In vitro propagation, genetic and secondary metabolite analysis of Aconitum violaceum Jacq.: a threatened medicinal herb

• Autorzy: Rawat J.M. , Rawat B. , Agnihotri R. K. , Chandra A. , Nautiyal S.
• Języki publikacji: EN

Abstrakty

EN

Aconitum violaceum Jacq. is an important medicinal species used for various health ailments including renal pain, rheumatism and high fever. In the present report, a reproducible in vitro regeneration system for Aconitum violaceum Jacq. has developed from the nodal segment of the plant. Induction of shoot buds was achieved on basal Murashige and Skoog (MS) medium. The shoots were elongated on MS medium supplemented with 0.5 μM 6-benzylaminopurine (BAP) and 0.1 μM α-napthaleneacetic acid (NAA) and subsequently transferred to rooting medium. In vitro grown microshoots of A. violaceum were encapsulated in the alginate beads. The success rate of their re-growth was found to be approximately 85.43 %. Of the encapsulated microshoots, 39.86 % exhibited formation of multiple shoots following re-growth on plant growth regulator free MS medium. Healthy root formation was observed in all microshoots following 2 weeks of transfer on half-strength MS medium containing 0.1 lM indole-3- acetic acid (IAA) and 1.0 μM α-naphthalene acetic acid (NAA). These plants were subsequently transferred to pots containing a mixture of soil, sand and compost (1:1:1 v/v), and same were then shifted in the greenhouse with 87 % survival rate. The molecular analysis was carried out using 35 random amplified polymorphic DNAs (RAPD) primers and 25 inter simple sequence repeats (ISSR) primers. Cluster analysis of the RAPD and ISSR profile revealed an average similarity coefficient of 0.966 and 0.974, respectively, confirming genetic stability of tissue culture-raised (TR) plants and synthetic seed-derived plants (SR). The phytochemical analysis of tissue culture-raised and synthetic seeds-derived plants showed higher aconitine content than control plant. The propagation protocol developed in this study provides a basis for germplasm conservation and harnessing the medicinally active compounds of A. violaceum.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 08
• Opis fizyczny: p.2589-2599,fig.,ref.

Twórcy

autor

Rawat J.M.

• Botany Division, Forest Research Institute, Dehradun, Uttarakhand, India
• Society for the Conservation of Nature, Parnkuti Anantpur, University Road, Rewa 486 002, Madhya Pradesh, India

autor

Rawat B.

• Society for the Conservation of Nature, Parnkuti Anantpur, University Road, Rewa 486 002, Madhya Pradesh, India
• GB Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, Uttarakhand, India

autor

Agnihotri R. K.

• Department of Botany, Dr. BR Ambedkar University, Agra, Uttar Pradesh, India

autor

Chandra A.

• Botany Division, Forest Research Institute, Dehradun, Uttarakhand, India

autor

Nautiyal S.

• Botany Division, Forest Research Institute, Dehradun, Uttarakhand, India

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