Determination of riboflavin in premixture and compound feed by liquid chromatography method

• Autorzy: Rubaj J. , Bielecka G. , Korol W. , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

The analytical procedure of riboflavin quantification in premixtures and compound feedingstuffs by high performance liquid chromatography (HPLC) analysis is presented. Riboflavin was extracted from feed sample in autoclave with 0.1M sulphuric acid. The ester bonds with phosphoric acid were hydrolysed by the Taka-diastase enzyme. Riboflavin content was determined by HPLC with reversed-phase and usage of fluorescence detector. The limit of quantification of this method is 1.0 mg/kg. The coefficient of variation of riboflavin quantification results in premixtures was 2.29% and in compound feedingstuffs - 3.39%. The Horrat value for premixtures reached 0.61, whereas in case of compound feedingstuffs - 0.40, thus confirming an acceptable precision of the applied procedure. The recovery rate for riboflavin added in the form of a reference solution into premixtures was 98.3%, as regards compound feedingstuffs it was 98.0%.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 4
• Opis fizyczny: p.619-624,fig.,ref.

Twórcy

autor

Rubaj J.

• National Feed Laboratory, National Research Institute of Animal Production, 20-079 Lublin, Poland

autor

Bielecka G.

• National Feed Laboratory, National Research Institute of Animal Production, 20-079 Lublin, Poland

autor

Korol W.

• National Feed Laboratory, National Research Institute of Animal Production, 20-079 Lublin, Poland

autor

Kwiatek K.

• National Veterinary Research Institute, 24-100 Pulawy, Poland

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