Characteristics of Aureobasidium pullulans (de Bary et Lowenthal) G. Arnaud isolated from apples and pears with symptoms of sooty blotch in Poland

• Autorzy: Mirzwa-Mroz E. , Winska-Krysiak M. , Dzieciol R. , Miekus A.

Warianty tytułu

PL

Charakterystyka grzyba Aureobasidium pullulans (de Bary et Lowenthal) G. Arnaud wyizolowanego z jabłek i gruszek z objawami brudnej plamistości w Polsce

• Języki publikacji: EN

Abstrakty

EN

Sooty blotch is a disease of apple and pear caused by a complex of fungi that blemish the fruit surface. Results of molecular studies indicated approximately 30 different fungi species associated with this disease. Apples and pears with symptoms of sooty blotch were collected in summer and early autumn 2006–2010 from trees grown in fungicide non-treated orchards and small gardens located in various regions of Poland. Fungi causing sooty blotch were isolated from fruits and the isolates were divided into six groups, according to their morphological characters. Growth of the fungi colonies were tested on different agar media (PDA, CMA, MEA and Czapek). The ITS region of rDNA from 16 isolates from the first group was amplified by PCR technique and one representative sequence of this isolates was used to alignment in Gene Bank. This isolate was identified as Aureobasidium pullulans and isolates from this group were compared with it on the base of morphological features.

PL

Przyczyną brudnej plamistości jabłek i gruszek jest kompleks grzybów powodujących oszpecenie powierzchni skórki owoców. Wyniki badań molekularnych wskazują, że z tą chorobą wiąże się ponad 30 różnych gatunków grzybów. Jabáka i gruszki z widocznymi objawami brudnej plamistości jabłek zbierano z niechronionych chemicznie sadów i ogródków działkowych w różnych regionach Polski. Owoce byáy zbierane latem i wczesną jesienią w latach 2006–2010. Z plam znajdujących się na owocach wyizolowano sprawców brudnej plamistości jabłek i na podstawie charakterystyki cech morfologicznych uzyskanych izolatów grzybów podzielono je na sześć grup. Badano również wzrost i wygląd kolonii grzybów na różnych podłożach (PDA, CMA, MEA i Czapek). Wyizolowano, a następnie zamplifikowano za pomocą techniki PCR z wykorzystaniem starterów ITS1F i ITS4, genomowe DNA 16 izolatów należących do pierwszej grupy. Grzyb ten został zidentyfikowany jako gatunek Aureobasidium pullulans, a jego zsekwencjonowane izolaty stanowiły wzorzec przy identyfikacji pozostałych izolatów zaklasyfikowanych do tej grupy.

Słowa kluczowe

EN

Aureobasidium pullulans; fungi; isolation; apple; pear; fruit; disease symptom; sooty blotch; Poland; polymerase chain reaction; Gloeodes pomigena

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2014
• Tom: 13
• Numer: 3
• Opis fizyczny: p.13-22,fig.,ref.

Twórcy

autor

Mirzwa-Mroz E.

• Department of Plant Pathology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Winska-Krysiak M.

• Department of Plant Pathology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Dzieciol R.

• Department of Plant Pathology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Miekus A.

• Department of Plant Pathology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

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