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2014 | 17 | 2 |
Tytuł artykułu

Assessment of serum levels of allergen-specific immunoglobulin E in different seasons and breeds in healthy horses

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The present study was designed to asses specific IgE towards environment allergens in 42 healthy horses. Determination of this immunoglobulin in serum serve as diagnostic tools in allergic diseases to improve efficacy of the treatment and proper allergen selection to specific immunotherapy. Serum levels of allergen specific IgE were measured with equine monoclonal antibody, using 15 individual and 5 mix allergens in North European Panel. The study revealed season dependent increased levels of allergen specific IgE in normal horses. It is noteworthy that healthy horses show high percentage of positive reactions, most commonly towards to domestic mites D. farinae (80%), D. pteronyssinus (35.71%) and storage mites T. putrenscentiae (42.86%), Acarus siro (40.48%). These allergens play an important role in equine, canine and feline atopic dermatitis. We also demonstrated high IgE levels in the group of horse specific insect allergens. Tabanus sp. (35.71%), Culicoides sp. (28.57%) and Simulium sp. (26.19%) were the most frequent insect positive reaction allergens. No positive reactions in all groups of allergens were found in winter season, low and merely detectable levels of antibodies have been found relating to D. farianae and T. putrescentiae allergen. We observed elevated mould-IgE levels in horses that live in stables, while outdoor living horses showed very low levels. Amongst all positive reactions we observed only weak and moderate reactions but no strong positive reactions were found. No significant differences were observed between three breeds of horses with the exception of moulds and D. pteronyssinus allergens.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
17
Numer
2
Opis fizyczny
p.331-337,fig.,ref.
Twórcy
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
autor
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
autor
  • Equine Breeding and Management Unit, Department of Biology and Animal Breeding, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
autor
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
autor
  • Sub-Department of Internal Diseases of Farm Animals and Horses, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
autor
  • Agromarina Farm, Kulczyn-Kolonia 48, 22-235 Hansk Pierwszy, Poland
autor
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
Bibliografia
  • Eder C, Curik I, Brem G, Crameri R, Bodo I, Habe F, Lazary S, Sölkner J, Marti E (2001) Influence of environmental and genetic factors on allergen-specific immunoglobulin- E levels in sera from Lipizzan horses. Equine Vet J 33: 714-720.
  • Frey R, Bergvall K, Egenvall A (2008) Allergen-specific IgE in Icelandic horses with insect bite hypersensitivity and healthy controls, assessed by FcεR1α-based serology. Vet Immunol Immunopathol 126: 102-109.
  • Hellberg W, Wilson AD, Mellor P, Doherr MG, Torsteinsdottir S, Zurbriggen A, Jungi T, Marti E (2006) Equine insect bite hypersensitivity: immunoblot analysis of IgE and IgG subclass responses to Culicoides nubeculosus salivary gland extract. Vet Immunol Immunopathol 113: 99-112.
  • Kalina WV, Pettigrew HD, Gershwin LJ (2003) IgE ELISA using antisera derived from epsilon chain antigenic peptides detects allergen-specific IgE in allergic horses. Vet Immunol Immunopathol 92: 137-147.
  • Meulenbroeks C, van der Meide NM, Zaiss DM, van Oldruitenborgh-Oosterbaan MM, van der Lught JJ, Smak J, Rutten VP, Willemse T (2013) Seasonal differences in cytokine expression in the skin of Shetland ponies suffering from insect bite hypersensitivity. Vet Immunol Immunopathol 151: 147-156.
  • Morgan EE, Miller WH Jr, Wagner BA (2007) A comparison of intradermal testing and detection of allergen-specific immunoglobulin E in serum by enzyme-linked immunosorbent assay in horses affected with skin hypersensitivity. Vet Immunol Immunopathol 120: 160-167.
  • Petersen A, Schott HC (2009) Effects of dexamethasone and hydroxyzine treatment on intradermal testing and allergen- specific IgE serum testing results in horses. Vet Dermatol 20: 615-622.
  • Roque JB, O’Leary CA, Kyaw-Tanner M, Latter M, Mason K, Shipstone M, Vogelnest L, Duffy D (2011) High allergen- specific serum immunoglobulin E levels in nonatopic West Highland white terriers. Vet Dermatol 22: 257-266.
  • Steinman A, Peer G, Klement E (2003) Epidemiological study of Culicoides hypersensitivity in horses in Israel. Vet Rec 152: 748-751.
  • Wagner B, Miller WH, Morgan EE, Hillegas JM, Erb HN, Leibold W, Antczak DF (2006) IgE and IgG antibodies in skin allergy of the horse. Vet Res 37: 813-825.
  • Wagner B, Miller WH Jr, Erb HN, Lunn DP, Antczak DF (2009) Sensitization of skin mast cells with IgE antibodies to Culicoides allergens occurs frequently in clinically healthy horses. Vet Immunol Immunopathol 132: 53-61.
  • Wilson AD, Harwood LJ, Bjo¨rnsdottir S, Marti E, Day MJ (2001) Detection of IgG and IgE serum antibodies to Culicoides salivary gland antigens in horses with insect dermal hypersensitivity (sweet itch). Equine Vet J 33: 707-713.
  • Wilson AD, Harwood L, Torsteinsdottir S, Marti E (2006) Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandic and non-Icelandic horses with insect bite dermal hypersensitivity. Vet Immunol Immunopathol 112: 156-170.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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