Identification and quantification of phenolic compounds in Hypericum perforatum L. transgenic shoots

• Autorzy: Tusevski O. , Petreska Stanoeva J. , Stefova M. , Pavokovic D. , Gadzovska Simic S.
• Języki publikacji: EN

Abstrakty

EN

Regeneration of transgenic shoots was achieved from Hypericum perforatum L. hairy roots on hormone-free MS/B₅ medium for a period of 4 weeks under a photoperiod of 16-h light. A control experiment was set up with root segments obtained from in vitro grown seedlings. Investigations have been made to study the production of phenolic compounds in non transgenic and transgenic shoot cultures. Six groups of phenolic compounds such as phenolic acids, flavonols, flavan-3-ols, naphtodianthrones, phloroglucinols, and xanthones were recorded in the transgenic shoots. Chlorogenic acid was found as the most representative phenolic acid in shoot extracts. With regard to the class of quercetin derivatives in transformed shoots, quercetin 6-C-glucoside usually dominated among the glycosides followed by quercitrin and hyperoside. The analysis of flavan-3-ols in transgenic shoots resulted in the identification of epicatechin and proanthocyanidin dimers. One of the main achievements in this study was considerably enhanced hypericin and pseudohypericin production in transgenic shoots. The concentration of identified naphtodianthrones was about 12-fold higher in transformed shoots compared to control. Chromatographic analysis of phloroglucinols in transgenic shoots resulted in the identification of hyperforin, while its homolog adhyperforin was detected in traces. A twofold higher content of hyperforin was observed in transgenic shoots compared to control. Although mangiferin was found as the main representative xanthone in shoot extracts, several other xanthones identified as γ-mangostin isomers, trihydroxy-1-methoxy-C-prenyl xanthone, garcinone E, and banaxathone E were de novo synthesized in transformed shoots. Therefore, H. perforatum transgenic shoots could be considered as a source for rapid and increased production of naphtodianthrones and other specific phenolic compounds.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 10
• Opis fizyczny: p.2555-2569,fig.,ref.

Twórcy

autor

Tusevski O.

• Department of Plant Physiology, Faculty of Natural Sciences and Mathematics, Institute of Biology, “Ss. Cyril and Methodius” University, Skopje, 1000, Macedonia

autor

Petreska Stanoeva J.

• Department of Analytical Chemistry, Faculty of Natural Sciences and Mathematics, Institute of Chemistry, “Ss. Cyril and Methodius” University, Skopje, 1000, Macedonia

autor

Stefova M.

• Department of Analytical Chemistry, Faculty of Natural Sciences and Mathematics, Institute of Chemistry, “Ss. Cyril and Methodius” University, Skopje, 1000, Macedonia

autor

Pavokovic D.

• Department of Molecular Biology, Faculty of Sciences, University of Zagreb, Horvatovac 102a, HR 10000, Zagreb, Croatia

autor

Gadzovska Simic S.

• Department of Plant Physiology, Faculty of Natural Sciences and Mathematics, Institute of Biology, “Ss. Cyril and Methodius” University, Skopje, 1000, Macedonia

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Identyfikatory

DOI

10.1007/s11738-014-1627-4