TRP channels participare in memory formation in passive avoidance task in one day old chicks

• Autorzy: Salinska E. , Stafiej A. , Slomka M.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Influx of calcium ions (Ca2+) into neurons after stimulation of glutamate receptors is a crucial step in intracellular cascade of memory formation. Recent findings showed the existence of additional mechanism involved in intracellular Ca2+ increase and triggered not by external signal but by internal signals like increase of Ca2+ within the cell and activation of G protein coupled receptors. We are talking here about transient receptor potential (TRP) channels. The aim of our study was to investigate the participation of TRP channels, especially TRPC and TRPV in intracellular mechanisms engaged in memory consolidation. METHODS: The model of passive avoidance task on one day old chicks was used. Chicks were injected with TRP channels antagonist SKF96365 and three different concentrations of 2-APB, the inhibitor of IP3 receptors, which in small concentrations inhibits also TRP channels. The injections were made at different times before and after training, to find the most effective time of interference. RESULTS: We found that injection of all antagonists immediately after training resulted in task amnesia when tested 24 h later. The amnesic effect of injection of SKF96365 or 2-APB immediately after training was tested at different times. It appeared that SKF96365 injection resulted in constant amnesia that manifested 1.5 h after training, whereas amnesia after injection of 2-APB was observed as early as 30 min after training. The effect of application of TRP channels antagonist SKF96365 on memorizing of the task in comparison with the effects of mGluR1 and mGlR5 antagonists showed similarities when memory was tested 2 h and 24 h training. CONCLUSIONS: Our results show that inhibition of TRP channels results in disturbance in memory formation and that inhibition of both TRP channels and IP3 receptors using small concentrations of 2-APB has a strong impact on this process.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S40-S41

Twórcy

autor

Salinska E.

• Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Stafiej A.

• Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Slomka M.

• Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland