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2013 | 16 | 1 |

Tytuł artykułu

Real time PCR detection of rabbit haemorrhagic disease virus in rabbits infected with different European strains of RHDV

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The paper concerns the use of a novel, very effective diagnostic method, a real-time PCR for diagnosis of a viral agent causing viral haemorrhagic disease in rabbits – RHDV. Until now, the method was widely used for detecting many different viruses, both DNA, and RNA, but as far as RHDV is concerned, there are not many records of such use. This study aimed at the detection of 17 different strains from different European regions, differing in biological features and mortality. The study confirmed that real-time PCR is an applicable and effective method for diagnosis of RHDV, irrespective of the stains’ features.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

16

Numer

1

Opis fizyczny

p.39-43,fig.,ref.

Twórcy

  • Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin
  • Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin
autor
  • Department of Microbiology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin
  • Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin
autor
  • Department of Microbiology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin

Bibliografia

  • Bacławski M, Banyłaszewicz U, Brojer E, Grabarczyk P, Mariańska B, Nasiłowska B (2006) Standarization of real-time PCR method for quantitative determination of DNA EBV in blood of patients after allogenic transplantation of bone marrow. Proceedings of the Conference „Biologia molekularna w diagnostyce chorób zakaźnych i biotechnologii”, Warsaw, Poland, pp. 112-116.
  • Bae HG, Nitsche A, Teichmann A, Biel SB, Niedrig M (2003) Detection of yellow fever virus: a comparison of quantitative real- time PCR and plaque assay. J Virol Meth 110: 185-191.
  • Dzieciątkowski T, Łuczak M, Majewska A, Tomaszewska (2007) Infections with human parvovirus B19. Post Microbiol 46: 203-210.
  • Dzieciątkowski T, Przybylski M, Chmielewska A, Tucholska A, Turowska A, Banbura M (2008) Evaluation ot a real-time PCR assay using the LightCycler® system for detection of equine herpesvirus type 1 DNA. Med Weter 64: 918-921.
  • Fitzner A, Niedbalski W, Kęsy A, Paprocka G (2011) Detection of RHD virus by a real-time reverse transcription PCR. Bull Vet Inst Pulawy 55: 581-586.
  • Grabarczyk P, Brojer E, Nasiłowska B (2003) Quantitative analysis of DNA CMV by real-time PCR: standardization of the method and preliminary results in haematological patients. Acta Haematol Pol 34: 447-457.
  • Gurukumar KR, Priyadarshini D, Patil JA, Bhagat A, Singh A, Shah PS, Cecilia D (2009) Development of real-time PCR for detection and quantitation of Dengue viruses. Virol J 6: 10.
  • Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonak J, Lind K, Sindelka R, Sjoback R, Sjogreen B, Strombom L, Stahlberg A, Zoric N (2006) The real-time polymerase chain reaction. Mol Aspects Med 27: 95-125.
  • Kuźmak J, Materiak M, Sieradzki Z (2006) The preparation of nested PCR and real-time Sybr Green PCR tests for detection bovine syncytial virus infections. Proceedings of the Conference „Biologia molekularna w diagnostyce chorób zakaźnych biotechnologii”. Warsaw, Poland, pp. 112-116.
  • Lipiński D, Zeyland J, Słomski R (2008) Real time PCR. In: Słomski R (ed) DNA analysis – the theory and practise. Poznań University of Life Sciences, Poznań, Poland.
  • Niedźwiedzka P (2008) Immunological profile and apoptosis in rabbits experimentally infected with strains of RHD (rabbit haemorrhagic disease) virus with different biological features. Doctoral thesis, Department of Natural Sciences, University of Szczecin, Szczecin, Poland.
  • Niedźwiedzka-Rystwej P, Deptuła W (2010) Non-specific immunity in rabbits infected with 10 strains of the rabbit haemorrhagic disease virus with different biological properties. Centr Europ J Biol 5: 613-632.
  • Niedźwiedzka-Rystwej P, Deptuła W (2012) Lymphocyte subpopulations and apoptosis of immune cells in rabbits experimentally infected with a strain of the RHD virus having a variable haemagglutination capacity. Pol J Vet Sci 15: 43-49.
  • Nowaczyk P (2007) Immune phenomena, apoptosis and viral study with molecular biology methods in rabbits immunized with EBHS (European Brown Hare Syndrome) virus. Doctoral thesis, Department of Natural Sciences, University of Szczecin, Szczecin, Poland.
  • Pejsak Z, Podgórska K, Stadejek KT (2006) Applying real time PCR for detecting pestiviruses. Med Weter 62: 165-169.
  • Radkowski M, Gallegos-Orozco JF, Jablonska J, Colby TV, Walewska-Zielecka B, Kubicka J, Wilkinson J, Adair D, Rakela J, Laskus T (2005) Persistence of hepatitis C virus in patients successfully treated for chronic hepatitis C. Hepatology 41: 106-114.
  • Scaramozzino N, Ferrier-Rembert A, Favier AL, Rothlisberger C, Richard S, Crance JM, Meyer H, Garin D (2007) Real-time PCR to identify variola virus or other human pathogenic orthopox viruses. Clin Chem 53: 606-613.
  • Teixeira L, Marques RM, Aguas AP, Ferreira P (2011) A simple and rapid method for isolation of caliciviruses from liver of infected rabbits. Res Vet Sci 91: 164-166.
  • Tokarz-Deptuła B (2009) Immunity phenomena in rabbits infected with the RHD (rabbit haemorrhagic disease) virus. Pol J Env Stud 7: 1-81.
  • Watzinger F, Ebner K, Lion T (2006) Detection and monitoring of virus infections by real-time PCR. Mol Aspects Med 27: 254-298.

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