Analysis of the neurogenic potential of human umbilical cord blood neural stem cells (HUCB-NSC) after their transplantation into rat brain

• Autorzy: Kozlowska H. , Markiewicz I. , Jablonska A. , Habich A. , Zychowicz M. , Wanacka E. , Domanska-Janik K. , Lukomska B.
• Języki publikacji: EN

Abstrakty

EN

Transplantation (tx) of neural stem cells (NSC) is the key strategy of cell replacement therapy in the central nervous system. The goal of the study was to compare survival, migration and differentiation of HUCB-NSC after their tx into the brain of neonatal and adult Wistar rats. Methods: HUCB-NSC (2 × 104 ) labeled with CMFDA were tx into SVZ of the postnatal day 0 (P0) rats or into intact brain of adult rats. After 1, 3, 7, 14 or 21 days brains were removed, frozen and cut into 20 μm coronary slices, then immunohistochemical studies were performed to visualize HUCB-NSC fate in the brain. Results: In neonatal rats, 3 days after tx most of HUCB-NSC remained in the graft. During the fi rst week HUCB-NSC started to disperse and migrate. HUCB-NSC situated at the periphery of the graft or in migratory stream display proliferation marker (Ki67). After 7ñ21 days HUCB-NSC survived in the host brain with many cells expressing neuronal or astrocytic phenotypes. Few of HUCB-NSC presented the features of adult neurons (MAP2+ with long protrusions). In adult rats, 3 days after tx, HUCB-NSC form dense deposit with single cells migrating into brain tissue. Most of the HUCB-NSCs stayed undifferentiated with few cells expressing neuronal (NF200) or astrocytic (GFAP) markers. After 7 days numerous HUCB-NSC situated inside the graft underwent degeneration and subsequent depletion. Tx HUCB-NSC induced infl ammatory response detected by macrophage/microglia (ED1+) accumulation and astrogliosis (GFAP+). No viable HUCB-NSC were found after 14 days. Conclusions: Host environment dictates the fate of tx neural stem cells derived from human cord blood however immunological response in adult rats limits the time of observation due to short survival of HUCB-NSC. Supported by MSHE grant no 2PO5A05430

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Opis fizyczny: p.107

Twórcy

autor

Kozlowska H.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Markiewicz I.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Jablonska A.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Habich A.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Zychowicz M.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Wanacka E.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Domanska-Janik K.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Lukomska B.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland