The mutual influence of proteins from Varroa destructor extracts and from honeybee haemolymph on their proteolytic activity – in vitro study

• Autorzy: Fraczek R.J. , Zoltowska K. , Lipinski Z. , Dmitryjuk M.
• Języki publikacji: EN

Abstrakty

EN

The influence of extracts from Varroa destructor, a parasitic mite of the honeybee Apis mellifera, on the proteinase activity of worker bee haemolymph was analysed in vitro, along with the influence of bee haemolymph on the proteolytic activity of V. destructor extract. The study was conducted in three different environments: pH 7.5 (high activity of bee enzymes and very low activity of parasite enzymes), pH 5 (moderate activity of enzymes from both sources) and pH 3.5 (limited activity of bee proteinases and high activity of mite proteinases). Based on electrophoretic studies, the inhibition of the activity of bee haemolymph proteinases by V. destructor extracts was observed at each pH. The study at pH 7.5 with commercial inhibitors of the 4 main classes of proteinases (pepstatin A, ethylenediaminetetraacetic acid (EDTA), E-64 (trans-epoxysuccinyl-L-leucylamido-(4-guanidino)-butane), soybean trypsin inhibitor and Kunitz inhibitor) suggested that parasite extracts mainly inhibited serine proteinases and, to a lower degree, cysteine and aspartyl proteinases. At pH 3.5 and pH 5, a decrease of approximately 40% in parasite proteinase activity was also observed in the presence of bee haemolymph. The result points to the presence of aspartyl proteinase inhibitors in bee haemolymph, which may be an important defence element for bees during food intake by a mite. It was demonstrated that trypsin and trypsin inhibitors are active in the excretion/secretion products of V. destructor, the proteinases of which may assist the parasite in food suckling by preventing haemolymph coagulation, among other things.

Słowa kluczowe

EN

Apis mellifera; honey bee; inhibitor; proteinase; Varroa destructor; hemolymph; proteolytic activity; serine proteinase

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 3
• Opis fizyczny: p.317-323,fig.,ref.

Twórcy

autor

Fraczek R.J.

• Biochemistry Department, Faculty of Biology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-719 Olsztyn, Poland

autor

Zoltowska K.

• Biochemistry Department, Faculty of Biology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-719 Olsztyn, Poland

autor

Lipinski Z.

• Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Division of Reproductive Biology, Tuwima Str.10, 10-748 Olsztyn, Poland

autor

Dmitryjuk M.

• Biochemistry Department, Faculty of Biology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-719 Olsztyn, Poland

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