Cryopreservation of bull semen in OptiXcell and conventional extenders: Comparison of semen quality and fertility

• Autorzy: Ansari M.S. , Rakha B.A. , Akhter S.
• Języki publikacji: EN

Abstrakty

EN

Commercially available OptiXcell® extender was compared with conventional extenders for freezability and in vivo fertility of bull semen. Semen was collected from three Friesian bulls for five weeks (replicate) and qualifying ejaculates (motility >60%, concentration >0.5 billion/mL, volume >lmL) were diluted (37°C; 50 x 10⁶ spermatozoa/ml) with OptiXcell®, tris-citric egg yolk and egg yolk-citrate extenders. Diluted semen was cooled to 4°C in 2 hours, equilibrated for 4 hours and filled in 0.5 ml straws. The straws were kept over liquid nitrogen vapours for 10 minutes and plunged into liquid nitrogen. Percentages of post thaw sperm motility and plasma membrane integrity were recorded higher (P<0.05) in OptiXcell® compared to tris-citric egg yolk followed by egg yolk-citrate extender. Sperm viability (%) were recorded higher (P<0.05) in OptiXcell® compared to tris-citric egg yolk and egg yolk citrate extender. Percentages of normal apical ridge and DNA integrity were higher (P<0.05) in OptiXcell® and tris-citric egg yolk extender compared to egg yolk-citrate extender. Higher (P<0.05) fertility rate was recorded with semen frozen in OptiXcell® compared to triscitric egg yolk and egg yolk-citrate extender. In conclusion, OptiXcell® is superior to conventional extenders for spermatozoa) quality of frozen-thawed bull semen and produced higher fertility rates under field conditions.

Słowa kluczowe

EN

cryopreservation; bull; semen; OptiXcell extender; conventional extender; semen quality; fertility; artificial insemination

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2017
• Tom: 35
• Numer: 3
• Opis fizyczny: p.317-328,fig.,ref.

Twórcy

autor

Ansari M.S.

• Department of Zoology, University of Sargodha, Lyallpur Campus, Faisalabad-37000, Pakistan

autor

Rakha B.A.

• Department of Wildlife Management, Pir Mehr Ali Shah arid Agriculture University, Rawalpindi-46300, Pakistan

autor

Akhter S.

• Department of Zoology, Pir Mehr Ali Shah arid Agriculture University, Rawalpindi-46300, Pakistan

Bibliografia

• 1. AKHTER S., ANSARI M. S., ANDRABI S. M. H., RAKHA B. A., ULLAH N., KHALID M., 2012 - Soya-lecithin in extender improves the freezability and fertility of buffalo (Bubalus bubalis) bull spermatozoa. Reproduction in Domestic Animals 47, 815 - 819.
• 2. AKHTER S., ANSARI M. S., RAKHA B. A., ANDRABI S. M. H., KHALID M., Ullah., 2011 – in vitro evaluation of liquid-stored buffalo semen at 5°C diluted in soya lecithin based extender (Bioxcell®), tris-citric egg yolk, skim milk and egg yolk-citrate extenders. Reproduction in Domestic Animals 46, 45 - 49.
• 3. AKHTER S., ANSARI M. S., RAKHA B. A., ANDRABI S. M. H., IQBAL S., ULLAH N., 2010 - Cryopreservation of buffalo (Bubalus bubalis) semen in Bioxcell® extender. Theriogenology 74, 951 - 955.
• 4. AKHTER S., ANSARI M. S., RAKHA B. A., ANDRABI S. M. H., QADEER S., IQBAL R., ULLAH N., 2013 - Efficiency of ciprofloxacin for bacterial control, post-thaw quality, and in vivo fertility of buffalo spermatozoa. Theriogenology 80, 378 - 383.
• 5. AKHTER S., RAKHA B. A., ANSARI M. S., HUSNA A. U., IQBAL S., KHALID M., 2016 Evaluation of quail and turkey egg yolk for cryopreservation of Nili-Ravi buffalo bull semen. Theriogenology Doi: 10.1016/j.theriogenology.2016.09.002.
• 6. AMIRAT L., ANTON M., TAINTURIER D., CHATAGNON G., BATTUT I., COURTENS J. L., 2005 Modifications of bull spermatozoa induced by three extenders: Biociphos, low density lipoprotein and Triladyl, before, during and after freezing and thawing. Reproduction 129, 535 - 543.
• 7. AMIRAT L., TAINTURIER D., JEANNEAU L., THORIN C., GERARD O., COURTENS J. L., ANTON M., 2004 - Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl®, a commercial egg yolk extender. Theriogenology 61, 895 - 907.
• 8. AMIRAT-BRIAND L., BENCHARIF D., VERA-MUNOZ O., PINEAU S., THORIN C., DESTRUMELLE S., DESHERCES S., ANTON M., JOUAN M., SHMITT E., TAINTURIER D., 2010 - In vivo fertility of bull semen following cryopreservation with an LDL (low density lipoprotein) extender: Preliminary results of artificial inseminations. Animal Reproduction Science 122, 282 - 287.
• 9. ANSARI M. S., RAKHA B. A., AKHTER S., ASHIQ M., 2016 - OPTIXcell improves the post-thaw quality and fertility of buffalo bull sperm. Theriogenology 85, 528 - 532.
• 10. ANSARI M. S., RAKHA B. A., ANDRABI S. M. H., ULLAH N., IQBAL R., HOLT W. V., AKHTER S., 2012 - Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa. Reproductive Biology 12, 271 - 276.
• 11. ANSARI M. S., RAKHA B. A., ANDRABI S. M. H., AKHTER S., 2010 - Usefulness of powdered and fresh egg yolk for cryopreservation of Zebu bull spermatozoa. Reproductive Biology 10, 235 - 240.
• 12. ANZAR M., KAKUDA N., HE L., PAULS K. P., BUHR M. M., 2002 - Optimizing and quantifying fusion of liposomes to mammalian sperm using resonance energy transfer and flow cytometric methods. Cytometry 49, 22 - 27.
• 13. BELALA R., BRIAND-AMIRAT L., VINCIGUERRA L., TAINTURIER D., KAIDI R., THORIN C., MICHAUD S., ANTON M., BENCHARIF D., 2016a - Effect of equilibration time on the motility and functional integrity of canine spermatozoa frozen in three different extenders. Research in Veterinary Science 106, 66 - 73.
• 14. BELALA R., DELAY J., AMIRAT L., ROPERS M. H., LE GUILLOU J., ANTON M., SCHMITT E., THORIN C., MICHAUD S., KAIDIR., TAINTURIER D., 2016b - The benefits of liposomes for chilling canine sperm for 4 days at 4° C. Animal Reproduction Science 168, 100 - 109.
• 15. BERGERON A., MANJUNATH P., 2006 - New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Molecular Reproduction and Development 73, 1338 - 1344.
• 16. BOUSSEAU S., BRILLARD J. P., MARQUANT-LE GUIENNE B., GUERIN B., CAMUS A., LECHAT M., 1998 - Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluents. Theriogenology 50, 699 - 706.
• 17. BRITO L. F., BARTH A. D., BILODEAU-GOESEELS S., PANÍCH P. L., KASTELIC J. P., 2003 - Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate. Theriogenology 60, 1539 - 1551.
• 18. DAVIS I. S., BRATTON R. W., FOOTE R. H., 1963 - Livability of Bovine Spermatozoa at 5,- 25, and - 85C in tris-buffered and citrate-buffered yolk-glycerol extenders. Journal of Dairy Science 46, 333 - 336.
• 19. DUMAN J. G., 1977 - The role of macromolecular antifreeze in the darkling beetle, Meracantha contracta. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology 115, 279 - 286.
• 20. FOULKES J. A., 1977 - The separation of lipoproteins from egg yolk and their effect on t h e motility and integrity of bovine spermatozoa. Journal of Reproduction and Fertility 49, 277 - 284.
• 21. GRAHAM E. F., CRABO B. G., BROWN K. I., 1972 - Effect of Some Zwitter Ion Buffers on the freezing and storage of spermatozoa. Journal of Dairy Science 55, 372 - 378.
• 22. GRAHAM J. K., FOOTE R. H., 1987 - Effect of several lipids, fatty acyl chain length, and degree of unsaturation on the motility of bull spermatozoa after cold shock and freezing. Cryobiology 24, 42 - 52.
• 23. GRIFFITH M., YAISH M. W., 2004 - Antifreeze proteins in overwintering plants: a tale of two activities. Trends in plant science 9, 399 - 405.
• 24. HU J. H., JIANG Z. L., LV R. K., LI Q. W., ZHANG S. S., ZAN L. S., LI Y. K., LI X., 2011 - The advantages of low-density lipoproteins in the cryopreservation of bull semen. Cryobiology 62, 83 - 87.
• 25. HU J. H., LI Q. W., ZAN L. S., JIANG Z. L., AN J. H., WANG L. Q., JIA Y. H., 2010 - The cryoprotective effect of low-density lipoproteins in extenders on bull spermatozoa following freezing-thawing. Animal Reproduction Science 117, 11 - 17.
• 26. JEYENDRAN R. S., VAN DER VEN H. H., PEREZ-PELAEZ M., CRABO B. G., ZANEVELD L. J. D., 1984 - Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70, 219 - 228.
• 27. KOVÁCS A., FOOTE R. H., 1992 - Viability and acrosome staining of bull, boar and rabbit spermatozoa. Biotechnic and Histochemistry 67, 119 - 24.
• 28. KUMAR P., SAINI M., KUMAR D., BALHARA A. K., YADAV S. P., SINGH P., YADAV P. S., 2015 - Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Animal Reproduction Science 159, 38 - 45.
• 29. MIGUEL-JIMÉNEZ S., MOGAS T., PEŃA A. I., TAMARGO C., HIDALGO C. O., MUIŃO R., RODRÍGUEZ-GIL J. E., MORATÓ R., 2016 - Post-thaw changes in sperm membrane and ROS following cryopreservation of dairy bull semen using four different commercial extenders. Animal Reproduction 13, 573 - 573.
• 30. MANJUNATH P., 2012 - New insights into the understanding of the mechanism of sperm protection by extender components. Animal Reproduction 9, 809 - 815.
• 31. MANJUNATH P., NAUC V., BERGERON A., MÉNARD M., 2002 - Major proteins ofbovine seminal plasma bind to the low-density lipoprotein fraction of hen’s egg yolk. Biology of Reproduction 67, 1250 - 1258.
• 32. MOUSSA M., MARTINET V., TRIMECHE A., TAINTURIER D., ANTON M., 2002 - Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology 57, 1695 - 1706.
• 33. MULLER-SCHLOSSER F., AIRES V., HINSCH E., HINSCH K. D., 2001 -Evaluation of the quality of a new generation of egg yolk free semen diluters for cryopreservation ofbovine semen. Jahrestaung uber Physiologie und Pathologie der Fortpflanzung, p. 54.
• 34. NICHI M., BOLS P. E. J., ZÜGE R. M., BARNABE V. H., GOOVAERTS I. G. F., BARNABE R. C., CORTADA C. N. M., 2006 - Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenology 66, 822 - 828.
• 35. PACE M. M., GRAHAM E. F., 1974 - Components in egg yolk which protect bovine spermatozoa during freezing. Journal of Animal Science 39, 1144 - 1149.
• 36. PILLET E., DUCHAMP G., BATELLIER F., BEAUMAL V., ANTON M., DESHERCES S., SCHMITT E., MAGISTRINI M., 2011 - Egg yolk plasma can replace egg yolk in stallion freezing extenders. Theriogenology 75, 105 - 114.
• 37. PILLET E., LABBE C., BATELLIER E., DUCHAMP G., BEAUMAL V., ANTON M., DESHERCES S., SCHMITT E., MAGISTRINI M., 2012 - Liposomes as an alternative to egg yolk in stallion freezing extender. Theriogenology 77, 268 - 279.
• 38. REKHA A., ZOHARA B. R, BARI F. Y., ALAM M. G. S., 2016 - Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep. Animal Reproduction, 13, 735 - 742.
• 39. ROBINSON C. H., 2001 - Cold adaptation in Arctic and Antarctic fungi. New Phytologist, 151, 341 - 353.
• 40. RÖPKE T., OLDENHOF H., LEIDING C., SIEME H., BOLLWEIN H., WOLKERS W. F., 2011 - Liposomes for cryopreservation of bovine sperm. Theriogenology 76, 1465 - 1472.
• 41. SMITH R. L., BERNDTSON W. E., UNAL M. B., PICKETT B. W., 1979 - Influence of percent egg yolk during cooling and freezing on survival ofbovine spermatozoa. Journal of Dairy Science 62, 1297 - 1303.
• 42. STEINBACH J., FOOTE R. H., 1964 - Post-thaw survival ofbovine spermatozoa frozen by different methods in buffered-yolk and skim milk extenders. Journal of Dairy Science 47, 909 - 915.
• 43. STEWART J. L., SHIPLEY C. F., KATICH A. S., PO E., ELLERBROCK R. E., LIMA F.S., CANISSO I. F., 2016 - Cryopreservation of white-tailed deer (Odocoileus virginianus) semen using soybean-, liposome-, and egg yolk-based extenders. Animal Reproduction Science Dot 10.1016/j.anireprosci.2016.05.006.
• 44. TARTAGLIONE C. M., RITTA M. N., 2004 - Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen. Theriogenology 62, 1245 - 1252.
• 45. URRUTIA M. E., DUMÁN J. G., KNIGHT C. A., 1992 - Plant thermal hysteresis proteins. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology 1121, 199 - 206.
• 46. VAN WAGTENDONK-DE LEEUW A. M., HARING R. M., KAAL-LANSBERGEN L. M. T. E. DEN DAAS J. H. G., 2000 - Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soy bean extract. Theriogenology 54, 57 - 67.
• 47. VISHWANATH R., SHANNON P., 2000 - Storage of bovine semen in liquid and frozen state. Animal Reproduction Science 62, 23 - 53.
• 48. WALL R. J., FOOTE R. H., 1999 - Fertility of bull sperm frozen and stored in clarified egg yolk-Tris- glycerol extender. Journal of Dairy Science 82, 817 - 821.
• 49. WATSON P. F., MARTIN I. C. A., 1976 - Artificial insemination of sheep: the effect of semen diluents containing egg yolk on the fertility of ram semen. Theriogenology 6, 559 - 564.
• 50. WORRALL D., ELIAS L., ASHFORD D., SMALLWOOD M., SIDEBOTTOM C., LILLFORD P., TELFORD J., HOLT C., BOWLES D., 1998 - A carrot leucine-rich-repeat protein that inhibits ice recrystallization. Science 282, 115 - 117.