Plant regeneration via protocorm-like body formation and shoot multiplication from seed-derived callus of a maudiae type slipper orchid

• Autorzy: Hong P.-I. , Chen J.-T. , Chang W.-C.
• Języki publikacji: EN

Abstrakty

EN

Tiny seeds from 5-month-old green capsules of a maudiae type slipper orchid, Paphiopedilum Alma Gavaert, were induced to form totipotent callus on ½ strength MS medium supplemented with 22.60 μM 2,4-D and 4.54 μM TDZ in darkness. The callus was proliferated more and maintained without any morphogenesis on the same medium with a 2-month interval of subculture for more than 2 years. When transferred to 1/2 MS medium supplemented with 26.85 μM NAA, an average of 4.7 protocorm-like bodies (PLBs)/shoot buds formed from each explant after 120 days of culture. After another 72 and 240 days of culture on the same medium, 25 shoot buds and eventually 75 plantlets were obtained through shoot multiplication from the original culture. Kinetin at 4.65 μM was suitable for shoot multiplication and could induce an average of 3.0 shoots from a single young shoot after 60 days of culture. The regenerated plantlets grew normally when transplanted to containers with sphagnum moss in a shaded greenhouse.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 5
• Opis fizyczny: p.755-759,fig.,ref.

Twórcy

autor

Hong P.-I.

• Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China

autor

Chen J.-T.

• Institute of Biotechnology, National University of Kaohsiung, 811 Kaohsiung, Taiwan, Republic of China

autor

Chang W.-C.

• Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China

Bibliografia

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