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2012 | 62 | 1 |

Tytuł artykułu

Phenolic compounds and seed oil composition of Ziziphus mauritiana L. fruit

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Ber is a tropical fruit which grows from the tree species, Ziziphus mauritiana Lamk. The pericarp of this fruit is consumed either fresh or dried while its seeds are usually discarded as waste. The present study was undertaken to evaluate the antioxidant activity and phenolic content of the fruit, and to evaluate if any potential value-added phytochemicals can be extracted from seed waste. The edible portion of the fruit was extracted with 60% aqueous methanol by sonication and then assayed for total phenolic content, antioxidant activity, and individual phenolic compounds by HPLC-DAD. The seed oil extracted with n-hexane was assayed for fatty acid composition, sterols, and tocopherols content by GC-MS. The total phenolic content of the fresh fruit was 12.8 mg/g as gallic acid equivalent, with an antioxidant activity of 0.5 μmol/g as quercetin equivalent by Folin-Ciocalteu and DPPH assays respectively. Hydroxybenzoic acid, vanillin, ortho- and para-coumaric acid, epicatechin, quercetin, and naringenin were tentatively identifi ed by matching retention time and UV spectra with those of commercial reference standards. GC-MS analysis of the TMS derivative of fruit extract showed the presence of following compounds: propanoic, hexanoic, heptanoic, octanoic, nonanoic, decanoic, dodecanoic, n-pentadecanoic, hexadecanoic, benzoic, and trihydroxybenzoic acids. In addition, D-fructose, galactofuranoside, gluconic acid, and β-sitosterol were also detected. In seed oil of ber, the fatty acids such as, hexanoic, octanoic, 7-octadecenoic, 9,12-octadecendoic, eicosanoic, 11-eicosenoic, and docosanoic acid with 7-octadecenoic acid, were found to make up 55% of total fatty acids. Squalene, γ-tocopherol and stigmasterol were identifi ed as minor constituents in the unsaponifi able fraction of seed oil. Current study shows that ber fruit is a good source of healthy phytochemicals.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

62

Numer

1

Opis fizyczny

p.15-21,fig.,ref.

Twórcy

autor
  • National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan
autor
autor

Bibliografia

  • 1. Anonymous, Fruit, vegetables and condiments statistics of Pakistan (Report). Ministry of Food, Agriculture and Livestock, Islamabad, Pakistan, 2006–07, http://www.minfal.gov.pk/.
  • 2. Arain S., Sherazi S.T.H., Bhanger M.I., Mahesar S.A., Memon N., Physiochemical characterization of Bauhinia purpurea seed oil and meal for nutritional exploration. Pol. J. Food Nutr. Sci., 2010, 60, 341–346.
  • 3. Azam-Ali S., Bonkoungou E., Bowe C., DeKok C., Godara A., Williams J. T., Ber and other jujubes. 2006, in: Fruits for the Future (eds. J.T.Williams, R.W.Smith, N. Haq, Z.Dunsigaer). Southampton Centre for Underutilized Crops, Southhampton,
  • p. 1, 19 and 29. 4. Balasundram N., Sundram K., Samman S., Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem., 2006, 99, 191–203.
  • 5. Cert A., Moreda W., Pérez-Camino M.C., Chromatographic analysis of minor constituents in vegetable oils. J. Chromatogr. A, 2000, 881, 131–148.
  • 6. Guo S., Duan J.-A., Tang Y.-P., Yang N.-Y., Qian D.-W., Su S.- L., Shang E.-X., Characterization of triterpenic acids in fruits of Ziziphus species by HPLC-ELSD-MS. J. Agric. Food. Chem., 2010, 58, 6285–6289.
  • 7. Iqbal S., Bhanger M.I., Anwar F., Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chem., 2005, 93, 265–272.
  • 8. Kredy H.M., Huang D., Xie B., He H., Yang E., Tian B., Xiao D., Flavonols of lotus (Nelumbo nucifera, Gaertn.) seed epicarp and their antioxidant potential. Eur. Food Res. Technol., 2010, 231, 387–394.
  • 9. Lin L.-Z., Harnly J.M., A screening method for the identifi cation of glycosylated fl avonoids and other phenolic compounds using a standard analytical approach for all plant materials. J. Agric. Food. Chem., 2007, 55, 1084–1096.
  • 10. Luthria D.L., Signifi cance of sample preparation in developing analytical methodologies for accurate estimation of bioactive compounds in functional foods. J. Sci. Food Agric., 2006a, 86, 2266–2272.
  • 11. Luthria D.L., Application of green chemistry principles for extraction of phytolipids and phenolic compounds. Indian J. Chem. Sect. B., 2006b, 45, 2291–2296.
  • 12. Memon A.A., Memon N., Luthria D.L., Bhanger M.I., Pitafi A.A., Phenolic acids profi ling and antioxidant potential of mulberry (Morus laevigata W., Morus nigra L., Morus alba L.) leaves and fruits grown in Pakistan. Pol. J. Food Nutr. Sci., 2010, 60, 25–32.
  • 13. Morton J.M., Indian Jujube. 1987, in: Fruits of Warm Climates. J.M. Morton, Miami, FL, pp. 272–275.
  • 14. Muchuweti M., Zenda G., Ndhlala A. R., Kasiyamhuru A., Sugars, organic acid and phenolic compounds of Ziziphus mauritiana fruit. Eur. Food Res. Technol., 2005, 221, 570–574.
  • 15. Obeed R.S., Harhash M.M., Abdel-Mawgood A.L., Fruit properties and genetic diversity of fi ve ber (Ziziphus mauritiana lamk) cultivars. Pak. J. Biol. Sci., 2008, 11, 888–893.
  • 16. Paquot C., Standard methods for the analysis of oils, fats and derivatives. International Union of Pure and Applied Chemistry, 1992,
  • 17. Phillips K.M., Ruggio D.M., Toivo J.I., Swank M.A., Simpkins A.H., Free and esterifi ed sterol composition of edible oils and fats. J. Food Comp. Anal., 2002, 15, 123–142.
  • 18. Ramadan M.F., Morsel J.-T., Oil goldenberry (Physalis peruviana L.). J. Agri. Food Chem., 2003, 51, 969–974.
  • 19. Reichert R.D., Oilseed medicinals: In natural drugs, dietary supplements and in new functional foods. Trends Food Sci. Technol., 2002, 13, 353–360.
  • 20. de Souza R.F.V., de Giovani W.F., Antioxidant properties of complexes of fl avonoids with metal ions. Redox Report, 2004, 9, 97–104.
  • 21. Sepúlveda E., Sáenz C., Aliaga E., Aceituno C., Extraction and characterization of mucilage in Opuntia spp. J. Arid Environ., 2007, 68, 534–545.
  • 22. Stevenson D.G., Eller F.J., Wang L., Jane J.-L., Wang T., Inglett G.E., Oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. J. Agric. Food. Chem., 2007, 55, 4005–4013.

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Bibliografia

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