Micropropagation of Allium neapolitanum Cirillo

• Autorzy: Stelmaszczuk M. , Kozak D.

Warianty tytułu

PL

Mikrorozmnażanie Allium neapolitanum Cirillo

• Języki publikacji: EN

Abstrakty

EN

Allium neapolitanum is a valuable species of snow-white flowers, which is suitable for cultivation in flowerbeds, rock gardens as well as in containers. Whole buds of Allium neapolitanum were excised from bulbs in the beginning of October and then they were cultured on Murashige and Skoog (MS) medium containing 2 mg BA·dm⁻³ and 0.1 mg NAA·dm⁻³ for shoot initiation. After several passages on the same medium for shoot multiplication, bases of shoots were placed for 2 subcultures on MS medium supplemented with BA or 2-iP in concentration of 2 or 5 mg·dm⁻³ separately or in combination with NAA in concentration of 0.1 or 1 mg·dm⁻³ to obtain multiplication. MS medium without growth regulators was used as a control. The best results were obtained on the medium supplemented with 5 mg BA·dm⁻³ and 0.1 mg NAA·dm⁻³. On average 5.7 shoots regenerated from 1 shoot base during 12 weeks. Three types of auxins, IAA, IBA and NAA in concentration of 0.5 mg·dm⁻³, were used for rooting. It was observed that NAA enhanced root formation but reduced roots length. The best quality rooted shoots were obtained on medium supplemented with 0.5 mg·dm⁻³ IAA. The survival rate of the plantlets under ex vitro condition was 70% after 4 weeks.

PL

Allium neapolitanum to cenny gatunek o ozdobnych śnieżnobiałych kwiatach, nadający się do uprawy na rabatach, skalniakach i w pojemnikach. Do badań wykorzystano całe pąki Allium neapolitanum, które izolowano z cebul na początku października i wykładano na pożywkę Murashige i Skooga (MS) zawierającą 2 mg BA·dm⁻³ i 0,1 mg NAA·dm⁻³ w celu inicjacji pędów. Po kilku pasażach namnażania na tej samej pożywce pobierano podstawę pędów, które kultywowano w ciągu 2 pasaży na pożywce MS uzupełnionej BA lub 2iP w stężeniu 2 lub 5 mg·dm⁻³ pojedynczo lub łącznie z NAA w stężeniu 0,1 lub 1 mg·dm⁻³. Kontrolę stanowiła pożywka MS bez regulatorów wzrostu. Najlepsze wyniki namnażania pędów uzyskano na pożywce z dodatkiem 5 mg BA·dm⁻³ i 0,1 mg NAA·dm⁻³, na której z jednej podstawy pędu otrzymano 5,7 pędów po 12 tygodniach kultury. Do ukorzeniania pędów zastosowano trzy auksyny: IAA, IBA i NAA w stężeniu 0,5 mg·dm⁻³. W obecności NAA uzyskano największą liczbę korzeni, lecz były one najkrótsze. Ukorzenione pędy najlepszej jakości otrzymano na pożywce uzupełnionej 0,5 mg·dm⁻³ IAA. Rośliny przenoszone do podłoża do warunków ex vitro przyjmowały się w 70%.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2013
• Tom: 12
• Numer: 5
• Opis fizyczny: p.193-206,fig.,ref,

Twórcy

autor

Stelmaszczuk M.

• Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, 58 Leszczynskiego Str. 20-068 Lublin, Poland

autor

Kozak D.

• Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, 58 Leszczynskiego Str. 20-068 Lublin, Poland

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