Invasiveness of Listeria monocytogenes strains isolated from animals in Poland

• Autorzy: Walecka-Zacharska E. , Kosek-Paszkowska K. , Bania J. , Staroniewicz Z. , Bednarski M. , Wieliczko A.
• Języki publikacji: EN

Abstrakty

EN

Animals are important reservoir of Listeria monocytogenes, a pathogen causing serious infections in both humans and livestock. However, data on invasiveness of L. monocytogenes strains of animal origin is very scarce. Ability of 18 L. monocytogenes strains of animal origin to invade HT- 9 cells was investigated. Plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. Almost 40% of L. monocytogenes strains were weakly invasive. It was shown that strains from serogroup 4b exhibited the highest invasiveness, whereas serogroup 1/2b consisted of strains of invasiveness below 0.0001%. Analysis of translated inlA and inlB gene sequences revealed no premature stop codons. Lineage-specific mutations in low invasive strains were identified within inlA and inlB sequences. Our results demonstrate high incidence of low invasive animal L. Monocytogenes strains, which may be at least partly explained by unique point mutations in the InlA and InlB.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 4
• Opis fizyczny: p.697-702,ref.

Twórcy

autor

Walecka-Zacharska E.

• Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375, Wroclaw, Poland

autor

Kosek-Paszkowska K.

• Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375, Wroclaw, Poland

autor

Bania J.

• Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375, Wroclaw, Poland

autor

Staroniewicz Z.

• Department of Pathology, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375, Wroclaw, Poland

autor

Bednarski M.

• Department of Epizootiology with Clinic for Birds and Exotic Animals, pl. Grunwaldzki 45, 50-366, Wroclaw, Poland

autor

Wieliczko A.

• Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375, Wroclaw, Poland

Bibliografia

• Bania J, Żarczyńska A, Molenda J, Dabrowska A, Kosek-Paszkowska K, Wieckowska-Szakiel M, Rózalska B (2009) Subtyping of Listeria monocytogenes isolates by actA gene sequencing, PCR-fingerprinting, and cell-invasion assay. Folia Microbiol 54: 17-24.
• Barbour AH, Rampling A, Hormaeche CE (2001) Variation in the infectivity of Listeria monocytogenes isolates following intragastric inoculation of mice. Infect Immun 69: 4657-4660.
• Bierne H, Sabet C, Personnic N, Cossart P (2007) Internalins: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes. Microbes Infect 9: 1156-1166.
• Cartwright EJ, Jackson KA, Johnson SD, Graves LM, Silk BJ, Mahon BE (2013) Listeriosis outbreaks and associated food vehicles, United States, 1998-2008. Emerg Infect Dis 19: 1-9.
• Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P (2004) Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 42: 3819-3822.
• Farber JM, Peterkin PI (1991) Listeria monocytogenes, a food-borne pathogen. Microbiol Rev 55: 476-511.
• Glaser P, Frangeul L, Buchrieser C , Rusniok C, Amend A, Baquero F, Berche P, Bloecker H, Brandt P, Chakraborty T, Charbit A, Chetouan F, Couvé E, de Daruvar A, Dehoux P, Domann E, Domtnguez-Bernal G, Duchaud E, Durant L, Dussurget O, Entian KD, Fsihi H, Garcta-del Portillo F, Garrido P, Gautier L, Goebel W, Gómez-López N, Hain T, Hauf J, Jackson D, Jones LM, Kaerst U, Kreft J, Kuhn M, Kunst F, Kurapkat G, Madueno E, Maitournam A, Vicente JM, Ng E, Nedjari H, Nordsiek G, Novella S, de Pablos B, Pérez-Diaz JC, Purcell R, Remmel B, Rose M, Schlueter T, Simoes N, Tierrez A, Vázquez-Boland JA, Voss H, Wehland J, Cossart P (2001) Comparative genomics of Listeria species. Science 294: 849-852.
• Gray ML, Killinger AH (1966) Listeria monocytogenes and listeric infections. Bacteriol Rev 30: 309-382.
• Kim H, Marquis H, Boor KJ (2005) Sigma B contributes to Listeria monocytogenes invasion by controlling expression of inlA and inlB. Microbiology 151: 3215-3222.
• Kovačević J, Arguedas-Villa C, Wozniak A Tasara T, Allen KJ (2013) Examination of food chain-derived Listeria monocytogenes strains of different serotypes reveals considerable diversity in inlA genotypes, mutability, and adaptation to cold temperatures. Appl Environ Microbiol 79: 1915-1922.
• Nightingale KK, Windham K, Martin KE, Yeung M, Wiedmann M (2005) Select Listeria monocytogenes subtypes commonly found in foods carry distinct nonsense mutations in inlA, leading to expression of truncated and secreted internalin A, and are associated with a reduced invasion phenotype for human intestinal epithelial cells. Appl Environ Microbiol 71: 8764-8772.
• Nightingale KK, Ivy RA, Ho AJ, Fortes ED, Njaa BL, Peters RM, Wiedmann M (2008) inlA premature stop codons are common among Listeria monocytogenes isolates from foods and yield virulence-attenuated strains that confer protection against fully virulent strains. Appl Environ Microbiol 74: 6570-6583.
• Nightingale KK, Schukken YH, Nightingale CR, Fortes ED, Ho AJ, Her Z, Grohn YT, McDonough PL, Wiedmann M (2004) Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Appl Environ Microbiol 70: 4458-4467.
• Ragon M, Wirth T, Hollandt F, Lavenir R, Lecuit M, Le Monnier A, Brisse S (2008) A new perspective on Listeria monocytogenes evolution. PLoS Pathog 4: e1000146.
• Roche SM, Grepinet O, Kerouanton A, Ragon M, Leclercq A, Temoin S, Schaeffer B, Skorski G, Mereghetti L, Le Monnier A, Velge P (2012) Polyphasic characterization and genetic relatedness of low-virulence and virulent Listeria monocytogenes isolates. BMC Microbiol 12: 304.
• Roche SM, Gracieux P, Milohanic E, Albert I, Virlogeux-Payant I, Témoin S, Grépinet O, Kerouanton A, Jacquet C, Cossart P, Velge P (2005) Investigation of specific substitutions in virulence genes characterizing phenotypic groups of low-virulence field strains of Listeria monocytogenes. Appl Environ Microbiol 71: 6039-6048.
• Témoin S, Roche SM, Grépinet O, Fardini Y, Velge P (2008) Multiple point mutations in virulence genes explain the low virulence of Listeria monocytogenes field strains. Microbiology 154: 939-948.
• Vhzquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Domtnguez-Bernal G, Goebel W, Gonzhlez-Zorn B, Wehland J, Kreft J (2001) Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev 14: 584-640.
• Wałecka-Zacharska E, Kosek-Paszkowska K, Bania J, Karptšková R, Stefaniak T (2013) Salt stress-induced invasiveness of major Listeria monocytogenes serotypes. Lett Appl Microbiol 56: 216-221.
• Wesley IV, Larson DJ, Harmon KM, Luchansky JB, Schwartz AR (2002) A case report of sporadic ovine listerial menigoencephalitis in Iowa with an overview of livestock and human cases. J Vet Diagn Invest 14: 314-321.
• Wiedmann M, Bruce JL, Keating C, Johnson AE, McDonough PL, Batt CA (1997) Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infect Immun 65: 2707-2716.
• Zhou X, Jiao X, Wiedmann M (2005) Listeria monocytogenes in the Chinese food system: strain characterization through partial actA sequencing and tissue-culture pathogenicity assays. J Med Microbiol 54: 217-224.

Identyfikatory

DOI

10.1515/pjvs-2015-0090