Virulence genes profiles and phylogenetic origin of Escherichia coli from acute and chronic intestinal diseases revealed by comparative genomic hybridization microarray

• Autorzy: Sobieszczanska B. , Kasprzykowska U. , Turniak M. , Maciejewski H. , Franiczek R. , Duda -Madej A.
• Języki publikacji: EN

Abstrakty

EN

The association between Escherichia coli virulence factors and chronic intestinal disorders is mostly unknown. The presented study compared the distribution of virulence genes and phylogroups among E. coli isolated from chronic intestinal disorders such as Crohn’s disease and irritable bowel syndrome (IBS) with strains isolated from patients with acute diarrhea as a control group. The presence of 159 virulence genes corresponding to known E. coli pathotypes was determined among 78 E. coli archive strains isolated from IBS, acute diarrhea and Crohn’s disease using CGH microarray. E. coli isolated from IBS demonstrated a mosaic of virulence genes specific to enteropathogenic, enterotoxigenic, enterohemorrhagic E. coli strains and Shigella species. In contrast, virulence factors and phylogroups distribution among E. coli isolated from children with acute diarrhea was similar to extraintestinal E. coli strains that probably acquired some virulence genes. The acquisition of virulence genes might have an impact on diarrheagenic potential of these strains. On the other hand, E. coli isolated from children with Crohn’s disease seem to be similar to adherent-invasive E. coli strains (AIEC), as it lack most known virulence genes. The presented study showed that these analyzed groups of E. coli strains differed from each other with the respect to the distribution of virulence genes. The differences in gene content support the idea that the participation of E. coli in chronic intestinal diseases is mostly related to virulence potential of these strains.

Słowa kluczowe

EN

virulence gene; virulence factor; gene; phylogenetic origin; Escherichia coli; acute intestinal disease; chronic intestinal disease; intestine disease; Crohn's disease; irritable bowel syndrome; comparative genomic hybridization microarray; DNA microarray

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2012
• Tom: 61
• Numer: 2
• Opis fizyczny: p.105-110,fig.,ref.

Twórcy

autor

Sobieszczanska B.

• Department of Microbiology, University of Medicine, Chalubinskiego 4 Str., 50-368 Wroclaw, Poland

autor

Kasprzykowska U.

autor

Turniak M.

autor

Maciejewski H.

autor

Franiczek R.

autor

Duda -Madej A.

Bibliografia

• Abdel-Hady M. and S.K. Bunn. 2004. Infjammatory bowel disease. Curr. Pediatr. 14: 598–604.
• Afset J.E., G. Bruant, R. Brousseau, J. Harel, E. Anderssen, L. Bevanger and K. Bergh. 2006. Identification of virulence genes linked with diarrhea due to atypical enteropathogenic Escherichia coli by DNA microarray and PCR. J. Clin. Microbiol. 44: 3703–3711.
• Benchimol E.I., A. Guttmann, A.M. Griffiths, L. Rabeneck, D.R. Mack, H. Brill, J. Howard, J. Guan and T. To. 2009. Increasing incidence of paediatric inflammatory bowel disease in Ontario, Canada: evidence from health administrative data. Gut 58: 1490–1497.
• Bruant G., C. Maynard, S. Bekal, I. Gaucher, L. Masson, R. Brous- seau and J. Harel. 2006. Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli. Appl. Environ. Microbiol. 72: 3780–3784.
• Clermont O., S. Bonacrosi and E. Bingen. 2000. Rapid and simple determination of the Escherichia coli phylogenetic groups. Appl. Environ. Microbiol. 66: 4555–4558.
• Darfeuille-Michaud A., J. Boudeau, P. Bulois, C. Neut, A.L. Glasser, N. Barnich, M.A. Bringer, A. Swidsinski, L. Beaugerie and J.F. Colombel. 2004. High prevalence of adherent-invasive Escherichia coli associated with ileal mucosa in Crohn’s disease. Gastroenterol. 127: 412–421.
• Doughty S., J. Sloan, V. Bennet-Wood, M. Robertson, R.M. Robins- Browne and E. Hartland. 2002. Identification of a novel fimbrial gene cluster related to long polar fimbriae in locus of enterocyte effacement-negative enterohemorrhagic strains of Escherichia coli. Infect. Immun. 70: 6761–6769.
• Duriez P., O. Clermont, S. Bonacorsi, E. Bingen, A. Chaventre, J. Elion, B. Picard and E. Denamur. 2001. Commensal Escherichia coli isolates are phylogentically distributed among geographically distinct populations. Microbiol. 147: 1671–1676.
• Garcia-Rodrigez L.A. and A. Ruigomez. 1999. Increased risk of irritable bowel after bacterial gastroenteritis: cohort study. BJM 318: 565–566.
• Gilson L., H.K. Mahanty and R. Kolter. 1987. Four plasmid genes are required for colicin V synthesis, export, and immunity. J. Bacteriol. 169: 2466–2470.
• Huang D.B., A. Mohanty, H.L. DuPont, P.C. Okhuysen and T. Chaing. 2006. A review of an emerging enteric pathogen: enteroaggregative Escherichia coli. J. Med. Microbiol. 55: 1303–1311.
• Hunter P.R. 2003. Drinking water and diarrheal disease due to Esche richia coli. J. Water Health 1: 65–72.
• Ishii S., K.P. Meyer and M.J. Sadowsky. 2007. Relationship between phylogenetic groups, genotypic clusters, and virulence gene profiles of Escherichia coli strains from diverse human and animal sources. Appl. Environ. Microbiol. 73: 5703–5710.
• Kaper J.B., J.P. Nataro and H.L. Mobley. 2004. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2: 123–140.
• Kaur P., A. Chakraborti and A. Asea. 2010. Enteroaggregative Escherichia coli: an emerging enteric food borne pathogen. Interdiscip. Perspect. Infect. Dis. 2010: 254159.
• Kotlowski R., C.N. Bernstein, S. Sepehri and D.O. Krause. 2007. High prevalence of Escherichia coli belonging to the B2 and D phylogenetic groups in inflammatory bowel disease. Gut 56: 669–675.
• Krotkova N., E. Cota, Y. Lebedin, S. Monpouet, J. Guignot, A.L. Servin, S. Matthews and S.L. Moseley. 2006. A subfamily of Dr adhesins of Escherichia coli bind independently to Decay-accelerating factor and the N-domain of carcinoembryogenic antigen. J. Biol. Chem. 281: 29120–29130.
• Martinez-Medina M., A. Mora, M. Blanco, C. Lopez, M. Pilar Alonso, S. Bonacorsi, M.H. Nicolas-Chanoine, A. Darfeuille-Michaud, J. Garcia-Gil and J. Blanco. 2009. Similarity and divergence among adherent-invasive Escherichia coli and extraintestinal pathogenic E. coli strains. J. Clin. Microbiol. 47: 3968–3979.
• Millies J.L., F. Navarro-Garcia, I.N. Okeke, J. Frederickson, J.P. Nataro and J.B. Kaper. 2001. EspC pathogenicity island of enteropathogenic Escherichia coli encodes an enterotoxin. Infect. Immun. 69: 315–324.
• Moyer S.M. 2005. A collaborative effort to define the epidemiology of pediatric inflammatory bowel disease: what can we learn from children with early-onset disease? J. Pediatr. 146: 7–8.
• Nowrouzian F., I. Adlerberth and A.E. Wold. 2001. P fimbriae, capsule and aerobactin characterize colonic resident Escherichia coli. Epidemiol. Infect. 126: 11–18.
• Ohman L. and M. Simren. 2007. New insights into the pathogenesis and pathophysiology of irritable bowel syndrome. Dig. Liver Dis. 39: 201–215.
• Okeke I.N. and J.P. Nataro. 2001. Enteroaggregative Escherichia coli. Lancet Infect. Dis. 1: 304–313.
• Olden K.W. 2003. The challenge of diagnosis irritable bowel syndrome. Rev. Gastroenterol. Disord. 3 Suppl. 3: S3–11.
• Östblom A., I. Adlerberth, A.E. Wold and F. Nowrouzian. 2011. Pathogenicity island markers, virulence determinants malX and usp, and the capacity of Escherichia coli to persist in infant’s commensal microbiotas. Appl. Environ. Microbiol. 77: 2303–2308.
• Paciorek J. 2002. Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhea. J. Med. Microbiol. 51: 548–556.
• Petersen A.M., E.M. Niessen, E. Litrup, J. Brynskov, A. Mirsepasi and K.A. Krogfelt. 2009. A phylogenetic group of Escherichia coli associated with active left-sided inflammatory bowel disease. BMC Microbiol. 9: 171.
• Rolhion N. and A. Darfeuille-Michaud. 2007. Adherent-invasive Escherichia coli in inflammatory bowel disease. Inflamm. Bowel Dis. 13: 1277–1283.
• Rolland K., N. Lambert-Zechvsky, B. Picard and E. Denamur. 1998. Shigella and enteroinvasive Escherichia coli strains are derived from distinct ancestral strains of E. coli. Microbiol. 144: 2667–2672.
• Sahl J.W., H. Steinsland, J.C. Redman, S. V. Angiuoli, J.P. Nataro, H. Sommerfelt and D.A. Rasko. 2011. A comparative genomic analysis of diverse types of enterotoxigenic Escherichia coli reveals pathovar-specific conservation. Infect. Immun. 79: 950–960.
• Sobieszczanska B.M., J. Osek, D. Wasko-Czopnik, E. Dworniczek and K. Jermakow. 2007. Association of enteroaggregative Escherichia coli with irritable bowel syndrome. Clin. Microbiol. Infect. 13: 404–407.
• Turner S.M., R.R. Chaudhuri, Z. D. Jiang, H. DuPont, C. Gyles, C.W. Pensi, M.J. Pallen and I.R. Henderson. 2006. Phylogenetic comparisons reveal multiple acquisition of the toxin gene by enterotoxigenic Escherichia coli strains of different evolutionary lineages. J. Clin. Microbiol. 44: 4528–4536.
• Vidal J.E. and F. Navarro-Garcia. 2006. Efficient translocation of EspC into epithelial cells depends on enteropathogenic Escherichia coli and host cell contact. Infect Immun. 74: 2293–2303.
• Weintraub A. 2007. Enteroaggregative Escherichia coli: epidemiology, virulence and detection. J. Med. Microbiol. 56: 4–8.
• Zhang L., B. Foxman, J.R. Gilsdorf and C.F. Marrs. 2005. Bacterial genomic DNA isolation using sonication for microarray analysis. BioTechiques 39: 640–644