Calcium involvement in nucleus incertus activation following administration of orexin A

• Autorzy: Siwiec M. , Gundlach A. L. , Lewandowski M. H. , Blasiuk A.
• Języki publikacji: EN

Abstrakty

EN

The nucleus incertus (NI) is a group of GABAergic neurons located in the midline tegmentum. It is the main source of the neuropeptide relaxin-3, which has been shown to be involved in appetite control, modulation of arousal and stress responses, as well as hippocampal theta rhythm. This is similar to the profile of orexins – neuropeptides expressed in the lateral and perifornical hypothalamus. Orexin neurons innervate numerous brain areas, including the brainstem, and activate g-protein-coupled receptors OXR1 and OXR2. This can lead to cell membrane depolarization through a number of possible mechanisms, including increases in intracellular calcium levels. We performed whole-cell patch clamp recordings from NI neurons in rat brain slices. To examine mechanisms of orexin receptor activation, recordings were made using TTX and calcium channel blockers: nickel chloride and nifedipine. We found the depolarizing effect of orexin A on NI neurons was reduced in the presence of calcium channel blockers. These findings help better understand the nature of interactions between the two peptide systems. Combined with further research, they could shed light on the possible involvement of the NI relaxin-3 system in the interplay between arousal, feeding and spatial memory.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.39

Twórcy

autor

Siwiec M.

• Department of Neurophysiology and Chronobiology, Jagiellonian University, Krakow, Poland

autor

Gundlach A. L.

• The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Victoria, Australia

autor

Lewandowski M. H.

• Department of Neurophysiology and Chronobiology, Jagiellonian University, Krakow, Poland

autor

Blasiuk A.

• Department of Neurophysiology and Chronobiology, Jagiellonian University, Krakow, Poland