Intraperitoneal delivery of ex vivo activated macrophages results in depression-like behavior of mice

• Autorzy: Roman A. , Kusmierczyk J. , Klimek E. , Nalepa I.
• Języki publikacji: EN

Abstrakty

EN

Excessive pro-inflammatory activity of macrophages is regarded as one of pathomechanisms of depressive disorders. Administration of lipopolysaccharide (LPS) induces potent activation of whole immune system and is used as an animal model of depression. In this study we investigated whether macrophages, previously activated ex vivo and delivered to mice, are able to induce the behavioral changes related to depression. The experiment was carried out on male C57BL/6J mice. Peritoneal macrophages were stimulated in vitro with LPS for 3 h. Then they were stained with 5(6)- carboxyfluorescein diacetate N-succinimidyl ester and injected intraperitoneally at the dose of 2×106 cells per mouse. Control group was administered likewise with non-stimulated cells. Twenty hours later the recipients were subjected to tests of depressive-like behavior including the locomotor activity, the tail suspension test and the forced swimming test (FST). Presence of injected cells in various compartments of the body was assessed 28 h after administration using flow cytometry and fluorescence microscopy. We found that the administration of ex vivo activated macrophages caused a decrease of initial exploratory activity of the mice (by 26%) and decreased struggling behavior in the FST (by 65%) in comparison to animals administered with non-stimulated cells. Majority of administered macrophages went away from the peritoneum. They were absent in spleen, in lymph nodes and in pleural cavity but were present in blood. Activated macrophages were more mobile than non-stimulated cells (below 1% and about 5% of peritoneal cells, respectively). These results suggest that ex vivo activated macrophages are able to change some parameters of recipients’ behavior in depressive-like fashion. The fate of activated cells varies as they are more mobile in the recipients’ body as compared with non-activated cells. Supported by a grant POIG.01.01.02-12-004/09-00 financed by European Regional Development Fund.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.63-64

Twórcy

autor

Roman A.

• Department of Brain Biochemistry, Institute of Pharmacology PAS, Krakow, Poland

autor

Kusmierczyk J.

• Department of Brain Biochemistry, Institute of Pharmacology PAS, Krakow, Poland

autor

Klimek E.

• Department of Brain Biochemistry, Institute of Pharmacology PAS, Krakow, Poland

autor

Nalepa I.

• Department of Brain Biochemistry, Institute of Pharmacology PAS, Krakow, Poland

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