Callus induction and organogenesis in vitro of cattleya from protocorm-like bodies (PLBs) under different light conditions

• Autorzy: Cybularz-Urban T. , Hanus-Fajerska E. , Bach A.

Warianty tytułu

PL

Indukcja kalusa i organogeneza in vitro cattleya z ciał potokormopodobnych (PLBs) w różnych warunkach świetlnych

• Języki publikacji: EN

Abstrakty

EN

In this paper we report the research on the effect of the different light in vitroconditions on growth and development of interspecific hybrid Cattleya intermedia × C. aurantiaca (Orchidaceae) maintained as stock in vitro shoot culture. Callus was obtained from shoots explants on initiation medium which was composed of MS mineral salts and vitamin set, supplemented with 30 g l-1 sucrose, 2.0 mg dm-3 adenine sulphate, 9.7 mg dm-3 ascorbic acid, with addition of 1.0 μM TDZ, whereas callus maintenance medium was instead supplemented with 4.95 μM BA, and 1 μM NAA. Proliferating protocorm-like bodies (PLBs) of that precious plant material were exposed to the irradiation with mono-chromatic light characterized by different wavelength. Some interesting lines have been obtained on white, blue, red, far red, and ultraviolet light respectively, which proved to be diversified in the proliferation rates as well as in the morphological and anatomical features. The light treatment also significantly affected regenerative potential of studied culture. Blue light applied during two subsequent culture passages was proved to be the best option in order to regenerate shoots via PLBs. Nevertheless blue, red and far red irradiation of cultures led to distinctive reduction in the content of chlorophyll and carotenoid pigments, compared with culture irradiated with either white or ultraviolet light.

PL

W pracy opisano badania nad wpływem różnych warunków świetlnych in vitro na wzrost i rozwój międzygatunkowego mieszańca Cattleya intermedia × C. auran-tiaca (Orchidaceae), którego zasób stanowiły kultury pędowe. Kalus indukowano z eks-plantatów pędowych na zestalonej pożywce składającej się z zestawu soli mineralnych i witamin według MS, wzbogaconej 30 g l-1 sacharozy, 2,0 mg dm-3 siarczanu adeniny, 9,7 mg dm-3 kwasu askorbinowego i z dodatkiem 1,0 μM TDZ, podczas gdy w pożywce proliferacyjnej zastosowano 4,95 μM BA, i 1,0 μM NAA. Badany materiał roślinny w formie ciał protokormo-podobnych (PLBs) eksponowano na światło monochromatyczne o zróżnicowanej długości fali świetlnej. Na świetle białym, niebieskim, czerwonym, dalekiej czerwieni i ultrafiolecie uzyskano wartościowe linie, które różniły się tempem proliferacji, morfologią i cechami anatomicznymi. Poszczególne traktowania wywarły również znaczący wpływ na potencjał regeneracyjny badanej kultury. Światło niebieskie okazało się najbardziej korzystne w regeneracji pędów za pośrednictwem PLBs. Naświetlanie kul-tur światłem niebieskim, czerwonym lub daleką czerwienią doprowadziło do znaczącej redukcji zawartości chlorofili i karotenoidów w porównaniu z zawartością tych barwników w materiale eksponowanym na światło białe lub ultrafioletowe.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2015
• Tom: 14
• Numer: 6
• Opis fizyczny: p.29-38,fig.,ref.

Twórcy

autor

Cybularz-Urban T.

• University of Agriculture in Krakow, Krakow, Poland

autor

Hanus-Fajerska E.

• Department of Botany, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Krakow, Poland

autor

Bach A.

• University of Agriculture in Krakow, Krakow, Poland

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