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1988 | 39 | 3 |

Tytuł artykułu

Lipid peroxides, spermatozoa quality and activity of glutathione peroxidase in bull semen

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Slaweta, R., Laskowska, T. and Szymańska, E.: Lipid peroxides, spermatozoa quality and activity of glutathione peroxidase in bull semen. Acta physiol, pol., 1988, 30 (3): 207-214. In bull semen spontaneous lipid peroxidation measured by the level of endogenous lipid peroxides and the consequences of this process for morphological and biochemical changes was studied. Glutathione peroxidase activity as protective enzyme against peroxidative damage was also determined. Obtained results showed that approximately two thirds of GSH-Px activity in bull semen was non Se-dependent glutathione peroxidase activity. Malonaldehyde (MDA) level was negative correlated with selenium-dependent GSH-Px activity (r = -0.38, P < 0.01). Spermatozoa with acrosome entirely lost appeared to increase as the MDA level increased (r = 0.18, P < 0.05). The negative correlation between Se GSH-Px activity and spermatozoa with acrosome separation from head (r = -0.28, P< 0.0,1) and entirely lost (r = - 0.21, P < 0.05) suggest that selenium-dependent GSH-Px plays role in protecting the acrosome against disruption of the acrosomal membrane. The total glutathione peroxidase activity was unrelated to studied variables of bull semen.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

39

Numer

3

Opis fizyczny

p.207-214,ref.

Twórcy

autor
  • Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jablonna, Poland
autor
  • Department of Biochemistry, Institute of Biopharmacy, School of Medicine, Warsaw, Poland
autor
  • Department of Biochemistry, Institute of Biopharmacy, School of Medicine, Warsaw, Poland

Bibliografia

  • 1. Ahluwalia, В., Golman, R. T. (1969) Fatty acid composition of lipids of bull, boar, rabbit and human semen. J. Reprod. Pert. 18. 431-437.
  • 2. Alvarez, J. C., Storey, В. T. (1982) Spontaneous lipid peroxidation in rabbit epididymal spermatozoa: its effect on sperm motility. Biol. Reprod. 27. 1102-1108.
  • 3. Barber, A. A., Berheim, F. (1967) Lipid peroxidation: its measurement, occurrence and significance in animal tissues. Adv. Gerontol. Res. 2. 355-403.
  • 4. Bartle, J. L., Senger, P. L. (1980) Glutathione peroxidase and semen quality. J. Anim. Sci. (suppl. 1). 51. 258.
  • 5. Behne, D., Duk, M., Elger, W. (1986) Selenium content and glutathione peroxidase activity in the testis of the maturing rat. J. Nutr. 116. 1442-1447.
  • 6. Brown, D. V., Senger, P. L. (1977) Glutathione peroxidase in bovine ejaculate semen, seminal plasma and epididymal spermatozoa. Proc. 68th Ann. Meeting Amer. Sci. Anim. Sci. Abstr., p. 141.
  • 7. Buege, J. A., Aust, S. D. (1978) Microsomal lipid peroxidation. Methods Enzymol. 52. 302-310.
  • 8. Hopkins, J., Tudhope, G. R. (1973) Glutathione peroxidase in human red cells in health and disease. Brit. J. Haematolog. 25. 563-575.
  • 9. Jones, R., Mann, T. (1973) Lipid peroxidation in spermatozoa. Proc. R. Soc. Lond. В Biol. Sci. 184. 103-107.
  • 10. Jones, R., Mann, T. (1976) Lipid peroxidation in spermatozoa: formation, role of ptasmologen and physiological significance. Proc. R. Soc. В Biol. Sci. 193. 317-333.
  • 11. Jones, R., Mann, T., Sherins, R. J. (1978) Adverse effect of peroxidized lipid on human spermatozoa. Proc. R. Soc. Lond. В Biol. Sci. 201. 413-417.
  • 12. Jones, R., Mann, T., Sherins, R. J. (1979) Adverse effect of peroxidized of lipids in human spermatozoa, spermicidal properties of fatty acid peroxides and protective action of seminal plasma. Pert. Steril. 31. 531-537.
  • 13. Kappus, H. (1985) Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance, to Oxidation Stress. Ed. H. Siess, pp. 273-310, Academic Press.
  • 14. Lawrence, R. A., Burk, R. F. (1976) Glutathione peroxidase activity in selenium -deficient rat liver. Biochem. Biophys. Res. Commun. 71. 952-958.
  • 15. Mann, T. (1964) The Biochemistry oi Semen and the Male Reproductive Tract. Methuen and Co., London, pp. 265-292.
  • 16. Mann, T., Jones, R., Sherins, R. J. (1980) Oxygen damage, lipid peroxidation and motility of spermatozoa, to Testicular Development, Structure and Function. Eds. A. Steinberg, E. Steinberg, pp. 497-501, Raven Press, New York.
  • 17. Paglia, D. E., Valentine, W. N. (1967) Studies on the quantitative and qualitative characterization of erythrocytes glutathione peroxidase. J. Lab. Clin. Med. 70. 158-169.
  • 18. Pederson, N. D., Whanger, P. D., Weswig, P. H. (1975) Effect of dietary selenium depletion and repletion on glutathione peroxidase levels and rat tissues. Nutr. Rep. Int. 11. 429-435.
  • 19. Reitman, S., Frankel, S. (1957) A colorimetric method for the determination of serum glutamic-oxalactic and glutamic pyruvic transaminase. Amer. J. Clin. Path. (Baltimore) 28. 56.
  • 20. Senger, P. L. (1980) Influence of selenium supplementation bovine sperm and seminal quality. Adv. Anim. Breeder 28. 4.
  • 21. Sies, H., Wendel, A., Burk, F. R. (1982) Se and non-Se glutathione peroxidases: enzymology and cell physiology, to Oxidases and Related Redox System. Eds. T. E. King et al., pp. 169-187, Oxford, New York.
  • 22. Smith, D. C., Senger, P. L., MoCutchan, J. F., Landa, С. A. (1979) Selenium and glutathione peroxidase, distribution in bovine semen and selenium-75 retention by the tissues of the reproductive tract in the bull. Biol. Reprod. 20. 377-383.
  • 23. Snedecor, G. W., Cochran, W. G. (1967) Statistical Methods, pp. 172-198. Iowa State University Press.
  • 24. Watson, P. F., Martin, J. C. (1972) A comparison of changes in the acrosomes of deep-frozen ram and bull spermatozoa. J. Reprod. Fert. 28. 99-101.

Typ dokumentu

Bibliografia

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