The effect of polyamines on the efficiency of multiplication and rooting of Withania somnifera (L.) Dunal and content of some withanolides in obtained plants

• Autorzy: Sivanandhan G. , Mariashibu T. S. , Arun M. , Rajesh M. , Kasthurirengan S. , Selvaraj N. , Ganapathi A.
• Języki publikacji: EN

Abstrakty

EN

An efficient mass multiplication protocol was developed for Withania somnifera (L.) Dunal from nodal explants of field-grown plants on Murashige and Skoog medium (MS) supplemented with 6-benzyladenine (BA) [1.5 mg L⁻¹], indole-3-acetic acid (IAA) [0.3 mg L⁻¹] and with the addition of polyamine, spermidine (20 mg L⁻¹) (shoot multiplication medium). A total of 46.4 shoots were obtained from nodal explants and they were elongated in the same medium in a culture duration of 6 weeks. The elongated shoots produced roots in MS medium fortified with putrescine (20 mg L⁻¹) after 4 weeks, and all the rooted plants were successfully hardened and acclimatized with a survival rate of 100%. An average of 276 shoots (46 × 6) was produced when at least six nodal explants obtained from each of the 46 in vitro grown shoots were cultured by microcutting method in the same shoot multiplication medium. On an average, 12,696 plants could be produced from all the shoots (276 × 46) by microcuttings in a period of 7 months. HPLC revealed a significant increase in the quantities of withanolide A, withanolide B, withaferin A and withanone in the leaves, stems, and roots of in vitro regenerated plants compared to the field-grown parent plants. Ploidy analysis using flow cytometry revealed genetic stability of in vitro regenerated plants. This protocol will be useful for scale-up production of withanolides on commercial scale.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 6
• Opis fizyczny: p.2279-2288,fig.,ref.

Twórcy

autor

Sivanandhan G.

• Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Mariashibu T. S.

• Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Arun M.

• Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Rajesh M.

• Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Kasthurirengan S.

• Temasek Life Sciences Laboratory Limited, 1 Research Link National University of Singapore, Singapore 117604, Singapore

autor

Selvaraj N.

• Periyar E.V.R College, Tiruchirappalli 620 023, India

autor

Ganapathi A.

• Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

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