ and H₂O₂ scavenger (DTT) was characterized. Strong Cd concentration dependent expression of Prx8 peroxidase gene was observed, which correlated with root growth inhibition induced by Cd-and some other stress factors (heavy metals, heat and salinity). Application of H₂O₂ did not cause changes in expression of Prx8, but H₂O₂ scavenger (DTT) as well as the inhibitor (SHAM) and the activator (2,4-D) of PODs induced increase in Prx8 expression. Our results demonstrate that root growth inhibition during any disturbance of active oxygen species (AOS) in root tissue is correlated with upregulation of Prx8 gene expression in barley roots.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

31

Numer

1

Opis fizyczny

p.139-144,fig.,ref.

Twórcy

autor
  • Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 14, 845 23 Bratislava, Slovakia
autor
  • Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 14, 845 23 Bratislava, Slovakia
autor
  • Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 14, 845 23 Bratislava, Slovakia
autor
  • Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 14, 845 23 Bratislava, Slovakia

Bibliografia

  • Bassam BJ, Caetano-Anolles G (1993) Silver staining of DNA in polyacrylamide gels. Appl Biochem Biotechnol 42:181–188
  • Brouwer KS, van Valen T, Day DA, Lambers H (1986) Hydroxamatestimulated O₂ uptake in roots of Pisum sativum and Zea mays, mediated by a peroxidase. Plant Physiol 82:236–240
  • Burbridge E, Diamond M, Dix PJ, McCabe PF (2006) Use of cell morphology to evaluate the effect of a peroxidase gene on cell death induction thresholds in tobacco. Plant Sci 171:139–146
  • Chen G-X, Asada K (1992) Inactivation of ascorbate peroxidase by thiols requires hydrogen peroxide. Plant Cell Physiol 33:117–123
  • Fan L, Linker R, Gepstein S, Tanimoto E, Yamamoto R, Neumann PM (2006) Progressive inhibition by water deficit of cell wall extensibility and growth along the elongation zone of maize roots is related to increased lignin metabolism and progressive stellar accumulation of wall phenolics. Plant Physiol 140: 603–612
  • González LF, Rojas MC (1999) Role of wall peroxidases in oat growth inhibition by DIMBOA. Phytochemistry 50:931–937
  • Hiraga S, Yamamoto K, Ito H, Sasaki K, Matsui H, Honma M, Nagamura Y, Sasaki T, Ohashi Y (2000) Diverse expression profiles of 21 rice peroxidase genes. FEBS Lett 471:245–250
  • Hiraga S, Sasaki K, Ito H, Ohashi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol 42:462–468
  • Katsuhara M, Otsuka T, Ezaki B (2005) Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis. Plant Sci 169:369–373
  • Kim YH, Kim CY, Song WY, Park DS, Kwon SY, Lee HS, Bang JW, Kwak SS (2008) Overexpression of sweetpotato swpa4 peroxidase results in increased hydrogen peroxide production and enhances stress tolerance in tobacco. Planta 227:867–881
  • Kristensen BK, Brandt J, Bojsen K, Thordal-Christensen H, Kerby KB, Collinge DB, Mikkelsen JD, Rasmussen SK (1997) Expression of a defence-related intercellular barley peroxidase in transgenic tobacco. Plant Sci 122:173–182
  • Kristensen BK, Bloch H, Rasmussen SK (1999) Barley coleoptile peroxidases. Purification, molecular cloning, and induction by pathogens. Plant Physiol 120:501–512
  • Lin CC, Kao CH (1999) NaCl induced changes in ionically bound peroxidase activity in roots of rice seedlings. Plant Soil 216: 147–153
  • Lin CC, Kao CH (2002) Osmotic stress-induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Growth Regul 37:177–184
  • Liszkay A, van der Zalm E, Schopfer P (2004) Production of reactive oxygen intermediates (O₂˙⁻, H₂O₂, and˙OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol 136:3114–3123
  • Liu G, Sheng X, Greenshields DL, Ogioglo A, Kaminskyj S, Selvaraj G, Wei Y (2005) Profiling of wheat class III peroxidase genes derived from powdery mildew-attacked epidermis reveals distinct sequence-associated expression patterns. Mol Plant Microbe Interact 18:730–741
  • MacAdam JW, Sharp RE, Nelson CJ (1992) Peroxidase activity in the leaf elongation zone of tall fescue. Plant Physiol 99:879–885
  • Mika A, Minibayeva F, Beckett R, Lüthje S (2004) Possible function of extracellular peroxidases in stress-induced generation and detoxification of active oxygen species. Phytochem Rev 3:173–193
  • Pichorner H, Couperus A, Korori SAA, Ebermann R (1992) Plant peroxidases has thiol oxidase function. Phytochemistry 31: 3371–3376
  • Ryan PR, Ditomaso JM, Kochian LV (1993) Aluminium toxicity in roots—an investigation of spatial sensitivity and the role of the root cap. J Exp Biol 44:437–446
  • Sasaki K, Iwai T, Hiraga S, Kuroda K, Seo S, Mitsuhara I, Miyasaka A, Iwano M, Ito H, Matsui H, Ohashi Y (2004) Ten rice peroxideses redundantly respond to multiple stresses including infection with rice blast fungus. Plant Cell Physiol 45:1442–1452
  • Sävenstrand H, Strid Å (2004) Six genes strongly regulated by mercury in Pisum sativum roots. Plant Physiol Biochem 42: 135–142
  • Scott-Craig JS, Kerby KB, Stein BD, Somerville SC (1995) Expression of an extracellular peroxidase that is induced in barley (Hordeum vulgare) by powdery mildew pathogen (Erysiphe graminis f. sp. hordei). Physiol Mol Plant Pathol 47:407–418
  • Schopfer P (1996) Hydrogen peroxide mediated cell wall stiffening in vitro. Planta 199:43–49
  • Schützendübel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A (2001) Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots. Plant Physiol 127:887–898
  • Siegel BZ (1993) Plant peroxidases—an organismic perspective. Plant Growth Regul 12:303–312
  • Šimonovičová M, Huttová J, Mistrík I, Široká B, Tamás L (2004a) Peroxidase mediated hydrogen peroxide production in barley roots grown under stress conditions. Plant Growth Regul 44:267–275
  • Šimonovičová M, Huttová J, Mistrík I, Široká B, Tamás L (2004b) Root growth inhibition by aluminum is probably caused by cell death due to peroxidase-mediated hydrogen peroxide production. Protoplasma 224:91–98
  • Stoessl A (1967) The antifungal factors in Barley IV. Isolation, structure, and synthesis of the hordatines. Can J Chem 45:1745–1760
  • Šukalović VH, Vuletić M, Vučinić Ž (2005) The role of p-coumaric acid in oxidative and peroxidative cycle of the ionically bound peroxidase of the maize root cell wall. Plant Sci 168:931–938
  • Suzuki N, Mittler R (2006) Reactive oxygen species and temperature stresses: a delicate balance between signaling and destruction. Physiol Plant 126:45–51
  • Tamás L, Huttová J, Mistrík I (2003) Inhibition of Al-induced root elongation and enhancement of Al-induced peroxidase in Alsensitive and Al-resistant barley cultivars are positively correlated. Plant Soil 250:193–200
  • Tamás L, Huttová J, Mistrík I, Ollé M (2006) Cadmium-induced microsomal membrane-bound peroxidases mediated hydrogen peroxide production in barley roots. Acta Physiol Plant 28:453–457
  • Thordal-Christensen H, Brandt J, Cho BH, Rassmussen SK, Gregersen PL, Smedegaard-Petersen V, Collinge DB (1992) cDNA cloning and characterization of two barley peroxidase transcripts induced differentially by the powdery mildew fungus Erysiphe graminis. Physiol Mol Plant Pathol 40:395–409
  • Vale GP, Torrigiani E, Gatti A, Delogu G, Porta-Puglia A, Vannacci G, Cattivelli L (1994) Activation of genes in barley roots in response to infection by two Drechslera graminea isolates. Physiol Mol Plant Pathol 44:207–215
  • Welinder KG, Justesen AF, Kjærsgård IVH, Jensen RB, Rasmussen SK, Jespersen HM, Duroux L (2002) Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana. Eur J Biochem 269:6063–6081

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-dee5606d-8280-4b17-bb0c-515a69a5b036
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.