Short‑term exposure to high‑intensity sound induces hearing loss and apoptosis in guinea pigs

• Autorzy: Liu Y. , Wang H. , Liu Z. , Gu Y. , Xin L. , Liu J. , Fan H.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate the effect and underlying mechanisms of short-term high-intensity sound exposure on guinea pigs to mimic the effects of non-lethal anti-riot weapons. A total of 92 male adult guinea pigs were exposed to high-intensity sound at 0 dB, 110 dB and 130 dB for 5 min. Basic clinical observation, repellent behaviour detection, peripheral blood routine examination, serum cortisol detection and hearing ability assessment were performed to analyse the functional changes after high intensity sound exposure. Meanwhile, routine haematoxylin and eosin staining, scanning electron microscopy and transmission electron microscopy were used to observe the structure of the cochlear tissue. To investigate the mechanisms underlying the tissue changes, the levels of apoptosis and caspase 3, 8 and 9 were detected using TUNEL staining and immunohistochemistry. After short-term exposure to high-intensity sound, the guinea pigs exhibited fear and agitation, increased repulsive behaviour, high serum cortisol and an increase in auditory threshold. The inner hair cells and outer hair cells exhibited degeneration. In addition, apoptosis was observed in the cochlear tissue. After short-term exposure to high-intensity sound, the guinea pigs exhibited not only stress reactions but also impaired hearing and signs of hair cell degeneration. Apoptosis in the cochlear tissue may play an important role in the functional and structural injuries caused by high-intensity sound.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2019
• Tom: 79
• Numer: 2
• Opis fizyczny: p.155-168,fig.,ref.

Twórcy

autor

Liu Y.

• Institute of Disaster Medicine, Tianjin University, Tianjin, China
• Department of Medical Service, Logistics University of Chinese People’s Armed Police Forces, Tianjin, China

autor

Wang H.

• Department of Experimental Pathology, Institute of Radiation Medicine, Academy of Military Sciences, Beijing, China

autor

Liu Z.

• Institute of Disaster Medicine, Tianjin University, Tianjin, China

autor

Gu Y.

• Department of Medical Service, Logistics University of Chinese People’s Armed Police Forces, Tianjin, China

autor

Xin L.

• Institute of Disaster Medicine, Tianjin University, Tianjin, China

autor

Liu J.

• Institute of Disaster Medicine, Tianjin University, Tianjin, China

autor

Fan H.

• Institute of Disaster Medicine, Tianjin University, Tianjin, China

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Identyfikatory

DOI

10.21307/ane‑2019‑014