Expression of three diadinoxanthin de-epoxidase genes of Phaeodacylum tricornutum in Escherichia coli Origami b and BL21 strain

• Autorzy: Bojko M. , Olchawa-Pajor M. , Tuleja U. , Kuczynska P. , Strzalka W. , Latowski D. , Strzalka K.
• Języki publikacji: EN

Abstrakty

EN

In the diadinoxanthin cycle the epoxy group is removed from diadinoxanthin and diatoxanthin is created. This conversion takes place e.g. in diatoms with the involvement of the enzyme diadinoxanthin de-epoxidase. In one of the diatom species, Phaeodactylum tricornutum (CCAP 1055/1 strain with genome sequenced) three de-epoxidase genes (PtVDE, PtVDL1, PtVDL2) have been identified, but only one of them (PtVDE) corresponds to violaxanthin de-epoxidase, an enzyme which is commonly found in higher plants. In these studies, the expression of two de-epoxidase genes of another Phaeodactylum tricornutum strain (UTEX 646), which is commonly used in diatom studies, were obtained in Origami b and BL21 E. coli strains. The molecular masses of the mature proteins are about 49 kDa and 60 kDa, respectively, for VDE and VDL2. Both enzymes are active with violaxanthin as a substrate.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 4
• Opis fizyczny: p.857-860,fig.,ref.

Twórcy

autor

Bojko M.

• Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland

autor

Olchawa-Pajor M.

• Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland

autor

Tuleja U.

• Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland

autor

Kuczynska P.

• Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland

autor

Strzalka W.

• Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland

autor

Latowski D.

• Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland

autor

Strzalka K.

• Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland

Bibliografia

• Coesel S, Obornik M, Varela J, Falciatore A, Bowler Ch (2008) Evolutionary Origins and function of the carotenoid biosynthetic pathway in marine diatoms. PLoS ONE 3: e2896 1-16. 
• Dugdale RC, Wilkerson FP (1998) Silicate regulation of new production in the equatorial Pacific upwelling. Nature 391: 270-273.
• Egge JK, Aksnes DL (1992) Silicate as regulating nutrient in phytoplankton competition. Mar Ecol Prog Ser 83: 281-289.
• Geider RJ, Delucia EH, Falkowski PG, et al. (2001) Primary productivity of planet earth: biological determinants and physical constraints in terrestrial and aquatic habitats. Global Change Biol 7: 849-882.
• Goss R, Jakob T (2010) Regulation and function of xantophyll cycle-dependent photoprotection in algae. Photosynth Res 106: 103-122.  
• Guillard RR, Ryther JH (1962) Studies of marine planktonic diatoms: I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can J Microbiol 8: 229-239. 
• Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In Culture of Marine Invertebrate Animals, Smith WL, Chanley MH eds, pp 26-60. Plenum Press, New York, USA. 
• Jahns P, Latowski D, Strzałka K (2009) Mechanism and regulation of violaxanthin cycle: The role of antenna proteins and membrane lipids. Biochim Biophys Acta 1787: 3-14. 
• Latowski D, Kruk J, Burda K, Skrzynecka-Jaskier M, Kostecka-Gugała A, Strzałka K (2002) Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxydase, a xantophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers. Eur J Biochem 269: 4656-4665. 
• Siaut M, Heijde M, Mangogna M, Montsant A, Coesel S, Allen A, Manfredonia A, Falciatore A, Bowler C (2007) Molecular toolbox for studying diatom biology in Pheodactylum tricornutum. Gene 406: 23-35. 
• Smetacek VS (1985) Role of sinking in diatom life-history cycles: Ecological, evolutionary and geological significance. Mar Biol 84: 239-251.
• Swords WS (2003) Chemical transformation of E. coli. In Methods in Molecular Biology. E. coli plasmid vectors. Casali N, Preston A eds, vol 234, pp 49-53. Humana Press Inc. Totowa NJ. 
• Treguer P, Nelson DM, Van Bennekom AJ, DeMaster DJ, Leynaert A, Queguiner B (1995) The silica balance in the world ocean: A reestimate. Science 268: 375-379. 
• Yamamoto HY (1985) Xantophyll cycle. Methods Enzymol 110: 303-312.
• Yool A, Tyrrell T (2003) Role of diatoms in regulating the ocean's silicon cycle. Global Biogeochemical Cycles 17: 1103-1124.