Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia

• Autorzy: Chaabani G. , Tabart J. , Kevers C. , Dommes J. , Khan M.I. , Zaoui S. , Chebchoub L. , Lachaal M. , Karray-Bouraoui N.
• Języki publikacji: EN

Abstrakty

EN

The present research work describes the effects of 2,4-dichlorophenoxyacetic acid (2,4-D)/Benzylaminopurine (BAP) ratio on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf-derived calli of Crataegus azarolus (hawthorn). The supplementation of 1.0 mg/L 2,4-D and 1.0 mg/L BAP to MS medium was found to be the most efficient for callus induction (as percentage and fresh weigh). The results of biochemical analysis showed that the highest total phenolic contents were obtained in callus cultured on MS medium supplemented with 2.0 mg/L 2,4- D and 1.0 mg/L BAP (52 – 0.56 mg GAE/g DM) and were significantly lower than those of intact leaves (76 – 1.72 mg GAE/g DM). However, the highest ascorbic acid contents were found in callus cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 0.5 mg/L BAP (0.96 – 0.13 mg AAE/g DM) and these amounts were statistically similar to those found in leaf tissues (0.74 – 0.07 mg AAE/g DM). Antioxidant activities of callus extracts were determined using two TEAC assays and results showed that extract of callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP have the greatest antiradical activities against DPPH (124 – 2.92 mg TE/g DM) and ABTS (0.19 – 0.02 mg TE/g DM) compared to the leaves of field-grown plant. Thus, the use of high level of 2,4-D over BAP can be suitable to enhance the quality more than the quantity of bioactive compounds in leaf callus culture of hawthorn.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 02
• Opis fizyczny: Article: 16 [9 p.], fig.,ref.

Twórcy

autor

Chaabani G.

• Physiology and Biochemistry of Plant Response to Abiotic Stresses Unit, Faculty of Sciences of Tunis, University of Tunis El Manar, 1060 Tunis El Manar, Tunisia

autor

Tabart J.

• Plant Molecular Biology and Biotechnology Unit, CEDEVIT (ASBL), B22, Plant Biology Institute, University of Liege, San Tilman, 4000 Liege, Belgium

autor

Kevers C.

• Plant Molecular Biology and Biotechnology Unit, CEDEVIT (ASBL), B22, Plant Biology Institute, University of Liege, San Tilman, 4000 Liege, Belgium

autor

Dommes J.

• Plant Molecular Biology and Biotechnology Unit, CEDEVIT (ASBL), B22, Plant Biology Institute, University of Liege, San Tilman, 4000 Liege, Belgium

autor

Khan M.I.

• Department of Weed Science, University of Agriculture, Peshawar 25130, Pakistan

autor

Zaoui S.

• Physiology and Biochemistry of Plant Response to Abiotic Stresses Unit, Faculty of Sciences of Tunis, University of Tunis El Manar, 1060 Tunis El Manar, Tunisia

autor

Chebchoub L.

• Laboratory of In Vitro Culture, Support Station of Manouba, Professional Association of Vegetables, 2010 Manouba, Tunisia

autor

Lachaal M.

• Physiology and Biochemistry of Plant Response to Abiotic Stresses Unit, Faculty of Sciences of Tunis, University of Tunis El Manar, 1060 Tunis El Manar, Tunisia

autor

Karray-Bouraoui N.

• Physiology and Biochemistry of Plant Response to Abiotic Stresses Unit, Faculty of Sciences of Tunis, University of Tunis El Manar, 1060 Tunis El Manar, Tunisia

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Identyfikatory

DOI

10.1007/s11738-014-1769-4