Evaluation of Neisseria meningitidis carriage with the analysis of serogroups, genogroups and clonal complexes among Polish soldiers

• Autorzy: Korzeniewski K. , Konior M.
• Języki publikacji: EN

Abstrakty

EN

Neisseria meningitidis is an etiological factor of invasive meningococcal disease (IMD). This Gram-negative diplococcus is transmitted from person to person via droplets or through a direct physical contact with secretions of infected patients or asymptomatic carriers. The latter account for 5–10% of the general population. The aim of the study was to estimate the actual N. meningitidis carriage rate in the military environment with identification of serogroups, genogroups, sequence types and clonal complexes of the isolates detected among Polish soldiers. The study was conducted during winter seasons of 2015 and 2016 and involved 883 professional soldiers from the Armoured Brigade in Świętoszów, Poland. The material for testing were nasopharyngeal swabs obtained from study participants. The samples were tested using standard microbiological methods (culture, incubation, microscopy, biochemical and automated identification). N.meningitidis isolates were subjected to slide agglutination test (identification of serogroups), the bacterial DNA was extracted and allowed to determine genogroups, clonal complexes and sequence types. 76 soldiers were found to be carriers of N. meningitidis, they accounted for 8.6% of the study group. The meningococcal isolates mostly belonged to serogroup B. Sequence types ST-11439, ST-136, ST-1136 and the clonal complex 41/44CC were found to be predominant. Clonal complexes responsible for IMD were detected in 15.8% of carriers and 1.4% of the whole study participants. Carriage rates of N. meningitidis among Polish soldiers were found to be similar to those reported in the general population.

Słowa kluczowe

EN

Neisseria meningitidis; etiological factor; invasive meningococcal disease; serogroup; genogroup; clonal complex; Polish soldier; soldier

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2018
• Tom: 67
• Numer: 4
• Opis fizyczny: p.493-500,fig.,ref.

Twórcy

autor

Korzeniewski K.

• Department of Epidemiology and Tropical Medicine, Military Institute of Medicine, Warsaw, Poland

autor

Konior M.

• Department of Epidemiology and Tropical Medicine, Military Institute of Medicine, Warsaw, Poland

Bibliografia

• Andersen J, Barthelsen L, Bech Jensen B, Lind I. 1998. Dynamics of the meningococcal carrier state and characteristics of the carrier strains: a longitudinal study within three cohorts of military recruits. Epidemiol Infect. 121:85–94.
• Ataee RA, Mehrabi-Tavana A, Hosseini SM, Kaviani F. 2016. Carriage rates of Neisseria meningitidis serogroups: determination among freshmen conscripts before vaccination. Iran J Microbiol. 8(3):203–209.
• Blackwell CC, Weir DM, James VS, Todd WTA, Banatvala N, Chaudhuri AKR, Gray HG, Thomson EJ, Fallon RJ. 1990. Secretor status, smoking and carriage of Neisseria meningitidis. Epidemiol Infect. 104(2):203–209.
• Broderick MP, Faix DJ, Hansen CJ, Blair PJ. 2012. Trends in meningococcal disease in the United States military, 1971–2010. Emerg Infect Dis. 18:1430–1437.
• Broderick MP, Phillips C, Faix D. 2015. Meningococcal disease in US Military personnel before and after adoption of conjugate vaccine. Emerg Infect Dis. 21(2):377–379.
• Caugant DA, Høiby EA, Rosenqvist E, Frøholm LO, Selander RK. 1992. Transmission of Neisseria meningitidis among asymptomatic military recruits and antibody analysis. Epidemiol Infect. 109: 241–253.
• Caugant DA, Kristiansen BE, Froholm LO, Bovre K, Selander RK. 1988. Clonal diversity of Neisseria meningitidis from a population of asymptomatic carriers. Infect Immun. 56:2060–2068.
• Caugant DA, Maiden MC. 2009. Meningococcal carriage and disease-population biology and evolution. Vaccine. 27 Suppl 2: B64–B70.
• Caugant DA, Tzanakaki G, Kriz P. 2007. Lessons from meningococcal carriage studies. FEMS Microbiol Rev. 31:52–63.
• Chapalain JC, Guibourdenche M, Perrier-Gros-Claude JD, Bartoli M, Riou JY. 1992. The chemoprophylaxis of cerebrospinal meningitis using rifampin in a military population. Pathol Biol. 40:230–233.
• Claus H, Maiden MC, Wilson DJ, McCarthy ND, Jolley KA, Urwin R, Hessler F, Frosch M, Vogel U. 2005. Genetic analysis of meningococci carried by children and young adults. J Infect Dis. 191:1263–1271.
• Decker MD. 2016. Meningococcal Disease in US Military personnel before and after adoption of conjugate vaccine. Emerg Infect Dis. 22(11):2027–2028.
• Duron S, Martinaud C, Lacassagne D, Koeck JL. 2016. A fatal case of invasive infection caused by w135 Neisseria meningitidis in a vaccinated french soldier. Mil Med. 181(11):e1702–e1705.
• Eslami-Nejad Z, Esmaili M, Saiid-Adeli N, Iranmanesh Z, Honarvar S, Phanii M. 2005. Neisseria meningitidis carrier rate among military recruits in Kerman, South-east of Iran. Arch Iran Med. 8:304–331.
• Fraser PK, Bailey GK, Abbott JD, Gill JB, Walker DJ. 1973. The meningococcal carrier-rate. Lancet 1(7814):1235–1237.
• Grecki M, Bienias M. 2006. Outbreak of invasive meningococcal disease among soldiers in Skwierzyna, Poland, March 2006. Euro Surveill. 11(7):E060706.4
• Guiver M, Borrow R, Marsh J, Gray SJ, Kaczmarski EB, Howells D, Boseley P, Fox AJ. 2000. Evaluation of the Applied Biosystems automated Taqman polymerase chain reaction system for the detection of meningococcal DNA. FEMS Immunol Med Microbiol. 28:173–179.
• Jafri RZ, Ali A, Messonnier NE, Tevi-Benissan C, Durrheim D, Eskola J, Fermon F, Klugman KP, Ramsay M, Sow S, et al. 2013. Global epidemiology of invasive meningococcal disease. Popul Health Metr. 11(1):17.
• Jandova Z, Musilek M, Vackova Z, Kozakova J, Krizova P. 2016. Serogroup and clonal characterization of Czech invasive Neisseria meningitidis strains isolated from 1971 to 2015. PLoS One 11(12): e0167762.
• Kadłubowski M, Waśko I, Klarowicz A, Hryniewicz W. 2007. Invasive meningococcal disease at a military base in Warsaw, January 2007. Euro Surveill. 12(3):E070301.2.
• Konior M, Korzeniewski K. 2016. Analysis of Neisseria meningitidis carriage with identification of sero- and genogroups in environment of professional soldiers. Lek Wojsk. 94(3):239–246 [in Polish].
• Korzeniewski K, Konior M, Kiedrowska M, Wódka E, Zwolińska E, Skoczyńska A. 2017. Prevalence of Neisseria meningitidis carriage with identification of serogroups and genogroups in professional soldiers. Adv Exp Med Biol. 955:29–37.
• Korzeniewski K, Skoczyńska A, Guzek A, Konior M, Chciałowski A, Waśko I, Markowska M, Zwolińska E. 2015. Effectiveness of immunoprophylaxis in suppressing carriage of Neisseria meningitidis in the military environment. Adv Exp Med Biol. 836:19–28.
• Millar BC, Moore PJA, Moore JE. 2017. Meningococcal disease: has the battle been won? J R Army Med Corps. 163(4):235–241.
• Moxon ER, Jansen VA. 2005. Phage variation: understanding the behaviour of an accidental pathogen. Trends Microbiol. 13:563–565.
• NIZP-PZH. 2018. Zachorowania na wybrane choroby zakaźne w Polsce od 1 stycznia do 31 grudnia 2017r. [Internet]. Warszawa (Poland): Zakład Epidemiologii, Narodowy Instytut Zdrowia Publicznego – Państwowy Zakład Higieny, Departament Zapobiegania oraz Zwalczania Zakażeń i Chorób Zakaźnych u Ludzi, Główny Inspektorat Sanitarny; [cited 2018 May 6]. Available from http://www.old.pzh.gov.pl/oldpage/epimeld/2017/INF_17_12B.pdf [in Polish].
• PubMLST. 2018. [access 2018 May 6]. Available from https://pubmlst.org
• Rosenstein NE, Perkins BA, Stephens DS, Popovic T, Hughes JM. 2001. Meningococcal disease. N Engl J Med. 344(18):1378–1388.
• Skoczyńska A, Hryniewicz W. 2012. Meningococcal infections. Pol Merk Lek. 191:283–285. [in Polish].
• Skoczyńska A, Waśko I, Kuch A, Kadłubowski M, Gołębiewska A, Foryś M, Markowska M, Ronkiewicz P, Wasiak K, Kozińska A, et al.; participants of of a laboratory-based surveillance of community acquired invasive bacterial infections (BINet). 2013. A decade of invasive meningococcal disease surveillance in Poland. PLoS One 8(8):e71943.
• Soriano-Gabarro M, Wolter J, Hogea C, Vyse A. 2011. Carriage of Neisseria meningitis in Europe: a review of studies undertaken in the region. Expert Rev Anti Infect Ther. 9(9):761–774.
• Stephens DS. 1999. Uncloaking the meningococcus: Dynamics of carriage and disease Lancet 353(9157):941–942.
• Taha MK. 2000. Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis. J Clin Microbiol. 38:855–857.
• Trotter CL, Chandra M, Cano R, Larrauri A, Ramsay ME, Brehony C, Jolley KA, Maiden MC, Heuberger S, Frosch M. 2007. A surveillance network for meningococcal disease in Europe. FEMS Microbiol Rev. 31(1):27–36.
• Tryfinopoulou K, Kesanopoulos K, Xirogianni A, Marmaras N, Papandreou A, Papaevangelou V, Tsolia M, Jasir A, Tzanakaki G. 2016. Meningococcal carriage in military recruits and university students during the Pre MenB vaccination era in Greece (2014–2015). PLoS One 11(12):e0167404.
• Tyski S, Grzybowska W, Dulny G, Berthelsen L, Lind I. 2001. Phenotypical and genotypical characterization of Neisseria meningitidis carrier strains isolated from Polish recruits in 1998. Eur J Clin Microbiol Infect Dis. 20:350–353.
• Tyski S, Grzybowska W, Dulny G. 2000. Tests for Neisseria meningitidis in adolescents and adults (recruits). Med Dosw Mikrobiol. 52:247–255. [in Polish].
• Tzeng YL, Stephens DS. 2000. Epidemiology and pathogenesis of Neisseria meningitidis. Microbes Infect. 2:687–700.
• Waśko I, Hryniewicz W, Skoczyńska A. 2015. Significance of meningococcal hyperinvasive clonal complexes and their influence on vaccines development. Pol J Microbiol. 64(4):313–321.

Identyfikatory

DOI

10.21307/pjm-2018-058