Cloning and characterization of a thaumatin-like protein gene PeTLP in Populus deltoides 3 P. euramericana cv. ‘Nanlin895’

• Autorzy: Wang L. , Yang L. , Zhang J. , Dong J. , Yu J. , Zhou J. , Zhuge Q.
• Języki publikacji: EN

Abstrakty

EN

Thaumatin-like protein (TLP) genes constitute a large, highly complex gene family involved in host defense against abiotic stresses, as well as other physiological processes. However, their functions in the abiotic stress-induced defense response in poplar remain largely unknown. In this study, one full-length cDNA from a hybrid poplar (Populus deltoides 9 P. euramericana cv. ‘Nanlin895’) that encodes a TLP was isolated by RACE and designated PeTLP. The deduced amino acid sequence of PeTLP encodes a protein of a predicted molecular mass 24.5 kDa and a pI of 8.19. Transcript of PeTLP accumulated in stems, but the gene showed low transcriptional levels in roots and leaves. PeTLP was upregulated in leaves after abiotic treatments, including 200 mM NaCl, 10 % PEG6000, 100 lM gibberellin, 1 mM salicylic acid and 100 lM abscisic acid. Subcellular localization analysis showed that GFP-tagged PeTLP predominantly localizes as discrete points in the cytoplasm and the periphery of the plasma membrane. Recombinant PeTLP obtained using Escherichia coli expression produced antifungal activities to Rhizopus sp., Aspergillus niger and Penicillium citrinum. These results suggest that PeTLP is an abiotic stresses- induced protein and plays roles in physiological processes of poplar development, and is involved in the defense of poplar against abiotic stresses. Manipulation of PeTLP in poplar or other trees should improve their performance under various abiotic stress conditions.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 10
• Opis fizyczny: p.2985-2998,fig.,ref.

Twórcy

autor

Wang L.

• Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

autor

Yang L.

• Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

autor

Zhang J.

• Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

autor

Dong J.

• Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

autor

Yu J.

• Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

autor

Zhou J.

• Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

autor

Zhuge Q.

• Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Bibliografia

• Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Campos MA, Silva MS, Magalhães CP, Ribeiro SG, Sarto RPD, Vieira EA, Grossi de Sá MF (2008) Expression in Escherichia coli, purification, refolding and antifungal activity of an osmotin from Solanum nigrum. Microb Cell Fact 7:7
• Chan YW, Tung WL, Griffith M, Chow KC (1999) Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization. J Exp Bot 50:1627–1628
• Dafoe NJ, Gowen BE, Constabel CP (2010) Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar. BMC Plant Biol 10:191–201
• Eden L, Hesling L, Klock R, Ledeboer AM, Meat J, Toonen MY, Visser C, Verrips CY (1982) Cloning of cDNA encoding the sweet tasting plant protein thaumatin and its expression in Escherichia coli. Gene 18:1–12
• Fils-Lycaon BR, Wiersma PA, Eastwell KC, Sautiere P (1996) A cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like. Plant Physiol 111:269–273
• Garcia-Casado G, Collada C, Allona I, Soto A, Casado R, Rodriguez-Cerezo E, Gomez L, Aragoncillo C (2000) Characterization of an apoplastic basic thaumatin-like protein from recalcitrant chestnut seeds. Physiol Plantarum 110:172–180
• Grenier J, Potvin C, Trudel J, Asselin A (1999) Some thaumatin-like proteins hydrolyse polymeric b-1, 3-glucans. Plant J 19:473–480
• Jami SK, Swathi Anuradha T, Guruprasad L, Kirti PB (2007) Molecular, biochemical and structural characterization of osmotin-like protein from black nightshade (Solanum nigrum). J Plant Physiol 164:238–252
• Jayasankar S, Li Z, Gray DJ (2003) Constitutive expression of Vitis vinifera thaumatin-like protein after in vitro selection and its role in anthracnose resistance. Funct Plant Biol 30:1105–1115
• Jung YC, Lee HJ, Yum SS, Soh WY, Cho DY, Auh CK, Lee TK, Soh HC, Kim YS, Lee SC (2005) Drought-inducible-but ABAindependent-thaumatin-like protein from carrot (Daucus carota L.). Plant Cell Rep 24:366–373
• Kenton P, Darby RM, Shelley G, Draper J (2000) A PR-5 gene promoter from Asparagus officinalis (AoPRT-L) is not induced by abiotic stress, but is activated around sites of pathogen challenge and by salicylate in transgenic tobacco. Mol Plant Pathol 1:367–378
• Kim YS, Park JY, Kim KS, Ko MK, Cheong SJ, Oh BJ (2002) A thaumatin-like gene in nonclimacteric pepper fruits used as molecular marker in probing disease resistance, ripening, and sugar accumulation. Plant Mol Biol 49:125–135
• Kim BG, Fukumoto T, Tatano S, Gomi K, Ohtani K, Tada Y, Akimitsu K (2009) Molecular cloning and characterization of a thaumatin-like protein-encoding cDNA from rough lemon. Physiol Mol Plant Pathol 74:3–10
• Koiwa H, Kato H, Nakatsu T, Oda J, Yamada Y, Sato F (1999) Crystal structure of tobacco PR-5d protein at 1.8 A resolution reveals a conserved acidic cleft structure in antifungal thaumatin-like proteins. J Mol Biol 286:1137–1145
• Krebitz M, Wagner B, Ferreira F, Peterbauer C, Campillo N, Witty M, Kolarich D, Steinkellner H, Scheiner O, Breiteneder H (2003) Plant-based heterologous expression of Mal d 2, a thaumatin-like protein and allergen of apple (Malus domestica), and its characterization as an antifungal protein. J Mol Biol 329: 721–730
• Kuwabara C, Takezawa D, Shimada T, Hamada T, Fujikawa S, Arakawa K (2002) Abscisic acid- and cold-induced thaumatinlike protein in winter wheat has antifungal activity against snow mould, Microdochium nivale. Physiol Plant 115:101–110
• Léon-Kloosterziel KM, Verhagen BW, Keurentjes JJ, VanPelt JA, Rep M, VanLoon LC, Pieterse CM (2005) Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle. Plant Mol Biol 57:731–748
• Linthorst HJM (1991) Pathogenesis related proteins of plants. CRC Crit Rev Plant Sci 10:123–150
• Liu JJ, Zamani A, Ekramoddoullah AKM (2010a) Expression profiling of a complex thaumatin-like protein family in western white pine. Planta 231:637–651
• Liu JJ, Sturrock R, Ekramoddoullah AKM (2010b) The superfamily of thaumatin-like proteins: its origin, evolution, and expression towards biological function. Plant Cell Rep 29:419–436
• Liu DQ, He X, Li WX, Chen CY, Ge F (2012) Molecular cloning of a thaumatin-like protein gene from Pyrus pyrifolia and overexpression of this gene in tobacco increased resistance to pathogenic fungi. Plant Cell Tiss Organ Cult. doi:10.1007/s11240-012-0167-0
• Malehorn DE, Borgmeyer JR, Smith CE, Shah DM (1994) Characterization and expression of an antifungal zeamatin-like protein (Zlp) gene from Zea mays. Plant Physiol 106:1471–1481
• Nicholas KB, Nicholas HB Jr (1997) GeneDoc: a tool for editing and annotating multiple sequence alignments. http://www.psc.edu/biomed/genedoc
• Onishi M, Tachi H, Kojima T, Shiraiwa M, Takahara H (2006) Molecular cloning and characterization of a novel salt-inducible gene encoding an acidic isoform of PR-5 protein in soybean (Glycine max [L.] Merr.). Plant Physiol Biochem 44:574–580
• Osmond RIW, Hrmova M, Fontaine F, Imberty A, Fincher GB (2001) Binding interactions between barley thaumatin-like proteins and (1, 3)-b-D-glucans. Eur J Biochem 15:4190–4199
• Petre B, Major L, Roubier N, Duplessis S (2011) Genome-wide analysis of eukaryote thaumatin-like proteins (TLPs) with an emphasis on poplar. BMC Plant Biol 11:33–48
• Rajam MV, Chandola N, Goud PS, Singh D, Kashyap V, Choudhary ML, Sihachakr D (2007) Thaumatin gene confers resistance to fungal pathogens as well as tolerance to abiotic stresses in transgenic tobacco plants. Biol Plant 51:135–141
• Rinaldi C, Kohler A, Frey P, Duchaussoy PFF, Ningre N, Couloux A, Wincker P, Thiec DL, Fluch S, Martin F, Duplessis S (2007) Transcript profiling of poplar leaves upon infection with compatible and incompatible strains of the foliar rust Melampsore larici-populina. Plant Physiol 144:347–366
• Sakamoto Y, Watanabe H, Nagai M, Nakade K, Takahashi M, Sato T (2006) Lentinula edodes tlg1 encodes a thaumatin-like protein that is involved in lentinan degradation and fruiting body senescence. Plant Physiol 141:793–801
• Sassa H, Hirano H (1998) Style-specific and developmentally regulated accumulation of a glycosylated haumatin/PR5-like protein in Japanese pear (Pyrus serotina Rehd.). Planta 205:514–521
• Sassa H, Ushijima K, Hirano H (2002) A pistil-specific thaumatin/PR5-like protein gene of Japanese pear (Pyrus erotina): sequence and promoter activity of the 50 region in transgenic tobacco. Plant Mol Biol 50:371–377
• Schestibratov KA, Dolgov SV (2005) Transgenic strawberry plants expressing a thaumatin II gene demonstrate enhanced resistance to Botrytis cinerea. Sci Hortic 106:177–189
• Schultz J, Copley RR, Doerks T, Ponting CP, Bork P (2000) SMART: a web-based tool for the study of genetically mobile domains. Nucleic Acids Res 28:231–234
• Skadsen RW, Sathish P, Kaeppler HF (2000) Expression of thaumatin-like permatin PR-5 genes switches from the ovary wall to the aleurone in developing barley and oat seeds. Plant Sci 156:11–22
• Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
• Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882
• Trudel J, Grenier J, Potvin C, Asselin A (1998) Several thaumatinlike proteins bind to 1,3-glucans. Plant Physiol 118:1431–1438
• Tuskan GA, DiFazio S, Jansson S et al (2006) The genome of black cottonwood, Populus trichocarpa (Tott. & Gray). Science 313:1596–1604
• Van Damme EJ, Charels D, Menu-Bouaouiche L, Proost P, Barre A, Rougé P, Peumans WJ (2002) Biochemical, molecular and structural analysis of multiple thaumatin-like proteins from the elderberry tree (Sambucus nigra L.). Planta 214:853–862
• Velazhahan R, Datta SK, Muthukrishnan S (1999) The PR-5 family: thaumatin-like proteins in plants. In: Datta SK, Muthukrishnan S (eds) Pathogenesis-related proteins in plants. CRC Press, Boca Raton, pp 107–129
• Yang X, Kalluri UC, DiFazio SP, Wullschleger SD, Tschaplinski TJ, Cheng ZM, Tuskan GA (2009) Poplar genomics: state of the science. Crit Rev Plant Sci 28:285–308
• Ye XY, Wang HX, Ng TB (1999) First chromatographic isolation of an antifungal thaumatin-like protein from French bean legumes and demonstration of its antifungal activity. Biochem Biophys Res Comm 263:1002–1013
• Zhao JP, Su XH (2010) Patterns of molecular evolution and predicted function in thaumatin-like proteins of Populus trichocarpa. Planta 232:949–962

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