Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

• Autorzy: Briza J. , Pavingerova D. , Vlasak J. , Niedermeierova H.
• Języki publikacji: EN

Abstrakty

EN

 Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

Słowa kluczowe

EN

Norway spruce; Picea abies; genetic transformation; Cry3A gene; Bacillus thuringiensis; embryogenic tissue; somatic embryogenesis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 3
• Opis fizyczny: p.395-400,fig.,ref.

Twórcy

autor

Briza J.

• Institute of Plant Molecular Biology, Biology Centre AS CR, Ceske Budejovice, Czech Republic
• Faculty of Science, South Bohemia University, Ceske Budejovice, Czech Republic

autor

Pavingerova D.

• Institute of Plant Molecular Biology, Biology Centre AS CR, Ceske Budejovice, Czech Republic

autor

Vlasak J.

• Institute of Plant Molecular Biology, Biology Centre AS CR, Ceske Budejovice, Czech Republic

autor

Niedermeierova H.

• Institute of Plant Molecular Biology, Biology Centre AS CR, Ceske Budejovice, Czech Republic

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