Performance of the diagnostic test for Demodex spp. in the context of minimizing the risk of false negative results

• Autorzy: Przydatek-Tyrajska R. , Bartosik K. , Sedzikowska A.
• Języki publikacji: EN

Abstrakty

EN

Demodex species are permanent parasites of pilosebaceous units infesting humans worldwide. Of the two species associated with the human host, Demodex folliculorum is much more commonly detected and occurs more abundantly in hair follicles and Zeiss glands. Demodex brevis, on the other hand, is mainly associated with the sebaceous and meibomian glands, all over the body. The infestation is usually chronic, and the troublesome ocular and skin symptoms often make it difficult for patients to function in both a social and professional context. Despite this, diagnostics for Demodex spp. are still not routinely performed during ophthalmological and dermatological examinations. We aimed to compare preparations of eyebrow hair, eyelashes, and skin scrapings, and to investigate the correlation with false negative results in the laboratory diagnosis of demodicosis. Direct microscopic examination was applied on 100 patients (81 women and 19 men). The most common ocular and skin symptoms reported by the study participants were eyelid itching and erythema, respectively. Significant differences were found between infested and uninfested patients with regard to the following symptoms: eyelid itching which was more common in infested patients (35.8%) (p = 0.0343) and chalazion occurring only in infested patients (13.2%) (p = 0.0285). The collection of diagnostic material from more than one site e.g. nasolabial folds, eyebrow hairs or eyelashes has a significant effect on the test result. Taking a single type of a sample from a single face zone can lead to false negative results.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2024
• Tom: 70
• Numer: 4
• Opis fizyczny: p.173-180,fig.,ref.

Twórcy

autor

Przydatek-Tyrajska R.

• RenoMed, Non-Public Health Care Centre, Podkowy 87, 04-937 Warsaw, Poland

autor

Bartosik K.

• Department of Biology and Parasitology, Faculty of Health Sciences, Medical University of Lublin, Radziwiłłowska 11, 20-080 Lublin, Poland

autor

Sedzikowska A.

• Department of General Biology and Parasitology, Medical University of Warsaw, Chałubińskiego 5, 02-004 Warsaw, Poland

Identyfikatory

DOI

10.17420/ap7004.537