Genetic variability in pitch pine (Pinus rigida Mill.) growing in the Niepolomice Forest as determined by ISSR markers

• Autorzy: Masternak K. , Banach J. , Glebocka K. , Wajdzik M.
• Języki publikacji: EN

Abstrakty

EN

The study aimed to determine the genetic variability in pitch pine (Pinus rigida Mill.) growing in the Niepołomice Forest (southern Poland). In the late nineteenth and early twentieth century, Adolf Cieślar of the Department of Forestry Research in Mariabrunn near Vienna, Austria established the experimental crops of pitch pine. During the study, 227 trees that grew in seven subunits were considered; an analysis of genetic polymorphism using the intersimple sequence repeats (ISSR) technique revealed that pitch pine is genetically variable. The average number of alleles at a given locus for all the pine trees was 1.649, while the effective number of alleles at the loci was 1.435. The value of expected heterozygosity was 0.254, while the percentage of polymorphic loci was 75.30%. The average genetic distance between the examined pines was 0.082. Principal coordinate analysis (PCoA) divided the examined pines into three groups, which was also confirmed by the structure-analysis results of the software STRUCTURE. The resulting division was mainly generated by the SR70 primer, which was indicated to be the primer that differentiated the examined populations of pitch pine. Affiliation of particular trees to selected groups was based on their occurrence in individual crops. This suggests a different origin of the seeds used to establish the research plots of pitch pine in the Niepołomice Forest.

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2018
• Tom: 87
• Numer: 3
• Opis fizyczny: Article 3593 [12p.],fig.,ref.

Twórcy

autor

Masternak K.

• Institute of Genetics, Plant Breeding and Biotechnology, Faculty of Agrobioengineering, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland

autor

Banach J.

• Department of Genetics and Forest Tree Breeding, Institute of Forest Ecology and Silviculture, Faculty of Forestry, University of Agriculture in Krakow, 29 Listopada 46, 31-425 Krakow, Poland

autor

Glebocka K.

• Institute of Genetics, Plant Breeding and Biotechnology, Faculty of Agrobioengineering, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland

autor

Wajdzik M.

• Department of Genetics and Forest Tree Breeding, Institute of Forest Ecology and Silviculture, Faculty of Forestry, University of Agriculture in Krakow, 29 Listopada 46, 31-425 Krakow, Poland

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Identyfikatory

DOI

10.5586/asbp.3593