Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species

• Autorzy: Singh S.K. , Rai M. K. , Asthana P. , Pandey S. , Jaiswal V. S. , Jaiswal U.
• Języki publikacji: EN

Abstrakty

EN

This article demonstrates the plantlet regeneration from alginate-encapsulated shoot tips of Spilanthes acmella. Shoot tip explants excised from in vitro proliferated shoots were encapsulated in calcium alginate beads. The best gel complexation for encapsulation of shoot tips was achieved using 3% sodium alginate and 100 mM calcium chloride. Maximum percent response for the conversion of encapsulated shoot tips into plantlets was obtained on growth regulator-free full-strength liquid MS (Murashige and Skoog, Physiol Plant 15:473–497, 1962) medium. The addition of MS nutrients in alginate matrix was found to have pronounced effect on shoot and root emergence from alginate beads. Encapsulated shoot tips could be stored at low temperature (4°C) up to 60 days. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully. The present synthetic seed technology could be useful in large-scale propagation as well as short-term conservation and germplasm distribution and exchange of Spilanthes acmella.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 3
• Opis fizyczny: p.649-653,fig.,ref.

Twórcy

autor

Singh S.K.

• Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India
• Department of Life Science, Institute of Plant Biology, National Taiwan University, Taipei, Taiwan

autor

Rai M. K.

• Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India

autor

Asthana P.

• Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India

autor

Pandey S.

• Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India
• Department of Botany, Laboratory of Algal Biology, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India

autor

Jaiswal V. S.

• Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India

autor

Jaiswal U.

• Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India

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